Overexpression of the protease inhibitor BoCPI-1 in broccoli delays chlorophyll loss after harvest and causes down-regulation of cysteine protease gene expression

Eason, Jocelyn R.; West, Phillip; Brummell, David A.; Watson, Lyn M.; Somerfield, Sheryl D.; McLachlan, Andrew

doi:10.1016/j.postharvbio.2014.06.006

Cited by 20 publications

(11 citation statements)

References 56 publications

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“…Our phylogenetic analysis clearly demonstrated that this gene is closely related to AtCYS-1, HvCPI-3, and OC-III within Cluster A of the four cystatin clusters (Tan et al 2014). Using a biochemical analysis with recombinant protein, we found that the proteinase activity of papain was inhibited in a concentration-dependent manner, similar to earlier research with cystatins isolated from Arabidopsis (Zhang et al 2008), barley (Gaddour et al 2001), and broccoli (Eason et al 2014). Under various abiotic stresses, MpCYS5 showed different expression patterns.…”

Section: Discussionsupporting

confidence: 88%

A Phytocystatin Gene from Malus prunifolia (Willd.) Borkh., MpCYS5, Confers Salt Stress Tolerance and Functions in Endoplasmic Reticulum Stress Response in Arabidopsis

et al. 2015

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Cystatins, or phytocystatins (PhyCys), comprise a family of plant-specific inhibitors of cysteine proteinases. They are thought to help regulate endogenous processes and protect plants against biotic or abiotic stresses, such as heat, salinity, cold, water deficit, chilling, and abscisic acid (ABA) treatment. We isolated and identified a novel cystatin gene from Malus prunifolia, MpCYS5. Its expression was typically induced by salt stress treatment; ectopic expression in Arabidopsis enhanced salt tolerance. Physiological parameters confirmed this phenotype, with the transgenics having remarkably lower electrolyte leakage (EL) values, higher chlorophyll concentrations, and lower levels of malondialdehyde (MDA) upon salt treatment. In addition, the accumulation of reactive oxygen species was markedly regulated by MpCYS5 under stress conditions, as shown by fluctuations in the concentrations of hydrogen peroxide and superoxide radicals and the activities of antioxidant enzymes. We also noted that this gene modulated tunicamycin (TM)-induced endoplasmic reticulum (ER) stress tolerance and functioned in the unfolded protein response (UPR)-signaling pathway in Arabidopsis. This was confirmed by the expression of eight ER stress-responsive genes. All marker genes examined were strongly induced in the wild type, while most of them maintained relatively stable over time in the transgenics. These results demonstrated that ectopic expression of a cystatin gene is associated with salttolerant and TM-tolerant phenotypes. Therefore, the discovery of MpCYS5 from M. prunifolia might establish a molecular link between the ER stress response and salt tolerance in plants.

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Section: Discussionsupporting

confidence: 88%

A Phytocystatin Gene from Malus prunifolia (Willd.) Borkh., MpCYS5, Confers Salt Stress Tolerance and Functions in Endoplasmic Reticulum Stress Response in Arabidopsis

et al. 2015

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“…Among others, treatments with gaseous compounds (e.g. nitric oxide (NO), ozone, hydrogen sulphide), soluble substances with antioxidant properties, chlorine and calcium-based solutions, hot water, UV radiation, high pressure, modulations of light quality and photoperiod and, genetic manipulations are shown to suppress the wound-induced browning, delay senescence, stimulate the expression of defence genes or downregulate stress- and senescence-associated genes (Coupe et al 2003; Rico et al 2006; Eason et al 2014; Li et al 2014; Mahajan et al 2014; Iakimova and Woltering 2015; Woltering and Seifu 2015). Storage under modified (MA) or controlled atmosphere (CA) with low O 2 (< 3%) and increased CO 2 levels (up to 10–15%) is another technology for preventing the occurrence of browning syndrome and premature senescence.…”

Section: Introductionmentioning

confidence: 99%

“…Fluids from dying cells may leak into the intercellular spaces causing macroscopic signs of deterioration (e.g. brown, sunken or water soaked lesions, scald and tissue dismantlement) (Cantwell and Suslow 2002; Coupe et al 2003; Fernández-Trujillo and Martínez 2006; Saltveit and Choi 2007; Hurr et al 2010; Woltering and Iakimova 2010; Eason et al 2014; Iakimova and Woltering 2015; Cantre et al 2017). The understanding of the role of PCD in postharvest disorders is, however, still in its infancy.…”

Section: Introductionmentioning

confidence: 99%

The wound response in fresh-cut lettuce involves programmed cell death events

Iakimova

Woltering

2018

Protoplasma

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In this work, the involvement of programmed cell death (PCD) in the wound-induced postharvest browning disorder and senescence in butterhead lettuce (Lactuca sativa L.) fresh-cuts was studied. At the wounded (cut, bruised) sites, rapid browning, loss of chlorophyll and massive cell death, accompanied with accumulation of reactive oxygen species and increased electrolyte leakage occurred in a narrow strip of tissue adjacent the injury. The dead cell morphology (protoplast and nuclei shrinkage) together with the biochemical and physiological changes resembled necrotic PCD type. With a slight delay post-wounding, senescence associated with similar cell death features was initiated in distant non-wounded sites. In addition to necrotic PCD, both in wounded and senescing tissue, the appearance of empty cell corpses was observed, indicating that part of the cells might undergo vacuolar PCD (self-digestion of cellular content after vacuole collapse). The wounding-induced local cell death at the primary site of damage suggested that PCD may serve as a mechanism to seal-off the wound by building a physical barrier of dead cells. However, the cell death at sites remote from the wound suggests the distribution of long-distance senescence-inducing wound messengers. Trichomes in unwounded tissue often were the first to show H2O2 accumulation and dead cells; thereafter, the elevated H2O2 and cell death appeared in connecting cells and senescence progressed over larger areas. This suggests that trichomes may contribute to mediating the wound signalling leading to subsequent senescence. Our findings demonstrate that PCD is an integral part of the wound syndrome in fresh-cut lettuce.

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“…BoCPI-1 significantly contributed to a delay in the yellowing of broccoli florets than did the suppression of BoCP5 (Eason et al, 2014). Many cystatins are strongly expressed upon exposure to abiotic stresses and the implication of the C1A CysProt-cystatin system has been reviewed (Martinez and Diaz, 2008;Martinez et al, 2012;DiazMendoza et al, 2014;Kidric et al, 2014).…”

Section: Discussionmentioning

confidence: 99%

“…Its ectopic expression in tobacco, soybean and Arabidopsis presumed a protective role towards the photosynthetic machinery during both natural and stress-induced senescence (Prins et al, 2008;Quain et al, 2014). Eason et al (2014) characterized the role of a protease inhibitor (BoCPI-1) in broccoli (Brassica oleracea var. italic) during the regulation of protease activity along postharvest senescence.…”

Section: Plant Proteases and Protease Inhibitorsmentioning

confidence: 99%

Roles of C1A peptidases during barley leaf senescence mediated by abiotic stresses

Arroyo¹

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Overexpression of the protease inhibitor BoCPI-1 in broccoli delays chlorophyll loss after harvest and causes down-regulation of cysteine protease gene expression

Cited by 20 publications

References 56 publications

A Phytocystatin Gene from Malus prunifolia (Willd.) Borkh., MpCYS5, Confers Salt Stress Tolerance and Functions in Endoplasmic Reticulum Stress Response in Arabidopsis

A Phytocystatin Gene from Malus prunifolia (Willd.) Borkh., MpCYS5, Confers Salt Stress Tolerance and Functions in Endoplasmic Reticulum Stress Response in Arabidopsis

The wound response in fresh-cut lettuce involves programmed cell death events

Roles of C1A peptidases during barley leaf senescence mediated by abiotic stresses

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