Overexpression of wheat dehydrin DHN-5 enhances tolerance to salt and osmotic stress in Arabidopsis thaliana

Brini, Faïçal; Hanin, Moez; Lumbreras, Victoria; Amara, Imen; Khoudi, Habib; Hassairi, Afif; Pagès, Montserrat; Masmoudi, Khaled

doi:10.1007/s00299-007-0412-x

Cited by 215 publications

(144 citation statements)

References 41 publications

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“…Most of the barley Dhn genes are up-regulated by dehydration and ABA, while others are cold-induced or embryo-specific. Also, the ectopic expression of a wheat dehydrin (DHN-5) in Arabidopsis plants improved their tolerance to high salinity and water deficit (Brini et al, 2007). Our results underline the fact that during drought stress, variations in pattern expression of dehydrin gene (LEA group2) are observed.…”

Section: Dehydrins (Dhns)supporting

confidence: 61%

Analysis of mRNA Levels of Ten Genes Under Water Stress in Triticum turgidum subsp. durum

Melloul¹,

Iraqi²,

Udupa³

et al. 2013

JPS

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Drought is one of the major causes of dramatic yield loss in crop plants. Plants perceive and respond to stress. Upon perception of stress, a signal is communicated to downstream components resulting in change of gene expression and thereby of proteins required for the initial damage-repair and physiological re-programming for better adaptation. In this work, a set of 10 genes from Triticum turgidum subsp. durum, were tested for their expression under drought conditions. These drought responsive genes selected for expressional analyses can be classified into two groups. The first group includes functional proteins already known to be involved in the response to water stress such as late embryogenesis protein. The second group comprises protein factors involved in the regulation of signal transduction and gene expression, such as transcription factors. We have used the real-time quantitative PCR to monitor the expression patterns of these 10 genes in Triticum durum leaves under drought stress. The results showed a high quantitative up-regulation of some genes belonging to dehydrin, transcription factors (DREBS), cell wall polysaccharides and regulation of secretion categories. Moreover, the actin binding protein and an ethylene-responsive element binding factor genes were slightly down-regulated and not significantly affected by the drought stress. The result of this study extended our knowledge of drought induced genes and may provide better understanding of the molecular mechanisms of drought response inTriticum durum. Identifying genes turned on or off in response to water stress will help in enhancing drought tolerance and providing genes that can be tested by many biotechnology-based approaches. The long term objective of this study is to choose the optimum condition for subsequent whole transcriptomic analysis by cDNA-AFLP.

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Section: Dehydrins (Dhns)supporting

confidence: 61%

Analysis of mRNA Levels of Ten Genes Under Water Stress in Triticum turgidum subsp. durum

Melloul¹,

Iraqi²,

Udupa³

et al. 2013

JPS

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“…Kaye et al 61 reported that tobacco plants expressing spinach CAP85 and CAP160 DHNs revealed a lower level of electrolyte leakage after a frost test, which indicates a reduction of freezing injury in the transformants. Brini et al 62 have observed that the expression of the durum wheat (Triticum turgidum ssp. durum) DHN-5 in A. thaliana led to an increase in salt and osmotic stress tolerance.…”

Section: Modulation Of Plant Defense Responses By Dehydrinsmentioning

confidence: 99%

Plant dehydrins and stress tolerance

Hanin

Brini

Ebel

et al. 2011

Plant Signaling & Behavior

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408

195

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“…The identified LEA proteins did not show any differences in the expression pattern of tested genotypes between stressed and control plants (Witzel et al 2009). In another study it was shown that the expression of the durum wheat dehydrin DHN5 in A. thaliana led to an increase in tolerance toward salt and osmotic stress in transgenic plants (Brini et al 2007). Likewise, increased tolerance to drought and salinity was also observed in tobacco expressing Rab16A LEA gene from salt-tolerant rice (RoyChoudhury et al 2007).…”

Section: Late Embryogenesis Abundant Proteinsmentioning

confidence: 99%

Influence of abiotic stresses on plant proteome and metabolome changes

et al. 2013

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Plant responses to abiotic stresses are very complex phenomena with individual characteristics for various species. Abiotic stresses (e.g. drought, salinity, flooding, cold, heat, UV radiation, heavy metals, etc.) strongly affect plant growth and development. It is estimated that they are the cause of more than 50 % of crop yield losses. Abiotic stresses are known to activate a multigene response resulting in the changes in various proteins and primary and secondary metabolite accumulation. Therefore, proteomic and metabolomic approaches are becoming very important and powerful tools used in studying plants' reaction to various stimuli. Precise analysis of proteome and metabolome is essential for understanding the fundamentals of stress physiology and biochemistry. In this review, we focus on recent reports concerned to the influence of abiotic stresses on changes in the level of different protein groups and metabolite classes. Basic information about physicochemical methods applied to qualitative and quantitative analyses of biopolymers and natural products is also briefly presented.

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Overexpression of wheat dehydrin DHN-5 enhances tolerance to salt and osmotic stress in Arabidopsis thaliana

Cited by 215 publications

References 41 publications

Analysis of mRNA Levels of Ten Genes Under Water Stress in Triticum turgidum subsp. durum

Analysis of mRNA Levels of Ten Genes Under Water Stress in Triticum turgidum subsp. durum

Plant dehydrins and stress tolerance

Influence of abiotic stresses on plant proteome and metabolome changes

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