Overexpression of the GmNAC2 Gene, an NAC Transcription Factor, Reduces Abiotic Stress Tolerance in Tobacco

Jin, Hangxia; Huang, Fang; Cheng, Hao; Song, Haina; Yu, Deyue

doi:10.1007/s11105-012-0514-7

Cited by 56 publications

(29 citation statements)

References 42 publications

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“…In contrast, a few NACs have been shown to suppress drought responses, including GmNAC2 (Jin et al, 2013), OMTN2/3/4/6 (Fang et al, 2014), and NAC016 (Sakuraba et al, 2015). Overexpression of these NACs confers sensitivity to drought stress, whereas their mutation enhances drought tolerance.…”

Section: Discussionmentioning

confidence: 99%

A NAC Transcription Factor Represses Putrescine Biosynthesis and Affects Drought Tolerance

Sun

et al. 2016

Plant Physiology

103

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Arginine decarboxylase (ADC)-mediated putrescine biosynthesis plays an important role in plant stress responses, but the transcriptional regulation of ADC in response to abiotic stress is not well understood. We isolated a NAM, ATAF1/2, and CUC (NAC) domain-containing transcription factor, PtrNAC72, from trifoliate orange (Poncirus trifoliata) by yeast one-hybrid screening. PtrNAC72, localized to the nucleus, binds specifically to the promoter of PtADC and acts as a transcriptional repressor. PtrNAC72 expression was induced by cold, drought, and abscisic acid. ADC messenger RNA abundance and putrescine levels were decreased in transgenic tobacco (Nicotiana nudicaulis) plants overexpressing PtrNAC72 but increased, compared with the wild type, in an Arabidopsis (Arabidopsis thaliana) transfer DNA insertion mutant, nac72 While transgenic tobacco lines overexpressing PtrNAC72 were more sensitive to drought, plants of the Arabidopsis nac72 mutant exhibited enhanced drought tolerance, consistent with the accumulation of reactive oxygen species in the tested genotypes. In addition, exogenous application of putrescine to the overexpression lines restored drought tolerance, while treatment with d-arginine, an ADC inhibitor, compromised the drought tolerance of nac72 Taken together, these results demonstrate that PtrNAC72 is a repressor of putrescine biosynthesis and may negatively regulate the drought stress response, at least in part, via the modulation of putrescine-associated reactive oxygen species homeostasis.

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Section: Discussionmentioning

confidence: 99%

A NAC Transcription Factor Represses Putrescine Biosynthesis and Affects Drought Tolerance

Sun

et al. 2016

Plant Physiology

103

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“…Pons et al (2014) proposed that another TF (TT19) is related to the accumulation of anthocyanin and proanthocyanin, and is involved in cell wall composition and sensitivity to chilling. The genes ppa020620m, ppa025596m, and ppa008301m encoding for NAC TFs are specific to plants and are involved in MD Romeu et al 2014), ripening (Giovannoni et al 1995;Thompson et al 1999;Giovannoni 2007;Shan et al 2012;Zhu et al 2014), biotic and abiotic stress (Fujita et al 2004;He et al 2005;Nakashima et al 2007;Hu et al 2008;Nakashima et al 2012;Jin et al 2013). Furthermore, these TFs are involved in the ABA-dependent response to dehydration, reactive oxygen species detoxification and defense (Fujita et al 2004).…”

Section: Candidate Genes For M Md and Srmentioning

confidence: 99%

Identification of candidate genes associated with mealiness and maturity date in peach [Prunus persica (L.) Batsch] using QTL analysis and deep sequencing

Núñez-Lillo

Cifuentes-Esquivel

Troggio

et al. 2015

Tree Genetics & Genomes

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Artículo de publicación ISIPeach and nectarine quality traits such as flavor, texture, and juiciness are important for consumer acceptance. Maturity date (MD) also plays a role in the fruit-ripening process and is an important factor for marketing fresh fruit. On the other hand, cold storage produces a physiological disorder known as chilling injury where the most important symptom is a lack of juice in the flesh or mealiness (M). In this study, we analyzed an F2 population obtained from a self-pollination of "Venus" nectarine that segregates for MD and M. We built a linkage map with 1,830 SNPs, 7 SSRs and two slow-ripening (SR) morphological markers, spanning 389.2 cM distributed over eight linkage groups (LGs). The SR trait was mapped to LG4 and we compared the whole genome sequences of a SR individual and "Venus" and identified a deletion of 26.6 kb containing ppa008301m (ANAC072) co-localized with the SR trait. Three Quantitative Trait Loci (QTL) for MD were detected; they all co-localize on LG4 between 31.0 and 42.0 cM. Four co-localizing QTLs on LG4 between 33.3 and 40.3 cM were detected for M, explaining 34 % of the phenotypic variation. We identified five and nine candidate genes (CGs) for MD and M from the QTL regions, respectively. Our results suggest that the transcription factors (TFs) ANAC072 and ppa010982m (ERF4) are CGs for both traits. LG4 contains a cluster for genetic factors that possibly regulate MandMD, but functional validation is necessary to unravel the complexity of genetic control responsible for fruit traits.Conicyt-Fondecyt 11121396 Conicyt-FONDEF G13i10005 Corfo-Innova 09PMG-7240 Fondo de Areas Prioritarias Centro de Regulacion del Genoma 15090007 UNAB DI-489-14R CONICYT D-21090737 PFB-1

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“…In the process of plant response to stress, some NAC transcription factors also have a negative regulatory effect on plant response to stress. Glycine max NAC-like gene 2 (GmNAC2) functions as a negative regulator during abiotic stress, and participates in reactive oxygen species (ROS) signaling pathways through modulation of the expression of genes related to ROS scavenging [24].However, the roles of NAC genes in Malus plant stress responses are not well known. Malus baccata is widely used as an apple rootstock ornamental tree all over the world.…”

mentioning

confidence: 99%

Overexpression of a Malus baccata NAC Transcription Factor Gene MbNAC25 Increases Cold and Salinity Tolerance in Arabidopsis

Han

Zhou

et al. 2020

IJMS

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NAC (no apical meristem (NAM), Arabidopsis thaliana transcription activation factor (ATAF1/2) and cup shaped cotyledon (CUC2)) transcription factors play crucial roles in plant development and stress responses. Nevertheless, to date, only a few reports regarding stress-related NAC genes are available in Malus baccata (L.) Borkh. In this study, the transcription factor MbNAC25 in M. baccata was isolated as a member of the plant-specific NAC family that regulates stress responses. Expression of MbNAC25 was induced by abiotic stresses such as drought, cold, high salinity and heat. The ORF of MbNAC25 is 1122 bp, encodes 373 amino acids and subcellular localization showed that MbNAC25 protein was localized in the nucleus. In addition, MbNAC25 was highly expressed in new leaves and stems using real-time PCR. To analyze the function of MbNAC25 in plants, we generated transgenic Arabidopsis plants that overexpressed MbNAC25. Under low-temperature stress (4 • C) and high-salt stress (200 mM NaCl), plants overexpressing MbNAC25 enhanced tolerance against cold and drought salinity conferring a higher survival rate than that of wild-type (WT). Correspondingly, the chlorophyll content, proline content, the activities of antioxidant enzymes superoxide dismutase (SOD), peroxidase (POD) and catalase (CAT) were significantly increased, while malondialdehyde (MDA) content was lower. These results indicated that the overexpression of MbNAC25 in Arabidopsis plants improved the tolerance to cold and salinity stress via enhanced scavenging capability of reactive oxygen species (ROS). The proteins encoded by NAC family genes form homodimers or heterodimers at the N-terminal, consist of about 150 amino acids. The N-terminal regions of NAC proteins share a conserved DNA binding region, which is divided into five subdomains (A-E) [8], whereas the C-terminal region that contains a transcriptional regulatory domain is highly diversified.The regulatory mechanism of NAC TFs consists of two types. The first one is the regulation of transcriptional levels including phosphorylation and ubiquitination miRNAs, which can regulate the expression of TFs at the protein level. The other is that post-transcriptional regulation can be carried out by binding to the target mRNA. NAC transcription factors can regulate target genes by binding to NACRS (NAC recognition sequence) or NACBS (NAC binding sequence), thereby affecting fruit appearance, ripeness, aging, flavor and nutritional quality [9,10]. In addition, NAC transcription factors also bind DNA or other protein kinases to regulate growth and development in plants and various physiological processes, including top meristem [11], secondary wall formation [12], flower organ development [13], bud differentiation [14], embryo development [15], lateral root formation [16,17], plant organ aging and fruit maturity [18].Abiotic stress factors such as drought, high salt, cold and low temperature seriously affect the growth of plants. Under these stresses, a large number of reactive oxygen species will be gener...

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Overexpression of the GmNAC2 Gene, an NAC Transcription Factor, Reduces Abiotic Stress Tolerance in Tobacco

Cited by 56 publications

References 42 publications

A NAC Transcription Factor Represses Putrescine Biosynthesis and Affects Drought Tolerance

A NAC Transcription Factor Represses Putrescine Biosynthesis and Affects Drought Tolerance

Identification of candidate genes associated with mealiness and maturity date in peach [Prunus persica (L.) Batsch] using QTL analysis and deep sequencing

Overexpression of a Malus baccata NAC Transcription Factor Gene MbNAC25 Increases Cold and Salinity Tolerance in Arabidopsis

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