Overexpression of UHRF1 gene correlates with the major clinicopathological parameters in urinary bladder cancer

Abstract

Introduction Recently, expression of the UHRF1 gene was found to be up-regulated in numerous neoplasms, including the urinary bladder transitional cell carcinoma (TCC).Objective The aim of our study was to determine if the expression levels of UHRF1 gene correlates with the major pathological characteristics of the tumor and patients’ clinical outcome.Materials and Methods In our study, we have analyzed the tissue samples derived from group of 70 patients with histologically confirmed TCC of the urinary bladder, while normal urinary bladder mucosa obtained from 40 patients with nonmalignant diseases was used as a negative control group. Expression of UHRF1 gene in each patient sample was determined using reverse transcriptase-polymerase chain reaction.Results UHRF1 gene expression was found to be app. 2.5 times higher in samples from patients with TCC in comparison with normal epithelium derived from control group patients. Analysis show that gene expression correlates with the malignancy of the tumor. A highly significant differences were found between the expression values of samples from low and high grade TCC, as well as between the high grade and control group. UHRF1 expression was higher in patients with non-muscle invasive disease than in those with muscle invasive disease.Conclusions The result of this study indicates that UHRF1 gene expression levels correlates with the major pathological characteristics of TCC samples and with the clinical outcome of those patients. Determination of UHRF1 gene expression could have a potential to be used as a sensitive molecular marker in patients with urinary bladder cancer.

UHRF1 mediates cell migration and invasion of gastric cancer

“…In gallbladder cancer, UHRF1 depletion is associated with cell apoptosis and cell cycle arrest [ 19 ]. UHRF1 expression enhances in nasopharyngeal carcinoma tissues and acted a tumor inductor in bladder cancer [ 20 ]. In the present study, we have identified that UHRF1 acts as an oncogene in GC.…”

Epigenetic mechanism and target therapy of UHRF1 protein complex in malignancies

“…Due to overexpression in various types of cancers such as gastric cancer,85 colorectal cancer,86 hepatocellular carcinoma,87 ESCC,48,88 prostate cancer,89,90 bladder cancer,91 breast cancer,92 lung cancer,93,94 melanoma,95 medulloblastoma,96 lymphoblastic leukemia,97 and osteosarcoma 98. UHRF1 appears a potential biomarker and is promising in the target therapy of cancers.…”

The epigenetic integrator UHRF1: on the road to become a universal biomarker for cancer

“…Other studies reported similar overexpression of UHRF1 in bladder cancers and in invasive cell lines, such as 253J, T24, KU7, along with silencing of tumor suppressor genes e.g ., KISS1 and RGS2 [ 56 , 112 , 113 ]. Altogether, these studies emphasize UHRF1 as an attractive biomarker and therapeutic target for bladder cancers.…”