Overexpression of the vesicular acetylcholine transporter disrupts cognitive performance and causes age-dependent locomotion decline in Drosophila

Showell, Shardae; Martinez, Yessica; Gondolfo, Sophia; Boppana, Sridhar; Lawal, Hakeem O.

doi:10.1016/j.mcn.2020.103483

Cited by 15 publications

(11 citation statements)

References 44 publications

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“…It is thought that the abundance of food induces an increase in acetylcholine, which is detected by GAR-3 in the pharyngeal muscles, so when this receptor is lost, the frequency of contraction of the pharyngeal muscle decreases and causes a phenomenon similar to that of occurs with dietary restriction (Lucanic et al 2016 ). This is also similar to what happens with DOP-3 (See section “Dopamine receptors ”) and the vesicular acetylcholine transporter (VAChT) in Drosophila (Showell et al 2020 ).…”

Section: Gpcr Influencing Lifespansupporting

confidence: 74%

G protein-coupled receptors that influence lifespan of human and animal models

Lagunas‐Rangel

2021

Biogerontology

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Humanity has always sought to live longer and for this, multiple strategies have been tried with varying results. In this sense, G protein-coupled receptors (GPCRs) may be a good option to try to prolong our life while maintaining good health since they have a substantial participation in a wide variety of processes of human pathophysiology and are one of the main therapeutic targets. In this way, we present the analysis of a series of GPCRs whose activity has been shown to affect the lifespan of animal and human models, and in which we put a special interest in describing the molecular mechanisms involved. Our compilation of data revealed that the mechanisms most involved in the role of GPCRs in lifespan are those that mimic dietary restriction, those related to insulin signaling and the AMPK and TOR pathways, and those that alter oxidative homeostasis and severe and/or chronic inflammation. We also discuss the possibility of using agonist or antagonist drugs, depending on the beneficial or harmful effects of each GPCR, in order to prolong people's lifespan and healthspan.

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Section: Gpcr Influencing Lifespansupporting

confidence: 74%

G protein-coupled receptors that influence lifespan of human and animal models

Lagunas‐Rangel

2021

Biogerontology

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“…The vAChT (see Boppana et al 2017 ; Deshpande et al 2020 for reviews) not only facilitates quantal transmitter packing (Song et al 1997 ), but in Drosophila also plays a role in glial differentiation (Soustelle et al 2002 ), cognitive performance and locomotion (Showell et al 2020 ). Given the homologous nature of insect nervous systems (see Meier and Reichert 1991 ; Meier et al 1991 ; Boyan and Ball 1993 ; Casares and Mann 1998 ), we expected to find the vAChT in the developing locust antennal nervous system as well.…”

Section: Discussionmentioning

confidence: 99%

“…Cell types not involved in this study are the thecogen (Th), tormogen (To) and trichogen (Tr) cells. Other abbreviations: BL basal lamina, Cu outer cuticle (color figure online) and locomotion (Showell et al 2020). Given the homologous nature of insect nervous systems (see Boyan and Ball 1993;Casares and Mann 1998), we expected to find the vAChT in the developing locust antennal nervous system as well.…”

Section: The Vesicular Acetylcholine Transporter (Vacht)mentioning

confidence: 99%

“…Acetylcholine is a ubiquitous neuromodulator with extensive roles in insect physiology and behavior (Heinrich et al 1997 ; Kunst et al 2011 ; Boppana et al 2017 ; Deshpande et al 2020 ; Showell et al 2020 ). In the fly, for example, most chemosensory, olfactory, chordotonal, and auditory primary sensory neurons are cholinergic (Salvaterra and Kitamoto 2001 ).…”

Section: Introductionmentioning

confidence: 99%

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Evidence for the cholinergic markers ChAT and vAChT in sensory cells of the developing antennal nervous system of the desert locust Schistocerca gregaria

Ehrhardt

Boyan

2020

Invert Neurosci

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Sensory and motor systems in insects with hemimetabolous development must be ready to mediate adaptive behavior directly on hatching from the egg. For the desert locust S. gregaria, cholinergic transmission from antennal sensillae to olfactory or mechanosensory centers in the brain requires that choline acetyltransferase (ChAT) and the vesicular acetylcholine transporter (vAChT) already be present in sensory cells in the first instar. In this study, we used immunolabeling to demonstrate that ChAT and vAChT are both expressed in sensory cells from identifiable sensilla types in the immature antennal nervous system. We observed ChAT expression in dendrites, neurites and somata of putative basiconic-type sensillae at the first instar stage. We also detected vAChT in the sensory axons of these sensillae in a major antennal nerve tract. We then examined whether evidence for cholinergic transmission is present during embryogenesis. Immunolabeling confirms that vAChT is expressed in somata typical of campaniform sensillae, as well as in small sensory cell clusters typically associated with either a large basiconic or coeloconic sensilla, at 99% of embryogenesis. The vAChT is also expressed in the somata of these sensilla types in multiple antennal regions at 90% of embryogenesis, but not at earlier (70%) embryonic stages. Neuromodulators are known to appear late in embryogenesis in neurons of the locust central complex, and the cholinergic system of the antenna may also only reach maturity shortly before hatching.

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“…The choline transporter on the membranes of presynaptic neurons reabsorbs choline in the synaptic cleft, and the choline transporter in neuronal vesicles can enrich the cytoplasmic ACh in vesicles. The vesicular acetylcholine transporter does not only facilitate quantal transmitter packing, but in Drosophila also plays a role in glial differentiation, cognitive performance, and locomotion [12] , [59] . ACh relies on AchR to play its part.…”

Section: Introductionmentioning

confidence: 99%