Overexpression of the ZmDEF1 gene increases the resistance to weevil larvae in transgenic maize seeds

Thuy, Thi Xuan; Nguyen, Tan N.; Pham, Thi Thanh Nhan; Nguyen, Huu Quan; Nguyen, Thi Hai Yen; Tân, Từ Quang; Le, Van Son; Mậu, Chu Hoàng

doi:10.1007/s11033-019-04670-5

Cited by 10 publications

(7 citation statements)

References 20 publications

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“…The ZmDEF1 gene under the control of a seed-specific promoter was also expressed in tobacco seeds [ 101 ]. The same gene was transferred to two maize cultivars, LC1 and LVN99, using immature embryos to improve weevil resistance [ 104 ].…”

Section: Defensins Of Grassesmentioning

confidence: 99%

“…The protein extracts from seeds of the transgenic plants reduced weevil α-amylase activity by 67.9–71.4% in comparison with protein extracts from nontransgenic plants [ 101 ]. Furthermore, overexpression of the ZmDEF1 gene in two Vietnamese maize cultivars (LC1 and LVN99) resulted in increased ability of the seed protein extracts to inhibit the α-amylase activity of S. zeamais larvae by 54.52‒63.09% as compared to nontransgenic plants [ 104 ].…”

Section: Defensins Of Grassesmentioning

confidence: 99%

“…Transgenic plants overexpressing the maize ZmDEF1 gene in tobacco conferred enhanced resistance to Phytophothora parasitica var. nicotiana , while overexpression of this gene in two Vietnamese maize cultivars LC1 and LVN99 resulted in an increased inhibitory activity against α-amylases of Sitophilus zeamais larvae, suggesting a role in pest defense [ 101 , 104 ]. Thus, the antimicrobial activity of defensins from grasses may have practical applications in crop protection to combat fungal and bacterial pathogens and insect pests, as well as in medicine.…”

Section: Molecular Diversity and Functions Of Grass Defensins: Thementioning

confidence: 99%

“…Of the grass defensins, three SIα1‒3 defensins of Sorghum bicolor and one defensin ZmDEF1 of Z. mays exhibit inhibitory activity against insect α-amylases, while the γ-hordothionin of H. vulgare inhibits the α-amylase from human saliva ( Figure 7 ) [ 47 , 50 , 101 , 104 ]. The residues responsible for the enzyme inhibitory activity are largely unknown.…”

Section: Molecular Diversity and Functions Of Grass Defensins: Thementioning

confidence: 99%

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Defensins of Grasses: A Systematic Review

Одинцова

Slezina

2020

Biomolecules

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The grass family (Poaceae) is one of the largest families of flowering plants, growing in all climatic zones of all continents, which includes species of exceptional economic importance. The high adaptability of grasses to adverse environmental factors implies the existence of efficient resistance mechanisms that involve the production of antimicrobial peptides (AMPs). Of plant AMPs, defensins represent one of the largest and best-studied families. Although wheat and barley seed γ-thionins were the first defensins isolated from plants, the functional characterization of grass defensins is still in its infancy. In this review, we summarize the current knowledge of the characterized defensins from cultivated and selected wild-growing grasses. For each species, isolation of defensins or production by heterologous expression, peptide structure, biological activity, and structure–function relationship are described, along with the gene expression data. We also provide our results on in silico mining of defensin-like sequences in the genomes of all described grass species and discuss their potential functions. The data presented will form the basis for elucidation of the mode of action of grass defensins and high adaptability of grasses to environmental stress and will provide novel potent molecules for practical use in medicine and agriculture.

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Section: Defensins Of Grassesmentioning

confidence: 99%

Section: Defensins Of Grassesmentioning

confidence: 99%

Section: Molecular Diversity and Functions Of Grass Defensins: Thementioning

confidence: 99%

Section: Molecular Diversity and Functions Of Grass Defensins: Thementioning

confidence: 99%

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Defensins of Grasses: A Systematic Review

Одинцова

Slezina

2020

Biomolecules

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“…PTA construct for M. separata gene silencing, maize transformation and insect bioassays For multiple gene silencing, we chose MsChi2, MsActin and MsREase (Unigene No., Cluster-3827.32653) as the target genes, and the PTA vector was constructed as described above and transformed into Agrobacterium tumefaciens strain EHA101. Maize transformation was performed on immature embryos from the inbred line maize cultivar B104 according to the literature (Vi et al, 2019;Xiang et al, 2018). The complete plantlets with healthy roots were transplanted into soil pots and the transgenic seedlings were confirmed using regular PCR with genomic DNA as template.…”

Section: Synthesis Of Pta Expression Cassette and Transient Knockdown Analysis In N Benthamianamentioning

confidence: 99%

Improvement of host‐induced gene silencing efficiency via polycistronic‐tRNA‐amiR expression for multiple target genes and characterization of RNAi mechanism in Mythimna separata

Bao

Zhang

et al. 2021

Plant Biotechnology Journal

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Host-induced gene silencing (HIGS) emerged as a new strategy for pest control. However, RNAi efficiency is reported to be low in Lepidoptera, which are composed of many important crop pests. To address this, we generated transgenic plants to develop HIGS effects in a maize pest, Mythimna separata (Lepidoptera, Noctuidae), by targeting chitinase encoding genes. More importantly, we developed an artificial microRNA (amiR) based PTA (polycistronic-tRNA-amiR) system for silencing multiple target genes. Compared with hpRNA (hairpin RNA), transgenic expression of a PTA cassette including an amiR for the gut-specific dsRNA nuclease gene MsREase, resulted in improved knockdown efficiency and caused more pronounced developmental abnormalities in recipient insects. When target gene siRNAs were analysed after HIGS and direct dsRNA/siRNA feeding, common features such as sense polarity and siRNA hotspot regions were observed, however, they differed in siRNA transitivity and major 20-24nt siRNA species. Core RNAi genes were identified in M. separata, and biochemical activities of MsAGO2, MsSID1 and MsDcr2 were confirmed by EMSA (electrophoretic mobility shift assay) and dsRNA cleavage assays, respectively. Taken together, we provide compelling evidence for the existence of the RNAi mechanism in M. separata by analysis of both siRNA signatures and RNAi machinery components, and the PTA system could potentially be useful for future RNAi control of lepidopteran pests.

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Genetic Transformation in Catharanthus roseus

Nguyen

et al. 2022

Compendium of Plant Genomes

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Overexpression of the ZmDEF1 gene increases the resistance to weevil larvae in transgenic maize seeds

Cited by 10 publications

References 20 publications

Defensins of Grasses: A Systematic Review

Defensins of Grasses: A Systematic Review

Improvement of host‐induced gene silencing efficiency via polycistronic‐tRNA‐amiR expression for multiple target genes and characterization of RNAi mechanism in Mythimna separata

Genetic Transformation in Catharanthus roseus

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