Overexpression of the RIXI xylanase inhibitor improves disease resistance to the fungal pathogen, Magnaporthe oryzae, in rice

Hou, Chunxiao; Lv, Ting; Zhan, Yihua; Peng, Yonggang; Huang, Yingying; Jiang, Dean; Weng, Xiao-Yan

doi:10.1007/s11240-014-0590-5

Cited by 22 publications

(15 citation statements)

References 46 publications

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“…Osxip expression in transgenic plants was mainly observed in the shoot and root tissues after the exposure of two-week-old seedlings of rice to Nilaparvata lugens (Sun et al, 2018). Some researchers suggest that transgenic plants with over-expression of XIP may be more resistant to pathogens than wild-type plants (Hou et al, 2014;Tundo et al, 2015;Zhan et al, 2017a).…”

Section: Xylanase Inhibitor Protein (Xip)mentioning

confidence: 99%

Microbial xylanases and their inhibition by specific proteins in cereals

Chmelová

Škulcová

Ondrejovič

2019

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Arabinoxylans (AXs) belong to the components of plant cells which are mainly degraded by microbial xylanases during colonization of grain by phytopathogens. For the defence, cereals contain proteinaceous xylanase inhibitors (XIs), namely xylanase inhibitor protein (XIP), Triticum aestivum xylanase inhibitor (TAXI) and thaumatin-like xylanase inhibitor (TLXI). Their presence in cereals in high levels can be a serious problem in different industrial applications. XIs regulate AX hydrolysis and participate in plant defence mechanisms. XIs have various specificity against microbial xylanases from the glycoside hydrolase (GH) families of GH10 and GH11. Therefore, this review brings new information about the function of XIs as defence responses to pathogen infection of plants and as a problem in plant material processing in different industrial applications.

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Section: Xylanase Inhibitor Protein (Xip)mentioning

confidence: 99%

Microbial xylanases and their inhibition by specific proteins in cereals

Chmelová

Škulcová

Ondrejovič

2019

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“…Subsequently XIP‐type (xylanase inhibiting protein) and TLXI‐type (thaumatin‐like xylanase inhibitor) inhibitors were identified in plants (Juge et al ., ; Fierens et al ., ). As the inhibitors only act on exogenous xylanases and not on plant endogenous ones, they are believed to be important components of plant defense system for phytopathogen invasion (Beliën et al ., ; Hou et al ., ). The interactions between various xylanases and inhibitors are complicated.…”

Section: Introductionmentioning

confidence: 97%

Characteristics of a XIP‐resistant xylanase from Neocallimastix sp. GMLF1 and its advantage in barley malt saccharification

Zhu

Xie

et al. 2019

Int J of Food Sci Tech

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The well-studied xylanase inhibitor protein (XIP-I) regularly inhibits fungi-derived xylanases, yet some fungal xylanases are not inhibited by XIP-I. Based on the sequence alignment with the known XIP-I resistant NpXyn11A from Neocallimastix patriciarum, a new annotated xylanase from ruminal fungus Neocallimastix sp. GMLF1 was found, noted as Xyn1B, which shared 77.8% of sequence identity with NpXyn11A and was proved to be resistant to XIP-I. To evaluate the performance of a XIP resistant xylanase used in barley malt saccharification, a XIP-I sensitive xylanase ThXyn1 from Trichoderma harzianum was chosen as a control. The barley malt saccharification experiment was carried out on the condition with or without extra XIP-I added. The results showed that Xyn1B displayed only slight difference in presence and absence of added XIP-I, with the difference of xylose released (DX) and the difference of mash clarity (DA) being 0.17 g L À1 (P > 0.05) and 0.007 (P > 0.05), respectively; while those for ThXyn1 group reached 0.96 g L À1 (P < 0.01) and 0.095 (P < 0.05), indicating that XIP-I did not adversely affect Xyn1B's function, but did affect ThXyn1's function. Our work for the first time suggested that a xylanase with resistance to xylanase inhibitor proteins might have an advantage in barley malt saccharification over an inhibitor sensitive xylanase.

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“…XIP was assumed to play an important role in plant defense because it can inhibit the cell wall degrading enzymes in pathogens and prevent the necrotic activity of a xylanase (Lin and others ; Hou and others ; Moscetti and others ). However, in xylanase application areas, the inhibitors existing in the grain may compromise the activity of added xylanase.…”

Section: Introductionmentioning

confidence: 99%

Molecular Cloning and Characterizations of Xylanase Inhibitor Protein from Wheat (Triticum Aestivum)

Liu

Zhang

Wei

et al. 2017

Journal of Food Science

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Xylanase inhibitor proteins (XIPs) were regarded to inhibit the activity of xylanases during baking and gluten-starch separation processes. To avoid the inhibition to xylanases, it is necessary to define the conditions under which the inhibition takes place. In this study, we cloned the XIP gene from 2 different variety of Triticum aestivum, that is, Zhengmai 9023 and Zhengmai 366, and investigated the properties of XIP protein expressed by Pichia pastoris. The results showed that the 2 XIP genes (xip-9023 and xip-366) were highly homologous with only 3 nucleotide differences. XIP-9023 showed the optimal inhibition pH and temperature were 7 °C and 40 °C, respectively. Inhibition of xylanase by XIP-9023 reached the maximum in 40 min. At 50% inhibition of xylanase, the molar ratio of inhibitor: xylanase was 26:1. XIP-9023 was active to various fungal xylanases tested as well as to a bacterial xylanase produced by Paenibacillus sp. isolated from cow rumen.

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Overexpression of the RIXI xylanase inhibitor improves disease resistance to the fungal pathogen, Magnaporthe oryzae, in rice

Cited by 22 publications

References 46 publications

Microbial xylanases and their inhibition by specific proteins in cereals

Microbial xylanases and their inhibition by specific proteins in cereals

Characteristics of a XIP‐resistant xylanase from Neocallimastix sp. GMLF1 and its advantage in barley malt saccharification

Molecular Cloning and Characterizations of Xylanase Inhibitor Protein from Wheat (Triticum Aestivum)

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