Overexpression of VaWRKY12, a transcription factor from Vitis amurensis with increased nuclear localization under low temperature, enhances cold tolerance of plants

Zhang, Langlang; Zhao, Tingting; Sun, Xiaoming; Wang, Yi; Du, Can; Zhu, Zhenfei; Gichuki, Duncan Kiragu; Wang, Qingfeng; Li, Shaohua; Xin, H. P.

doi:10.1007/s11103-019-00846-6

Cited by 50 publications

(26 citation statements)

References 89 publications

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“…Furthermore, overexpression of TaWRKY008, TaWRKY122 and TaWRKY45 in T. aestivum also enhanced tolerance to extreme temperature stress [28]. Overexpression of VaWRKY12 that was localized in the nucleus inhibited the cellular damage in Arabidopsis and grapevine after cold stress [29], while overexpression of ZmWRKY106 in Zea mays showed higher activities of antioxidants under heat stress and improved vigor of transgenic plants [30].…”

Section: Function and Expression Pattern Of Wrkys Under Abiotic Stressesmentioning

confidence: 99%

Transcription Factors in Plant Stress Responses: Challenges and Potential for Sugarcane Improvement

Javed

Shabbir

Ali

et al. 2020

Plants

165

107

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Increasing vulnerability of crops to a wide range of abiotic and biotic stresses can have a marked influence on the growth and yield of major crops, especially sugarcane (Saccharum spp.). In response to various stresses, plants have evolved a variety of complex defense systems of signal perception and transduction networks. Transcription factors (TFs) that are activated by different pathways of signal transduction and can directly or indirectly combine with cis-acting elements to modulate the transcription efficiency of target genes, which play key regulators for crop genetic improvement. Over the past decade, significant progresses have been made in deciphering the role of plant TFs as key regulators of environmental responses in particular important cereal crops; however, a limited amount of studies have focused on sugarcane. This review summarizes the potential functions of major TF families, such as WRKY, NAC, MYB and AP2/ERF, in regulating gene expression in the response of plants to abiotic and biotic stresses, which provides important clues for the engineering of stress-tolerant cultivars in sugarcane.

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Section: Function and Expression Pattern Of Wrkys Under Abiotic Stressesmentioning

confidence: 99%

Transcription Factors in Plant Stress Responses: Challenges and Potential for Sugarcane Improvement

Javed

Shabbir

Ali

et al. 2020

Plants

165

107

View full text Add to dashboard Cite

show abstract

“…Overexpression of OsWRKY11 enhanced tolerance to drought and heat in transgenic rice [ 18 ]. The grapevine ( Vitis amurensis ) VaWRKY12 gene enhanced cold tolerance in transgenic grapevine calli [ 19 ]. In wild sorghum ( Sorghum propinquum ), SpWRKY controls seed shattering but is unrelated to seed shattering genes selected during domestication, as it likely arose recently [ 20 ].…”

Section: Introductionmentioning

confidence: 99%

Genome-wide Identification of WRKY transcription factor family members in sorghum (Sorghum bicolor (L.) moench)

et al. 2020

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WRKY transcription factors regulate diverse biological processes in plants, including abiotic and biotic stress responses, and constitute one of the largest transcription factor families in higher plants. Although the past decade has seen significant progress towards identifying and functionally characterizing WRKY genes in diverse species, little is known about the WRKY family in sorghum ( Sorghum bicolor (L.) moench). Here we report the comprehensive identification of 94 putative WRKY transcription factors ( Sb WRKYs). The Sb WRKYs were divided into three groups (I, II, and III), with those in group II further classified into five subgroups (IIa–IIe), based on their conserved domains and zinc finger motif types. WRKYs from the model plant Arabidopsis ( Arabidopsis thaliana ) were used for the phylogenetic analysis of all SbWRKY genes. Motif analysis showed that all Sb WRKYs contained either one or two WRKY domains and that Sb WRKYs within the same group had similar motif compositions. SbWRKY genes were located on all 10 sorghum chromosomes, and some gene clusters and two tandem duplications were detected. SbWRKY gene structure analysis showed that they contained 0–7 introns, with most SbWRKY genes consisting of two introns and three exons. Gene ontology (GO) annotation functionally categorized SbWRKYs under cellular components, molecular functions and biological processes. A cis -element analysis showed that all SbWRKYs contain at least one stress response-related cis -element. We exploited publicly available microarray datasets to analyze the expression profiles of 78 SbWRKY genes at different growth stages and in different tissues. The induction of SbWRKYs by different abiotic stresses hinted at their potential involvement in stress responses. qRT-PCR analysis revealed different expression patterns for SbWRKYs during drought stress. Functionally characterized WRKY genes in Arabidopsis and other species will provide clues for the functional characterization of putative orthologs in sorghum. Thus, the present study delivers a solid foundation for future functional studies of SbWRKY genes and their roles in the response to critical stresses such as drought.

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“…A total of 58 PpWRKY genes have been identified in the genome of Prunus persica [16]. WRKY proteins have been verified to be essential in the defense response to various biotic and abiotic stresses, including low or high temperature, mechanical damage, and pathogenic infection [17,18,19,20,21,22,23]. In addition, there is a great deal of definite evidence indicating that the regulation of plant development and metabolism also requires WRKYs.…”

Section: Introductionmentioning

confidence: 99%

Genome-Wide Analysis of Members of the WRKY Gene Family and Their Cold Stress Response in Prunus mume

Bao

Ding

Cheng

et al. 2019

Genes

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Prunus mume, which is a rosaceous arbor with very high ornamental, edible and medical values, has a distribution that is mainly restricted by low temperature. WRKY transcription factor genes play crucial roles in the growth, development, and stress responses of plants. However, the WRKY gene family has not been characterised in P. mume. There were 58 PmWRKYs identified from genome of P. mume. They were anchored onto eight link groups and categorised into three broad groups. The gene structure and motif composition were reasonably conservative in each group. Investigation of gene duplication indicated that nine and seven PmWRKYs were arranged in tandem and segmental duplications, respectively. PmWRKYs were discriminately expressed in different tissues (i.e., roots, stems, leaves, flowers and fruits) in P. mume. The 17 cold-related candidate genes were selected based on RNA-seq data. Further, to investigate the function of PmWRKYs in low temperatures, the expression patterns under artificial cold treatments were analysed. The results showed that the expression levels of the 12 PmWRKYs genes significantly and 5 genes slightly changed in stems. In particular, the expression level of PmWRKY18 was up-regulated after ABA treatment. In addition, the spatiotemporal expression patterns of 17 PmWRKYs were analysed in winter. These results indicated that 17 PmWRKYs were potential transcription factors regulating cold resistance in P. mume.

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Overexpression of VaWRKY12, a transcription factor from Vitis amurensis with increased nuclear localization under low temperature, enhances cold tolerance of plants

Cited by 50 publications

References 89 publications

Transcription Factors in Plant Stress Responses: Challenges and Potential for Sugarcane Improvement

Transcription Factors in Plant Stress Responses: Challenges and Potential for Sugarcane Improvement

Genome-wide Identification of WRKY transcription factor family members in sorghum (Sorghum bicolor (L.) moench)

Genome-Wide Analysis of Members of the WRKY Gene Family and Their Cold Stress Response in Prunus mume

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