Overexpression of the r<i>FCA</i> RNA Recognition Motif Affects Morphologies Modifications in Rice (<i>Oryza sativa</i> L.)

Fang, Hong; Attia, Kotb A.; Wang, Chun; Li, Kegui; He, Guangming; Su, Wei; Zhang, Qinghua; Qian, Xianghong; Yang, Jinshui

doi:10.1007/s10540-007-9047-y

Cited by 17 publications

(8 citation statements)

References 27 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…It has been reported that when each of the two RRMs of the homologous gene of AtFCA in Oryza sativa (r FCA ) were introduced into rice, the flowering time was delayed, and both the seed size and cell size of the transgenic plants increased (Hong et al ). Since the RRM domains of OsRRMh and rFCA are similar (42% similarity, 27% identity), we generated plants overexpressing RRM1 and RRM2 individually and/or all of them together under the control of the constitutive 35S promoter to test the unique functions of each RRM domain.…”

Section: Resultsmentioning

confidence: 99%

OsRRMh, a Spen‐Like Gene, Plays an Important Role During the Vegetative to Reproductive Transition in Rice

Liu

Cai

2013

JIPB

View full text Add to dashboard Cite

OsRRMh, a homologue of OsRRM, encodes a Spen‐like protein, and is composed of two N‐terminal RNA recognition motifs (RRM) and one C‐terminal Spen paralogue and an orthologue C‐terminal domain (SPOC). The gene has been found to be constitutively expressed in the root, stem, leaf, spikelet, and immature seed, and alternative splicing patterns were confirmed in different tissues, which may indicate diverse functions for OsRRMh. The OsRRMh dsRNAi lines exhibited late‐flowering and a larger panicle phenotype. When full‐length OsRRMh and/or its SPOC domain were overexpressed, the fertility rate and number of spikelets per panicle were both markedly reduced. Also, overexpression of OsRRMh in the Arabidopsis fpa mutant did not restore the normal flowering time, and it delayed flowering in Col plants. Therefore, we propose that OsRRMh may confer one of its functions in the vegetative‐to‐reproductive transition in rice (Oryza sativa L. subsp. japonica cv. Zhonghua No. 11 (ZH11)).

show abstract

Section: Resultsmentioning

confidence: 99%

OsRRMh, a Spen‐Like Gene, Plays an Important Role During the Vegetative to Reproductive Transition in Rice

Liu

Cai

2013

JIPB

View full text Add to dashboard Cite

show abstract

“…Genotypes in cluster 1 varied before 1997 and conserved thereafter; genotypes in cluster 2 varied before 2004 and conserved thereafter; genotypes in cluster 3 conserved until 2005 and varied thereafter, and genotypes in cluster 4 varied all through the years from 1948 to 2011. To reveal selection preference underlying these patterns in different breeding periods, we checked reported genes on them29 and found that before 1997, genes related to flowering and sterility were preferentially selected, including OsMSH5 31, rFCA 32. From 1997 to 2004, genes related to blast resistance, including Pib 33, were preferentially selected.…”

Section: Resultsmentioning

confidence: 99%

Genome-wide study of an elite rice pedigree reveals a complex history of genetic architecture for breeding improvement

Chen

Lin

Zhou

et al. 2017

Sci Rep

View full text Add to dashboard Cite

Improving breeding has been widely utilized in crop breeding and contributed to yield and quality improvement, yet few researches have been done to analyze genetic architecture underlying breeding improvement comprehensively. Here, we collected genotype and phenotype data of 99 cultivars from the complete pedigree including Huanghuazhan, an elite, high-quality, conventional indica rice that has been grown over 4.5 million hectares in southern China and from which more than 20 excellent cultivars have been derived. We identified 1,313 selective sweeps (SSWs) revealing four stage-specific selection patterns corresponding to improvement preference during 65 years, and 1113 conserved Huanghuazhan traceable blocks (cHTBs) introduced from different donors and conserved in >3 breeding generations were the core genomic regions for superior performance of Huanghuazhan. Based on 151 quantitative trait loci (QTLs) identified for 13 improved traits in the pedigree, we reproduced their improvement process in silico, highlighting improving breeding works well for traits controlled by major/major + minor effect QTLs, but was inefficient for traits controlled by QTLs with complex interactions or explaining low levels of phenotypic variation. These results indicate long-term breeding improvement is efficient to construct superior genetic architecture for elite performance, yet molecular breeding with designed genotype of QTLs can facilitate complex traits improvement.

show abstract

“…The gene LOC_Os12g01190.1 codes for a general negative regulator of transcription-like protein, which are described in both rice and Arabidopsis and contain a RNA recognition motif (Lorkovic and Barta 2002;Hong et al 2007). Expression of this gene was highly down regulated in the tolerant variety CSR 27, slightly down regulated in MI 48 but up regulated in VSR 156 and CSR 11 (Fig.…”

Section: Resultsmentioning

confidence: 99%

Differentially expressed genes in sensitive and tolerant rice varieties in response to salt-stress

Pandit

Rai

Sharma

et al. 2011

J. Plant Biochem. Biotechnol.

View full text Add to dashboard Cite

Soil salinity is a major constraint to rice production worldwide; hence intensive efforts are on to identify genes that make rice varieties tolerant to the salt stress. In this study, four rice varieties differing in tolerance to salinity were studied for differential gene expression through microarray analysis of RNA extracted from rice seedlings grown under control and salt stress conditions. Overall 1,696 gene probes in the tolerant and 2,220 gene probes in the sensitive varieties showed significant differential expression under salt stress as compared to control plants, but only 72 of these were common between the tolerant and susceptible variety groups. Fifty highest responsive genes in the tolerant and sensitive variety groups included some of the well known salt responsive genes, e. g. basic proline-rich protein, zinc finger protein and late embryogenesis abundant protein. We identified 6 genes that were commonly differentially expressed under salt stress in all the four varieties studied and hence could be the nodal points for transcriptional regulation under salt stress. These included genes for FACT complex subunit SSRP1-A, ORM1, abscisic stress ripening protein, photosystem II 10 kDa protein, negative transcription regulator-like protein and purple acid phosphatase. Expression patterns of the 6 genes were similar in the four genotypes except that all of these were down regulated in the salt tolerant variety CSR 27 under salt stress, while one gene was uniquely over expressed in highly salt sensitive variety VSR 156. These lists may contain the probable candidate genes for salt tolerance.

show abstract

Overexpression of the rFCA RNA Recognition Motif Affects Morphologies Modifications in Rice (Oryza sativa L.)

Cited by 17 publications

References 27 publications

OsRRMh, a Spen‐Like Gene, Plays an Important Role During the Vegetative to Reproductive Transition in Rice

OsRRMh, a Spen‐Like Gene, Plays an Important Role During the Vegetative to Reproductive Transition in Rice

Genome-wide study of an elite rice pedigree reveals a complex history of genetic architecture for breeding improvement

Differentially expressed genes in sensitive and tolerant rice varieties in response to salt-stress

Contact Info

Product

Resources

About