Overexpression of the tyrosine decarboxylase gene MdTyDC confers salt tolerance in apple

Wang, Yanpeng; Gao, Tengteng; Zhang, Zhijun; Yuan, Xiao; Chen, Qi; Zheng, Jiangzhu; Chen, Shuaiyin; Ma, Fengwang; Li, Chao

doi:10.1016/j.envexpbot.2020.104244

Cited by 25 publications

(10 citation statements)

References 47 publications

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“…Therefore, we measured dopamine content in leaves and roots of apple plants after 5 days of alkaline stress treatment. Consistent with the study of Wang et al (2020) , dopamine content increased when MdTyDc was overexpressed in apples ( Figures 1D,E and Supplementary Figure S1 ). In addition, dopamine content in OE plants under alkaline stress was higher than that under normal conditions.…”

Section: Discussionsupporting

confidence: 89%

“…Tyrosine decarboxylase (EC 4.1.1.25) is a 5’-pyridoxal phosphate (PLP)-dependent decarboxylase. Many studies have shown that it plays an active role in plant growth and development ( Kang et al, 2007 ), secondary metabolite synthesis ( Lan et al, 2013 ), and resistance to biotic and abiotic stresses ( Landtag et al, 2002 ; Wang et al, 2020 ). Tyrosine is decarboxylated to tyramine by TYDC and then hydroxylated to dopamine, which is involved in plant response to salt stress ( Li et al, 2015 ; Gao et al, 2020a ), drought stress ( Gao et al, 2020b ), and low N stress ( Liu et al, 2020 ).…”

Section: Discussionmentioning

confidence: 99%

“…WT and transgenic GL-3 ( Malus domestica cv. Gala) plants were obtained from the previous research in our laboratory ( Wang et al, 2020 ). After subculturing and rooting, the apple plants were transplanted to plastic pots (12 × 12 cm) filled with soil/perlite/vermiculite (4:1:1, v:v:v) and placed in a growth chamber.…”

Section: Methodsmentioning

confidence: 99%

“…Previously, we found that dopamine content was increased by the overexpression of MdTyDc in apple seedlings and calli, and apple salt tolerance was improved by its overexpression in apple plants ( Wang et al, 2020 ). Moreover, previous studies in our lab showed that the expression of N-metabolism-related genes in apple seedlings was up-regulated by exogenous dopamine under alkaline stress, permitting better adaptation to this stress ( Jiao et al, 2019 ).…”

Section: Introductionmentioning

confidence: 98%

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MdTyDc Overexpression Improves Alkalinity Tolerance in Malus domestica

et al. 2021

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Tyrosine is decarboxylated to tyramine by TYDC (Tyrosine decarboxylase) and then hydroxylated to dopamine, which is involved in plant response to abiotic stress. However, little is known about the function of MdTyDc in response to alkaline stress in plants. In our study, it was found that the expression of MdTyDc was induced by alkaline stress. Therefore, the apple plants overexpressing MdTyDc was treated with alkali stress, and we found that MdTyDc played an important role in apple plants’ resistance to alkali stress. Our results showed that the restriction on the growth, the decrease of membrane permeability and the accumulation of Na+ were alleviated to various degrees in MdTyDc transgenic plants under alkali stress. In addition, overexpression of MdTyDc enhanced the root activity and photosynthetic capacity, and improved the enzyme activity related to N metabolism, thus promoting N absorption. It is noteworthy that the dopamine content of these three transgenic lines is significantly higher than that of WT. In summary, these findings indicated that MdTyDc may enhance alkaline tolerance of apples by mediating dopamine content, mainly by maintaining high photosynthetic capacity, normal ion homeostasis and strong nitrogen absorption capacity.

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Section: Discussionsupporting

confidence: 89%

Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 98%

See 2 more Smart Citations

MdTyDc Overexpression Improves Alkalinity Tolerance in Malus domestica

et al. 2021

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“…In Northeast China, the grassland alkalization rate has exceeded 70% indicating the seriousness of soil alkalization (Yang et al, 2008 ) and necessitates more attention. However, to date, most research studies have only focused on NaCl and/or Na 2 SO 4 (Lin et al, 2017 ; Jia X. et al, 2019 ; Xiao et al, 2019 ; Chen et al, 2020 ; Wang et al, 2020 ). Asensi-Fabado et al ( 2015 ) reported that salt stress interferes with the normal physiological metabolism of plants, leading to a lack of cellular energy and oxidative stress.…”

Section: Introductionmentioning

confidence: 99%

The Alkali Tolerance of Broomcorn Millet (Panicum miliaceum L.) at the Germination and Seedling Stage: The Case of 296 Broomcorn Millet Genotypes

Liang

et al. 2021

Front. Plant Sci.

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Broomcorn millet (BM), one of the earliest domesticated cereal crops originating in northern China, can tolerate extreme conditions, such as drought and high temperatures, which are prevalent in saline-alkali, arid, and barren landscapes. However, its adaptive mechanism to alkali stress is yet to be comprehensively understood. In this study, 80 and 40 mM standard alkali stress concentrations were used to, respectively, evaluate the alkali tolerance at the germination and seedling stages of 296 BM genotypes. Principal component analysis (PCA), Pearson's correlation analysis, and F-value comprehensive analysis were performed on the germination parameters (germination potential, germination index, germination rate, vigor index, root length/weight, sprout length/weight, and alkali damage rate). Based on their respective F-values, the BM genotypes were divided into five categories ranging from highly alkali resistant to alkali sensitive. To study the response of seedlings to alkaline stress, we investigated the phenotypic parameters (plant height, green leaf area, biomass, and root structure) of 111 genotypes from the above five categories. Combining the parameters of alkali tolerance at the germination and seedling stages, these 111 genotypes were further subdivided into three groups with different alkali tolerances. Variations in physiological responses of the different alkali-tolerant genotypes were further investigated for antioxidant enzyme activity, soluble substances, malondialdehyde (MDA) content, electrolyte leakage rate, and leaf structure. Compared with alkali-sensitive genotypes, alkali-tolerant genotypes had high antioxidant enzyme activity and soluble osmolyte content, low MDA content and electrolyte leakage rate, and a more complete stomata structure. Taken together, this study provides a comprehensive and reliable method for evaluating alkali tolerance and will contribute to the improvement and restoration of saline-alkaline soils by BM.

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