Overexpression of VaPAT1, a GRAS transcription factor from Vitis amurensis, confers abiotic stress tolerance in Arabidopsis

Yuan, Yangyang; Fang, Linchuan; Karungo, Sospeter Karanja; Zhang, Langlang; Gao, Yingying; Li, Shaohua; Xin, H. P.

doi:10.1007/s00299-015-1910-x

Cited by 102 publications

(56 citation statements)

References 52 publications

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“…For example, Yang et al (2012) confirmed that the activities of POD, SOD and CAT increased in plants over-expressing OsMYB2 , under salt stress. However, Yuan et al (2015) reported that under cold stress, antioxidase activity was not significantly different in VaPAT1- over-expressing plants compared to WT plants. Shingote et al (2015) found POD and CAT activities fell, while SOD activity increased in transgenic plants under cold stress.…”

Section: Discussionmentioning

confidence: 95%

VaERD15, a Transcription Factor Gene Associated with Cold-Tolerance in Chinese Wild Vitis amurensis

Zhang

Zhao

et al. 2017

Front. Plant Sci.

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Early responsive to dehydration (ERD) genes can be rapidly induced to counteract abiotic stresses, such as drought, low temperatures or high salinities. Here, we report on an ERD gene (VaERD15) related to cold tolerance from Chinese wild Vitis amurensis accession ‘Heilongjiang seedling’. The full-length VaERD15 cDNA is 685 bp, including a 66 bp 5′-untranslated region (UTR), a 196 bp 3′-UTR region and a 423 bp open reading frame encoding 140 amino acids. The VaERD15 protein shares a high amino acid sequence similarity with ERD15 of Arabidopsis thaliana. In our study, VaERD15 was shown to have a nucleic localization function and a transcriptional activation function. Semi-quantitative PCR and Western blot analyses showed that VaERD15 was constitutively expressed in young leaves, stems and roots of V. amurensis accession ‘Heilongjiang seedling’ plants, and expression levels increased after low-temperature treatment. We also generated a transgenic Arabidopsis Col-0 line that over-expressed VaERD15 and carried out a cold-treatment assay. Real-time quantitative PCR (qRT-PCR) and Western blot analyses showed that as the duration of cold treatment increased, the expression of both gene and protein levels increased continuously in the transgenic plants, while almost no expression was detected in the wild type Arabidopsis. Moreover, the plants that over-expressed VaERD15 showed higher cold tolerance and accumulation of proline, soluble sugars, proteins, malondialdehyde and three antioxidases (superoxide dismutase, peroxidase, and catalase). Lower levels of relative ion leakage also occurred under cold stress. Taken together, our results indicate that the transcription factor VaERD15 was induced by cold stress and was able to enhance cold tolerance.

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Section: Discussionmentioning

confidence: 95%

VaERD15, a Transcription Factor Gene Associated with Cold-Tolerance in Chinese Wild Vitis amurensis

Zhang

Zhao

et al. 2017

Front. Plant Sci.

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“…The genes in the PAT1 branch of the GRAS family regulate diverse functions in plants. Several A. thaliana genes belong to the AtPAT1 subbranch of the GRAS family, namely AtSCL21, AtSCL5, AtSCL13, and AtSCL1 (Yuan et al, 2016). Two rice genes, OsCIGR1 and OsCIGR2, are grouped in the same sub-branch.…”

Section: Discussionmentioning

confidence: 99%

Phytochrome A signal transduction 1 and CONSTANS‐LIKE 13 coordinately orchestrate shoot branching and flowering in leafy Brassica juncea

Muntha

Zhang

Zhou

et al. 2019

Plant Biotechnology Journal

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Summary Branching is a major determinant of crop yield, and enables vigorous shoot growth and the production of a dense canopy. Phytochrome A signal transduction 1 ( PAT 1) positively regulates phytochrome A signal transduction in response to light, but its effects on branching remain unknown. In this study, we mapped PAT 1 , and revealed a previously unknown role related to branching and flowering in leafy Brassica juncea . Earlier and increased branching was observed when PAT 1 expression was down‐regulated, implying that PAT 1 negatively regulates shoot branching. Additionally, down‐regulated PAT 1 expression reversed the inhibited branching induced by far‐red light, suggesting PAT 1 is involved in the shade avoidance response. PAT 1 negatively regulated branching only after bud initiation. The observed interaction between PAT 1 and BRC 1 implied that PAT 1 influences bud outgrowth in a BRC 1‐dependent manner. Biochemical and genetic evidence indicate that PAT 1 directly interacts with CONSTANS ‐ LIKE 13 ( COL 13), which negatively regulates flowering, with the resulting PAT 1– COL 13 complex mediating shoot branching and flowering. Our findings reveal a new crosstalk modality between phytochrome signalling and flowering pathways during the regulation of shoot branching and flowering. The data presented herein may be useful for future studies involving the editing of the GRAS family transcription factor PAT 1 gene to enhance crop productivity and enable earlier harvesting.

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“…The identification of cold‐responsive genes may provide resources to improve cold tolerance in grapevine through molecular breeding. Our laboratory has identified various TF genes, such as VaCBF4 (Li et al ., ), VvICE1 (Li et al ., ), VaERF057 (Sun et al ., ), VaPAT1 (Yuan et al ., ), and VaAQUILO (Sun et al ., ), which are inducible by cold stress and that confer cold tolerance when expressed in A. thaliana or grapevine.…”

Section: Introductionmentioning

confidence: 99%

The ethylene response factor VaERF092 from Amur grape regulates the transcription factor VaWRKY33, improving cold tolerance

et al. 2019

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Summary Cold stress is a major limiting factor in grape (Vitis) productivity. In this study, we characterized a cold‐responsive ethylene response factor (ERF) transcription factor, VaERF092, from Amur grape (Vitis amurensis). VaERF092 expression was induced by both low temperatures and the ethylene precursor 1‐aminocyclopropane‐1‐carboxylate (ACC), but was suppressed by treatment with the ethylene inhibitor aminoethoxyvinylglycine (AVG) under cold conditions. Ectopic expression of VaERF092 in Arabidopsis thaliana enhanced cold tolerance. Co‐expression network analysis of V. vinifera genes indicated that WRKY33 might be a downstream target of VaERF092. This hypothesis was supported by the fact that VaWRKY33 was expressed temporally after VaERF092 expression and could also be induced by cold and ACC, and inhibited by AVG. Yeast one‐hybrid, transient β‐glucuronidase (GUS) and dual‐luciferase reporter assays provided evidence for an interaction between VaERF092 and a GCC‐box element in the VaWRKY33 promoter. In addition, heterologous overexpression of VaWRKY33 in A. thaliana resulted in enhanced cold tolerance. VaERF092‐ and VaWRKY33 overexpressing grape calli showed lower low‐temperature exothermic values than the empty vector (EV) calli, indicating enhanced tolerance to cold. Together, these results indicated that VaERF092 regulates VaWRKY33 through binding to its promoter GCC‐box, leading to enhanced cold stress tolerance.

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Overexpression of VaPAT1, a GRAS transcription factor from Vitis amurensis, confers abiotic stress tolerance in Arabidopsis

Cited by 102 publications

References 52 publications

VaERD15, a Transcription Factor Gene Associated with Cold-Tolerance in Chinese Wild Vitis amurensis

VaERD15, a Transcription Factor Gene Associated with Cold-Tolerance in Chinese Wild Vitis amurensis

Phytochrome A signal transduction 1 and CONSTANS‐LIKE 13 coordinately orchestrate shoot branching and flowering in leafy Brassica juncea

The ethylene response factor VaERF092 from Amur grape regulates the transcription factor VaWRKY33, improving cold tolerance

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