Overexpression of vascular endothelial growth factor164 and its co-receptor neuropilin-1 in estrogen-induced rat pituitary tumors and GH3 rat pituitary tumor cells.

Localization of vascular endothelial growth factor (VEGF) receptors in normal and adenomatous pituitaries: detection of a non-endothelial function of VEGF in pituitary tumours

“…Strong overexpression of the VEGF/VEGF receptor system has been reported in rapidly growing and densely vascularized types of solid tumours (Carmeliet 2003, Ferrara 2004). In the case of the pituitary, only oestrogen-induced prolactinomas in Fischer 344 rats exhibit such tumour characteristics and over-expression of both VEGFR-2 and NRP-1 was found in this tumour type (Banerjee et al 1997(Banerjee et al , 2000. Since the characteristics of human pituitary adenomas are completely different, strong over-expression of VEGFR-2 would not fit to these slowly growing and poorly vascularized tumours.…”

“…Human adenomas are mostly less well vascularized than the normal anterior pituitary (Gorczyca & Hardy 1988, Turner et al 2000, Vidal et al 2001, De la Torre et al 2005 and no enhanced VEGF-A expression was found in human pituitary tumours, in comparison with the normal gland, with the exception of the study of McCabe et al (2002). Only in rapidly growing oestradiol-induced experimental rat prolactinomas with a vessel density higher than in the normal rat pituitary, was an over-expression of VEGF-A was observed (Banerjee et al 1997(Banerjee et al , 2000.…”

Expression and Distribution of Vascular Endothelial Growth Factor Receptor Flk-1 in the Rat Pituitary

“…An intrinsic intercellular communication network appears to be involved in the control of pituitary homeostasis, particularly with regard to gene expression and the production/secretion of anterior pituitary hormones (Arzt et al 1998(Arzt et al ,1999. VEGF is also an autocrine/paracrine molecule secreted by normal and neoplastic anterior pituitary cells (Banerjee et al 1997(Banerjee et al ,2000Lloyd et al 1999;Vidal et al 1999Vidal et al , 2000aOchoa et al 2000). Although unanswered questions remain regarding the functional signifcant of VEGF expression in the adenohypophysis, it is conceivable that VEGF plays a role both in the formation of pituitary portal vessels during fetal life and in maintenance of their differentiated state in mature animals (Ferrara et al 1992).…”

Neuropilin‐1 is differentially expressed in myoepithelial cells and vascular smooth muscle cells in preneoplastic and neoplastic human breast: A possible marker for the progression of breast cancer

“…For RT-PCR analyses, RNA was extracted from frozen biopsy samples and amplified according to the method described by Banerjee et al 13 Detection of NRP-1 mRNA was carried out using human NRP-1 gene specific primers. Amplification was carried out with parameters of 4 min at 94°C for denaturation followed by 30 cycles of 30 sec at 94°C, 30 sec at 60°C, 30 sec at 72°C, and ending with 10 min at 72°C in a DNA thermal cycler (GeneAmp PCR System 2400, CA).…”