Overexpression of the Gibberellin 2-Oxidase Gene from Camellia lipoensis Induces Dwarfism and Smaller Flowers in Nicotiana tabacum

Xiao, Zheng; Fu, Ruipeng; Liang, Lijian; Fan, Zhengqi; Yin, Hengfu

doi:10.1007/s11105-015-0917-3

Cited by 31 publications

(21 citation statements)

References 32 publications

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“…The positive impact of BAP on shoot length was also indicated by Siwach and Gill [37] in Ficus religiosa. Xiao et al [44] reported that GA 3 had a massive impact on shoot elongation and cell division. Also, many studies proved the positive effect of GA 3 on the elongation of in vitro shoots in the medium [13], [39], [44].…”

Section: Resultsmentioning

confidence: 99%

“…Xiao et al [44] reported that GA 3 had a massive impact on shoot elongation and cell division. Also, many studies proved the positive effect of GA 3 on the elongation of in vitro shoots in the medium [13], [39], [44]. However, some studies figured out that transferring shoots into MS medium in combination with low PGRs or free PGR medium is the successful way for shoot elongation [36].…”

Section: Resultsmentioning

confidence: 99%

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Effect of plant growth regulators on indirect shoot organogenesis of Ficus religiosa through seedling derived petiole segments

Hesami

Daneshvar

Yoosefzadeh-Najafabadi

et al. 2018

Journal of Genetic Engineering and Biotechnology

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Ficus religiosa is known as a long-lived multipurpose forest tree. The tree plays an important role for religious, medicinal, and ornamental purposes. However, the propagation rate of Ficus religiosa is low in natural habitat so the plant tissue culture techniques are an applicable method for multiplication of this valuable medicinal plants. Thus, the aim of this study is to understand the effect of different auxin/cytokinin ratios on indirect shoot organogenesis of this plant. According to our results, the maximum callus induction frequency (100%) was obtained on Murashige and Skoog (MS) medium supplemented with 0.5 mg/l 2,4-dichlorophenoxyacetic acid (2,4-D) plus 0.05 mg/l 6-benzylaminopurine (BAP) from petiole segments. For shoot induction purpose, the yellow-brownish, friable, organogenic calli were inoculated on shoot induction medium. On MS medium supplemented with 1.5 mg/l BAP and 0.15 mg/l Indole-3-butyric acid (IBA), 96.66% of the petiole-derived calli responded with an average number of 3.56 shoots per culture. The highest root formation frequency (96.66%), root number (5.5), and root length (4.83 cm) were achieved on MS medium containing 2.0 mg/l IBA plus 0.1 mg/l Naphthaleneacetic acid (NAA). The rooted shoots were successfully transferred to field condition and the substrate with the mixture of cocopeat and perlite (1:1) had the highest survival rate (96.66%). This is the first report of an effective in vitro organogenesis protocol for F. religiosa by indirect shoot organogenesis through axenic seedling derived petiole explants, which can be efficiently employed for conservation of this important medicinal plant species as well as the utilization of active biomolecules.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Effect of plant growth regulators on indirect shoot organogenesis of Ficus religiosa through seedling derived petiole segments

Hesami

Daneshvar

Yoosefzadeh-Najafabadi

et al. 2018

Journal of Genetic Engineering and Biotechnology

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“…The genes involved in GA metabolism have been isolated in a few plant species, including grape (Wang et al, 2012 ), Arabidopsis thaliana (Phillips et al, 1995 ), tomato (Rebers, 1999 ), Camellia lipoensis (Xiao et al, 2016 ), tomato (Shen et al, 2016 ), and pumpkin (Yamaguchi et al, 1996 ). Although these genes were found to affect the synthesis of GA, their expression patterns were different.…”

Section: Introductionmentioning

confidence: 99%

Genetic Regulation of GA Metabolism during Vernalization, Floral Bud Initiation and Development in Pak Choi (Brassica rapa ssp. chinensis Makino)

et al. 2017

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Pak choi (Brassica rapa ssp. chinensis Makino) is a representative seed vernalization vegetable and premature bolting in spring can cause significant economic loss. Thus, it is critical to elucidate the mechanism of molecular regulation of vernalization and floral bud initiation to prevent premature bolting. Gibberellin (GA) is the key plant hormone involved in regulating plant development. To gain a better understanding of GA metabolism in pak choi, the content of GA in pak choi was measured at different stages of plant development using enzyme-linked immunosorbent assay. The results showed that the GA content increased significantly after low-temperature treatment (4°C) and then decreased rapidly with vegetative growth. During floral bud initiation, the GA content increased rapidly until it peaked upon floral bud differentiation. To elucidate these changes in GA content, the expression of homologous genes encoding enzymes directly involved in GA metabolism were analyzed. The results showed that the changes in the expression of four genes involved in GA synthesis (Bra035120 encoding ent-kaurene synthase, Bra009868 encoding ent-kaurene oxidase, Bra015394 encoding ent-kaurenoic acid oxidase, and Bra013890 encoding GA20-oxidase) were correlated with the changes in GA content. In addition, by comparing the expression of genes involved in GA metabolism at different growth stages, seven differentially expressed genes (Bra005596, Bra009285, Bra022565, Bra008362, Bra033324, Bra010802, and Bra030500) were identified. The differential expression of these genes were directly correlated with changes in GA content, suggesting that these genes were directly related to vernalization, floral bud initiation and development. These results contribute to the understanding of the molecular mechanism of changes in GA content during different developmental phases in pak choi.

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“…reported that the conducive effect of BAP on shoot length was also reached in F. religiosa. Gibberellins exert a positive influence on cell division and shoot elongation (Xiao et al, 2016). Other studies proved that Gibberellin in the medium promotes the elongation of in vitro shoots (Inthima et al, 2017;.…”

Section: Resultsmentioning

confidence: 98%

In Vitro Adventitious Shoot Regeneration through Direct and Indirect Organogenesis from Seedling-derived Hypocotyl Segments of Ficus religiosa L.: An Important Medicinal Plant

Hesami

Daneshvar

2018

horts

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Ficus religiosa is an important industrial, medicinal, and ornamental plant, so in vitro regeneration is of high paramount in this valuable germplasm. Two efficient protocols were developed for indirect and direct shoot organogenesis through hypocotyl explants. In the first experiment, different concentrations of 2,4-dichlorophenoxyacetic acid (2,4-D) and indole butyric acid (IBA) (0.5, 1.0, and 1.5 mg·L−1) in combination with 6-benzyl amino purine (BAP) (ratio 10:1, respectively) were used for callus formation. Two types of callus were obtained from different concentrations of plant growth regulators (PGRs). Also, 2,4-D produced yellow-brownish and friable callus (Type I), whereas the green and compact callus (Type II) was achieved in IBA. The highest callus fresh weight (2.43 g) was observed in Murashige and Skoog (MS) medium containing 0.5 mg·L−1 2,4-D plus 0.05 mg·L−1 BAP. In the later experiments, various concentrations of thidiazuron (TDZ), 6-furfuryl amino purine (KN), and BAP (0.25, 0.5, 1.0, and 1.5 mg·L−1) in combination with IBA (ratio 10:1, respectively) were applied for shoot regeneration (direct and indirect organogenesis). In shoot regeneration from callus, the highest regeneration frequency (86.66%) and shoot number per callus (4.13) were achieved in MS medium supplemented with 1.5 mg·L−1 BAP plus 0.15 mg·L−1 IBA from type I calli. However, no regeneration was observed in type II calli. In direct shoot regeneration, the highest regeneration frequency (96.66%) and shoot number (6.26) were obtained in the medium mentioned previously. In root induction experiment, different concentrations of naphthalene acetic acid (NAA) and IBA alone or in combination were applied, and MS medium containing 2.0 mg·L−1 IBA along with 0.1 mg·L−1 NAA was the best hormonal balance for root induction. The rooted plantlets’ survival rate was more than 95% in the acclimatization stage. These results demonstrated that the direct regeneration method provides more shoot regeneration frequency and take a less time for shoot organogenesis than the indirect regeneration method. Based on our knowledge, this study is the first report of direct and indirect shoot organogenesis of F. religiosa via hypocotyl from in vitro–grown seedling.

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Overexpression of the Gibberellin 2-Oxidase Gene from Camellia lipoensis Induces Dwarfism and Smaller Flowers in Nicotiana tabacum

Cited by 31 publications

References 32 publications

Effect of plant growth regulators on indirect shoot organogenesis of Ficus religiosa through seedling derived petiole segments

Effect of plant growth regulators on indirect shoot organogenesis of Ficus religiosa through seedling derived petiole segments

Genetic Regulation of GA Metabolism during Vernalization, Floral Bud Initiation and Development in Pak Choi (Brassica rapa ssp. chinensis Makino)

In Vitro Adventitious Shoot Regeneration through Direct and Indirect Organogenesis from Seedling-derived Hypocotyl Segments of Ficus religiosa L.: An Important Medicinal Plant

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