Overexpression of the Wnt5b Gene in Leiomyoma Cells: Implications for a Role of the Wnt Signaling Pathway in the Uterine Benign Tumor

Mangioni, Silvia; Viganò, Paola; Lattuada, D.; Abbiati, Annalisa; Vignali, Michele; Blasio, Anna Maria Di

doi:10.1210/jc.2005-0272

Cited by 38 publications

(27 citation statements)

References 24 publications

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“…Another association was identified among genetic variants of rs2784917, gene expression of WNT5B, and etoposide IC 50 in the YRI. WNT signaling pathway plays a key role in carcinogenesis and embryogenesis and has been found to be up-regulated in gastric, esophageal, pancreatic, breast cancer cell line, and uterine leiomyoma cells (25,26). Our study showed that the TT genotype of SNP rs2784917 was associated with higher WNT5B gene expression and greater sensitivity to etoposide.…”

Section: Discussionmentioning

confidence: 51%

A genome-wide approach to identify genetic variants that contribute to etoposide-induced cytotoxicity

Huang

Duan

Bleibel

et al. 2007

Proc. Natl. Acad. Sci. U.S.A.

179

191

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Large interindividual variance has been observed in sensitivity to drugs. To comprehensively decipher the genetic contribution to these variations in drug susceptibility, we present a genome-wide model using human lymphoblastoid cell lines from the International HapMap consortium, of which extensive genotypic information is available, to identify genetic variants that contribute to chemotherapeutic agent-induced cytotoxicity. Our model inte- HapMap ͉ pharmacogenomics ͉ toxicity ͉ whole-genome association

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Section: Discussionmentioning

confidence: 51%

A genome-wide approach to identify genetic variants that contribute to etoposide-induced cytotoxicity

Huang

Duan

Bleibel

et al. 2007

Proc. Natl. Acad. Sci. U.S.A.

179

191

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“…Leiomyomas cells in culture have high levels of both transcripts of WNT5B and of secreted frizzled related protein 1 (SFRP1), a modulator of Wnt signaling (Mangioni et al 2005). Strong SFRP1 expression under high estrogenic conditions seems to contribute to the development of human uterine leiomyomas through the antiapoptotic effect of SFRP1 (Fukuhara et al 2002).…”

Section: Wnt Signalling Pathwaymentioning

confidence: 99%

Gene and protein expression in the myometrium in pregnancy and labor

Breuiller-Fouché¹,

Germain²

2006

Reproduction

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Microarray technologies widen our comprehension of the major structural and metabolic transformations which affect the myometrium from the very beginning of pregnancy until parturition. The results are coherent with the mass of information which was accumulated previously, primarily on the basis of studies of selected critical factors. They highlight the activation of precise signaling pathways, some of which may have been previously under evaluated. The remodelling and maturation processes that the myometrium undergoes in pregnancy appear clearly as phenomena which last during the full course of gestation. Comparatively, the onset of labor is perhaps the phenomenon which remains the least well described by these methods of analysis. Nevertheless, genomic studies constitute a necessary first step of orientation and help establishing new links between the generic signaling pathways that are activated during the normal or pathological gestation. These studies also represent an indicative step that will have to be paralleled, in the future, with the results of the systematic proteomic analysis of the myometrium.

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“…The molecular causes of the disease remain unknown [1][2][3][4][5][11][12][13][14] . A variety of techniques, including differential hybridization, differential display PCR, representational difference analysis, suppression subtractive hybridization (SSH), and gene array, have been used to identify the genes whose expression is selectively altered in uterine leiomyoma [1][2][3][12][13][14] .…”

Section: Discussionmentioning

confidence: 99%

“…So it is very important to explore the molecular mechanism of the cause of uterine leiomyoma at protein level by studying the differential proteins between uterine leiomyoma and normal myometrium. Previous studies examining changes in protein expression in uterine leiomyoma have mainly targeted specific proteins [4,15] . To our knowledge, there is no report in which two-dimensional electrophoresis was employed to study global protein changes in uterine leiomyoma.…”

Section: Discussionmentioning

confidence: 99%

“…Uterine leiomyomas are the most common tumor in the female genital tract, but their etiology and the molecular basis of their development and growth are unclear [1][2][3][4][5] . So it is very important to explore the molecular mechanism of the cause of uterine leiomyoma at protein level by studying the differential proteins between uterine leiomyoma and normal myometrium.…”

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confidence: 99%

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Identification of differential proteins in uterine leiomyoma by two-dimensional electrophoresis

Zhu

et al. 2006

Chin. J. Cancer Res.

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Objective: To establish and optimize the two-demensional electrophoresis maps of uterine leiomyoma and to study the difference of global protein patterns between uterine leiomyoma and normal myometrium. Methods: Using Two-dimensional electrophoresis followed by computer-assisted image analysis, the differential proteins between uterine leiomyoma and normal myometrium were compared.Results: The well-resolved and reproducible two-dimensional gel electrophoresis patterns of uterine leiomyoma and normal myometrium were established. Totally 1085±108 and 1103±151 protein spots were obtained by using the pH 4-7 IPG strips in uterine leiomyoma and normal myometrium map, respectively, of which 7 spots increased and 15 spots decreased in quantity in uterine leiomyoma compared with normal myometrium. Conclusion: The differentially expressed proteins are useful for studying the mechanism of the cause of uterine leiomyoma.

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Overexpression of the Wnt5b Gene in Leiomyoma Cells: Implications for a Role of the Wnt Signaling Pathway in the Uterine Benign Tumor

Abstract: Specific Wnt signaling genes are overexpressed in leiomyoma cells. Moreover, in these cells, the regulation of Wnt5b expression by retinoids appears to be attenuated.

Cited by 38 publications

References 24 publications

A genome-wide approach to identify genetic variants that contribute to etoposide-induced cytotoxicity

A genome-wide approach to identify genetic variants that contribute to etoposide-induced cytotoxicity

Gene and protein expression in the myometrium in pregnancy and labor

Identification of differential proteins in uterine leiomyoma by two-dimensional electrophoresis

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