Overexpression of watermelon m6A methyltransferase ClMTB enhances drought tolerance in tobacco by mitigating oxidative stress and photosynthesis inhibition and modulating stress-responsive gene expression

He, Yanjun; Li, Yulin; Yao, Yixiu; Zhang, Huiqing; Wang, Yuhuan; Gao, Jie; Fan, Min

doi:10.1016/j.plaphy.2021.10.007

Cited by 15 publications

(6 citation statements)

References 53 publications

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“…In addition, the expression of m 6 A methyltransferase ClMTB in watermelon was also induced by drought stress. Overexpression of ClMTB in tobacco plants increased their drought tolerance by enhancing ROS scavenging activity [15]. Overexpressing Populus trichocarpa m 6 A methyltransferase also enhanced the tolerance to drought stress by increasing the trichome and root density in poplar [13].…”

Section: Discussionmentioning

confidence: 97%

“…However, ClMTB, an AtMTB homolog protein in watermelon, and its expression was induced under drought stress. Overexpression of ClMTB enhanced drought tolerance by elevating the reactive oxygen species (ROS) scavenging ability and alleviating photosynthesis inhibition in tobacco [15]. Both these MTAs and MTBs belong to the MT-A70-like (MT) proteins [3].…”

Section: Introductionmentioning

confidence: 99%

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Comparative Genomics and Functional Studies of Putative m6A Methyltransferase (METTL) Genes in Cotton

Cao

Huang

Liu

et al. 2022

IJMS

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N6-methyladenosine (m6A) RNA modification plays important regulatory roles in plant development and adapting to the environment, which requires methyltransferases to achieve the methylation process. However, there has been no research regarding m6A RNA methyltransferases in cotton. Here, a systematic analysis of the m6A methyltransferase (METTL) gene family was performed on twelve cotton species, resulting in six METTLs identified in five allotetraploid cottons, respectively, and three to four METTLs in the seven diploid species. Phylogenetic analysis of protein-coding sequences revealed that METTL genes from cottons, Arabidopsis thaliana, and Homo sapiens could be classified into three clades (METTL3, METTL14, and METTL-like clades). Cis-element analysis predicated the possible functions of METTL genes in G. hirsutum. RNA-seq data revealed that GhMETTL14 (GH_A07G0817/GH_D07G0819) and GhMETTL3 (GH_A12G2586/GH_D12G2605) had high expressions in root, stem, leaf, torus, petal, stamen, pistil, and calycle tissues. GhMETTL14 also had the highest expression in 20 and 25 dpa fiber cells, implying a potential role at the cell wall thickening stage. Suppressing GhMETTL3 and GhMETTL14 by VIGS caused growth arrest and even death in G. hirsutum, along with decreased m6A abundance from the leaf tissues of VIGS plants. Overexpression of GhMETTL3 and GhMETTL14 produced distinct differentially expressed genes (DEGs) in A. thaliana, indicating their possible divergent functions after gene duplication. Overall, GhMETTLs play indispensable but divergent roles during the growth of cotton plants, which provides the basis for the systematic investigation of m6A in subsequent studies to improve the agronomic traits in cotton.

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Section: Discussionmentioning

confidence: 97%

Section: Introductionmentioning

confidence: 99%

Comparative Genomics and Functional Studies of Putative m6A Methyltransferase (METTL) Genes in Cotton

Cao

Huang

Liu

et al. 2022

IJMS

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“…Recent studies have demonstrated that the infection of tobacco mosaic virus (TMV) has altered the level of m 6 A within tobacco [ 35 ]. Additionally, overexpression m 6 A methyltransferase ClMTB of watermelon enhanced the drought tolerance of tobacco [ 36 ]. However, genes associated with m 6 A modification in tobacco have yet to be identified.…”

Section: Introductionmentioning

confidence: 99%

Genome-wide identification of the N6-methyladenosine regulatory genes reveals NtFIP37B increases drought resistance of tobacco (Nicotiana tabacum L.)

Su,

Meng,

et al. 2024

BMC Plant Biol

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Background N6-methyladenosine (m6A) is one of the common internal RNA modifications found in eukaryotes. The m6A modification can regulate various biological processes in organisms through the modulation of alternative splicing, alternative polyadenylation, folding, translation, localization, transport, and decay of multiple types of RNA, without altering the nucleotide sequence. The three components involved in m6A modification, namely writer, eraser, and reader, mediate the abundance of RNA m6A modification through complex collaborative actions. Currently, research on m6A regulatory genes in plants is still in its infancy. Results In this study, we identified 52 candidate m6A regulatory genes in common tobacco (Nicotiana tabacum L.). Gene structure, conserved domains, and motif analysis showed structural and functional diversity among different subgroups of tobacco m6A regulatory genes. The amplification of m6A regulatory genes were mainly driven by polyploidization and dispersed duplication, and duplicated genes evolved through purified selection. Based on the potential regulatory network and expression pattern analysis of m6A regulatory genes, a significant number of m6A regulatory genes might play important roles in growth, development, and stress response processes. Furthermore, we have confirmed the critical role of NtFIP37B, an m6A writer gene in tobacco, in enhancing drought resistance. Conclusions This study provides useful information for better understanding the evolution of m6A regulatory genes and the role of m6A modification in tobacco stress response, and lays the foundation for further elucidating the function of m6A regulatory genes in tobacco.

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“…For example, RNA-seq and metabolomic analyses revealed the regulatory network and mechanisms underlying the production of sugars and organic acids in watermelon 10, 18, 26, and 34 days after pollination (Umer et al, 2020). The overexpression of watermelon ClMTB has been found to increase the drought resistance of transgenic tobacco plants, and further analysis revealed that ClMTB improved drought resistance by enhancing the reactive oxygen species (ROS) scavenging system and alleviating photosynthesis inhibition under drought conditions (He et al, 2021b). Through the identification of the HSP70 gene family and expression analysis, researchers found that HSP70 is induced when watermelon is subjected to drought stress (Wang et al, 2023a).…”

Section: Introductionmentioning

confidence: 99%

Joint transcriptomic and metabolomic analysis provides new insights into drought resistance in watermelon (Citrullus lanatus)

Chen,

Zhong,

et al. 2024

Front. Plant Sci.

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IntroductionWatermelon is an annual vine of the family Cucurbitaceae. Watermelon plants produce a fruit that people love and have important nutritional and economic value. With global warming and deterioration of the ecological environment, abiotic stresses, including drought, have become important factors that impact the yield and quality of watermelon plants. Previous research on watermelon drought resistance has included analyzing homologous genes based on known drought-responsive genes and pathways in other species.MethodsHowever, identifying key pathways and genes involved in watermelon drought resistance through high-throughput omics methods is particularly important. In this study, RNA-seq and metabolomic analysis were performed on watermelon plants at five time points (0 h, 1 h, 6 h, 12 h and 24 h) before and after drought stress.ResultsTranscriptomic analysis revealed 7829 differentially expressed genes (DEGs) at the five time points. The DEGs were grouped into five clusters using the k-means clustering algorithm. The functional category for each cluster was annotated based on the Kyoto Encyclopedia of Genes and Genomes (KEGG) database; different clusters were associated with different time points after stress. A total of 949 metabolites were divided into 10 categories, with lipids and lipid-like molecules accounting for the most metabolites. Differential expression analysis revealed 22 differentially regulated metabolites (DRMs) among the five time points. Through joint analysis of RNA-seq and metabolome data, the 6-h period was identified as the critical period for watermelon drought resistance, and the starch and sucrose metabolism, plant hormone signal transduction and photosynthesis pathways were identified as important regulatory pathways involved in watermelon drought resistance. In addition, 15 candidate genes associated with watermelon drought resistance were identified through joint RNA-seq and metabolome analysis combined with weighted correlation network analysis (WGCNA). Four of these genes encode transcription factors, including bHLH (Cla97C03G068160), MYB (Cla97C01G002440), HSP (Cla97C02G033390) and GRF (Cla97C02G042620), one key gene in the ABA pathway, SnRK2-4 (Cla97C10G186750), and the GP-2 gene (Cla97C05G105810), which is involved in the starch and sucrose metabolism pathway.DiscussionIn summary, our study provides a theoretical basis for elucidating the molecular mechanisms underlying drought resistance in watermelon plants and provides new genetic resources for the study of drought resistance in this crop.

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Overexpression of watermelon m6A methyltransferase ClMTB enhances drought tolerance in tobacco by mitigating oxidative stress and photosynthesis inhibition and modulating stress-responsive gene expression

Cited by 15 publications

References 53 publications

Comparative Genomics and Functional Studies of Putative m6A Methyltransferase (METTL) Genes in Cotton

Comparative Genomics and Functional Studies of Putative m6A Methyltransferase (METTL) Genes in Cotton

Genome-wide identification of the N6-methyladenosine regulatory genes reveals NtFIP37B increases drought resistance of tobacco (Nicotiana tabacum L.)

Joint transcriptomic and metabolomic analysis provides new insights into drought resistance in watermelon (Citrullus lanatus)

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