Overexpression of VOZ2 confers biotic stress tolerance but decreases abiotic stress resistance in Arabidopsis

Nakai, Yusuke; Fujiwara, Shigeru; Kubo, Yasuyuki; Sato, Masa H.

doi:10.4161/psb.23358

Cited by 25 publications

(35 citation statements)

References 8 publications

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“…20,21) In addition, VOZ1 and VOZ2 are involved in changing the expression of various stress-related genes and controlling both abiotic and biotic stress-responsive pathways, such as those related to freezing responses and pathogens. 22,23) Recently, it was reported that the voz1 voz2 mutant is responsive to vernalization. 24) In the present study, we further investigated VOZ function in the regulation of FLC, analyzing the responses to vernalization using voz1 voz2 mutants both in Col and FRI FLC backgrounds.…”

Section: )mentioning

confidence: 99%

VASCULAR PLANT ONE-ZINC FINGER1 and VOZ2 repress the FLOWERING LOCUS C clade members to control flowering time in Arabidopsis

Yasui

Kohchi

2014

Bioscience, Biotechnology, and Biochemistry

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Floral transition is regulated by environmental and endogenous signals. Previously, we identified VASCULAR PLANT ONE-ZINC FINGER1 (VOZ1) and VOZ2 as phytochrome B-interacting factors. VOZ1 and VOZ2 redundantly promote flowering and have pivotal roles in the downregulation of FLOWERING LOCUS C (FLC), a central repressor of flowering in Arabidopsis. Here, we showed that the late-flowering phenotypes of the voz1 voz2 mutant were suppressed by vernalization in the Columbia and FRIGIDA (FRI)-containing accessions, which indicates that the late-flowering phenotype of voz1 voz2 mutants was caused by upregulation of FLC. We also showed that the other FLC clade members, MADS AFFECTING FLOWERING (MAF) genes, were also a downstream target of VOZ1 and VOZ2 as their expression levels were also increased in the voz1 voz2 mutant. Our results suggest that the FLC clade genes integrate signals from VOZ1/VOZ2 and vernalization to regulate flowering.

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Section: )mentioning

confidence: 99%

VASCULAR PLANT ONE-ZINC FINGER1 and VOZ2 repress the FLOWERING LOCUS C clade members to control flowering time in Arabidopsis

Yasui

Kohchi

2014

Bioscience, Biotechnology, and Biochemistry

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“…Both drought and cold stress led to 26S proteasome‐mediated degradation of VOZ2 protein in plants, and voz1voz2 plants exhibit enhanced resistance to these stress conditions. Correspondingly, overexpression of VOZ2 leads to enhanced susceptibility to abiotic stresses (Nakai, Fujiwara, et al, ). Based on these observations, we speculated that BTS may play some role in mediating responses to abiotic stress conditions beyond that of iron deprivation through its interactions with VOZ1/2.…”

Section: Resultsmentioning

confidence: 99%

“…In an effort to explore potential new roles for BTS beyond iron homeostasis, we investigated interacting partners of BTS identified through Yeast‐2‐Hybrid analysis (Long et al, ). Of particular interest was VOZ2, a transcription factor involved in both abiotic and biotic stress responses and development and targeted by an as of yet unknown E3 ligase for 26S proteasome‐mediated degradation (Nakai et al, ; Nakai, Fujiwara, Kubo, & Sato, ). Here, we focus specifically on the interaction between BTS and VOZ2.…”

Section: Introductionmentioning

confidence: 99%

“…Here, we focus specifically on the interaction between BTS and VOZ2. Given the redundant functionality of VOZ2 and related protein VOZ1, we extended some of these analyses to include VOZ1 as well (Nakai, Fujiwara, et al, ; Yasui et al, ).…”

Section: Introductionmentioning

confidence: 99%

“…Although VOZ1 and VOZ2 are constitutively expressed throughout development, they are transcriptionally repressed in response to abiotic stresses such as cold and drought. Abiotic stress markers are elevated in the voz1voz2 double knockout mutant and repressed when VOZ2 is overexpressed, further indicating that VOZ can act as both a negative and positive regulator to specific stress responses (Nakai, Fujiwara, et al, ; Nakai, Nakahira, et al, ).…”

Section: Introductionmentioning

confidence: 99%

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The E3 ligase BRUTUS facilitates degradation of VOZ1/2 transcription factors

Selote

Matthiadis

Gillikin

et al. 2018

Plant Cell & Environment

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BRUTUS (BTS) is an iron binding E3 ligase that has been shown to bind to and influence the accumulation of target basic helix-loop-helix transcription factors through 26S proteasome-mediated degradation in Arabidopsis thaliana. Vascular Plant One-Zinc finger 1 (VOZ1) and Vascular plant One-Zinc finger 2 (VOZ2) are NAM, ATAF1/2 and CUC2 (NAC) domain transcription factors that negatively regulate drought and cold stress responses in plants and have previously been shown to be degraded via the 26S proteasome. However, the mechanism that initializes this degradation is unknown. Here, we show that BTS interacts with VOZ1 and VOZ2 and that the presence of the BTS RING domain is essential for these interactions. Through cell-free degradation and immunodetection analyses, we demonstrate that BTS facilitates the degradation of Vascular plant One-Zinc finger 1/2 (VOZ1/2) protein in the nucleus particularly under drought and cold stress conditions. In addition to its known role in controlling the iron-deficiency response in plants, here, we report that BTS may play a role in drought and possibly other abiotic stress responses by facilitating the degradation of transcription factors, VOZ1/2.

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VOZ1, a transcriptional repressor of DREB2C, mediates heat stress responses in Arabidopsis

Song

Lee

Kim

et al. 2018

Planta

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Under normal growth conditions, Arabidopsis VOZ1 interacts with DREB2C and acts as a transcriptional repressor by reducing DNA binding of DREB2C. Under heat stress conditions, VOZ1 is degraded by ubiquitination, and DREB2C, which is freed from VOZ1, functions as a transcription activator. To investigate the mechanism by which the DEHYDRATION-RESPONSIVE ELEMENT-BINDING FACTOR 2C (DREB2C)-dependent signaling cascade regulates heat stress (HS) responses, we performed a yeast two-hybrid screening using the DREB2C APETALA2 (AP2) DNA-binding domain as the bait against a cDNA library derived from Arabidopsis. We identified VASCULAR PLANT ONE-ZINC-FINGER 1 (VOZ1) and further verified positive VOZ1 colonies by repeating the X-α-Gal second screening and pull-down assay in vitro. Deletion analysis of VOZ1 demonstrated that the amino acid residues in its transcriptional regulatory, zinc finger and NAC domains are essential for the DREB2C-AP2 interaction. Although the HsfA3 promoter was strongly transactivated by DREB2C in Arabidopsis protoplasts, transient co-expression of VOZ1 (35S:VOZ1) with DREB2C (35S:DREB2C) in Arabidopsis protoplasts resulted in a significant decrease in the activity of GUS fused to the HsfA3 promoter (Prom :GUS), indicating that VOZ1 acts as a repressor of DREB2C. In electrophoretic mobility shift assays (EMSAs), the signal generated by binding of DREB2C to DRE gradually decreased with increasing VOZ1 level, providing evidence that the interaction of the DREB2C AP2 DNA-binding domain with DRE is blocked by VOZ1. Additionally, a voz1 voz2-2 double knockout mutant exhibited increased HS tolerance, likely due to the suppressive function of VOZs. Taken together, these results demonstrate that VOZ1 functions as a negative regulator of HS-inducible DREB2C signaling by blocking access to the AP2 DNA-binding domain of DREB2C.

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Overexpression of VOZ2 confers biotic stress tolerance but decreases abiotic stress resistance in Arabidopsis

Cited by 25 publications

References 8 publications

VASCULAR PLANT ONE-ZINC FINGER1 and VOZ2 repress the FLOWERING LOCUS C clade members to control flowering time in Arabidopsis

VASCULAR PLANT ONE-ZINC FINGER1 and VOZ2 repress the FLOWERING LOCUS C clade members to control flowering time in Arabidopsis

The E3 ligase BRUTUS facilitates degradation of VOZ1/2 transcription factors

VOZ1, a transcriptional repressor of DREB2C, mediates heat stress responses in Arabidopsis

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