Overexpression of the ethylene response factor SlERF1 gene enhances resistance of tomato fruit to Rhizopus nigricans

Pan, Xiaoqi; Fu, Daqi; Zhu, Beiwei; Lü, Chao; Luo, Yunbo

doi:10.1016/j.postharvbio.2012.07.008

Cited by 31 publications

(24 citation statements)

References 58 publications

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“…Up to date, the induced biosynthesis of plant hormone ethylene incorporated with the response to environmental stresses and regulation of fruit ripening has been comprehensively investigated [36], [37]. From our former research work, the invasion of R. nigricans to tomato fruit could induce the endogenous ethylene production [1]. And almost meanwhile, the ethylene-dependent resistance pathways are launched.…”

Section: Resultsmentioning

confidence: 99%

“…Additionally, the E8 could lead to a corresponding reduction in ethylene synthesis in reverse [38]. This may explain why the ethylene production at 48 HPI displayed a downtrend when compared with that at 36 HPI [1].…”

Section: Resultsmentioning

confidence: 99%

“…After removing mycelial debris by filtration, spores were quantified with hemacytometer and diluted to 800 conidia µl −1 for inoculation. Fruit were inoculated with 5 µL of inoculum at each site according to the method described by Pan et al (2013) [1]. The fruit in negative control group was wounded and treated with 5 µL of sterile water at each site.…”

Section: Methodsmentioning

confidence: 99%

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Unraveling the Protein Network of Tomato Fruit in Response to Necrotrophic Phytopathogenic Rhizopus nigricans

et al. 2013

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Plants are endowed with a sophisticated defense mechanism that gives signals to plant cells about the immediate danger from surroundings and protects them from pathogen invasion. In the search for the particular proteins involved in fruit defense responses, we report here a comparative analysis of tomato fruit (Solanum lycopersicum cv. Ailsa Craig) infected by Rhizopus nigricans Ehrenb, which is a significant contributor to postharvest rot disease in fresh tomato fruits. In total, four hundred forty-five tomato proteins were detected in common between the non-infected group and infected tomato fruit of mature green. Forty-nine differentially expressed spots in 2-D gels were identified, and were sorted into fifteen functional groups. Most of these proteins participate directly in the stress response process, while others were found to be involved in several equally important biological processes: protein metabolic process, carbohydrate metabolic process, ethylene biosynthesis, and cell death and so on. These responses occur in different cellular components, both intra- and extracellular spaces. The differentially expressed proteins were integrated into several pathways to show the regulation style existing in tomato fruit host. The composition of the collected proteins populations and the putative functions of the identified proteins argue for their roles in pathogen-plant interactions. Collectively results provide evidence that several regulatory pathways contribute to the resistance of tomato fruit to pathogen.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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Unraveling the Protein Network of Tomato Fruit in Response to Necrotrophic Phytopathogenic Rhizopus nigricans

et al. 2013

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“…For example, the protein kinase LeCTR4 is up-regulated in infected RR fruit, and LeERF1 expression increased in infected MG fruit but is reduced in infected RR fruit. Even though LeERF1 has been reported to induce fruit ripening and softening (Li et al, 2007), its over-expression also is associated with resistance of RR tomato fruit to the necrotroph, Rhizopus nigricans (Pan et al, 2013). In addition, ERF1 serves as an intersection point between ET and JA response pathways triggering plant defenses, particularly against necrotrophs (Lorenzo and Solano, 2005; Pieterse et al, 2012).…”

Section: Resultsmentioning

confidence: 99%

Tomato transcriptome and mutant analyses suggest a role for plant stress hormones in the interaction between fruit and Botrytis cinerea

Blanco‐Ulate¹,

Vincenti²,

Powell³

et al. 2013

Front. Plant Sci.

127

143

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Fruit–pathogen interactions are a valuable biological system to study the role of plant development in the transition from resistance to susceptibility. In general, unripe fruit are resistant to pathogen infection but become increasingly more susceptible as they ripen. During ripening, fruit undergo significant physiological and biochemical changes that are coordinated by complex regulatory and hormonal signaling networks. The interplay between multiple plant stress hormones in the interaction between plant vegetative tissues and microbial pathogens has been documented extensively, but the relevance of these hormones during infections of fruit is unclear. In this work, we analyzed a transcriptome study of tomato fruit infected with Botrytis cinerea in order to profile the expression of genes for the biosynthesis, modification and signal transduction of ethylene (ET), salicylic acid (SA), jasmonic acid (JA), and abscisic acid (ABA), hormones that may be not only involved in ripening, but also in fruit interactions with pathogens. The changes in relative expression of key genes during infection and assays of susceptibility of fruit with impaired synthesis or perception of these hormones were used to formulate hypotheses regarding the involvement of these regulators in the outcome of the tomato fruit–B. cinerea interaction.

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“…Ethylene biosynthesis and signal transduction pathway are complex processes involving the coordinated regulation of gene expression. ERF1 belongs to a large family of plant‐specific transcription factors and has been regarded as acting at the last step of the ethylene signaling pathway (Ohme‐Takagi and Shinshi , Guo and Ecker , Pan et al ). Biochemical and genetic studies have identified the ethylene‐responsive gene ERF1 as an immediate target for EIN3.…”

Section: Discussionmentioning

confidence: 99%

Integrative analysis of circRNAs acting as ceRNAs involved in ethylene pathway in tomato

Wang

Gao

et al. 2017

Physiologia Plantarum

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Circular RNAs (circRNAs) are a large class of non-coding endogenous RNAs that could act as competing endogenous RNAs (ceRNAs) to terminate the mRNA targets' suppression of miRNAs. To elucidate the intricate regulatory roles of circRNAs in the ethylene pathway in tomato fruit, deep sequencing and bioinformatics methods were performed. After strict screening, a total of 318 circRNAs were identified. Among these circRNAs, 282 were significantly differentially expressed among wild-type and sense-/antisense-LeERF1 transgenic tomato fruits. Besides, 1254 target genes were identified and a large amount of them were found to be involved in ethylene pathway. In addition, a sophisticated regulatory model consisting of circRNAs, target genes and ethylene was set up. Importantly, 61 circRNAs were found to be potential ceRNAs to combine with miRNAs and some of the miRNAs had been revealed to participate in the ethylene signaling pathway. This research further raised the possibility that the ethylene pathway in tomato fruit may be under the regulation of various circRNAs and provided a new perspective of the roles of circRNAs.

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Overexpression of the ethylene response factor SlERF1 gene enhances resistance of tomato fruit to Rhizopus nigricans

Cited by 31 publications

References 58 publications

Unraveling the Protein Network of Tomato Fruit in Response to Necrotrophic Phytopathogenic Rhizopus nigricans

Unraveling the Protein Network of Tomato Fruit in Response to Necrotrophic Phytopathogenic Rhizopus nigricans

Tomato transcriptome and mutant analyses suggest a role for plant stress hormones in the interaction between fruit and Botrytis cinerea

Integrative analysis of circRNAs acting as ceRNAs involved in ethylene pathway in tomato

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