Overexpression of the CaHB12 transcription factor in cotton (Gossypium hirsutum) improves drought tolerance

Basso, Marcos Fernando; Costa, Julia Almeida; Ribeiro, Thuanne Pires; Arraes, Fabrício Barbosa Monteiro; Lourenço-Tessutti, Isabela Tristan; Macedo, Amanda Ferreira; Neves, Maysa Rosa das; Nardeli, Sarah Muniz; Arge, Luis Willian Pacheco; Pérez, Carlos Eduardo Aucique; Rodrigues-Silva, Paolo Lucas; Macedo, Leonardo Lima Pepino; Lisei-de-Sá, Maria Eugênia; Amorim, Regina Maria Santos de; Pinto, Eduardo Romano de Campos; Silva, Maria Cristina Mattar; Morgante, Carolina Vianna; Floh, Eny Iochevet Segal; Alves‐Ferreira, Márcio; Grossi-de-Sá, Maria Fátima

doi:10.1016/j.plaphy.2021.05.009

Cited by 12 publications

(5 citation statements)

References 100 publications

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“…The expression of the salt tolerance gene MsAnn2 in alfalfa was enhanced under salt stress [54]. To better understand the resistance of GhANN to abiotic stress, we examined the expression of marker genes associated with abiotic stress, including GhCBL3, GhDREB2A, GhDREB2C, GhRD20-2, GhSOS1, and GhNHX1 [55][56][57][58][59][60][61][62][63][64][65][66][67][68], in TRV:00 and TRV:GhANN4 plants. Our results showed that the expression levels of GhCBL3, GhDREB2A, GhDREB2C, GhPP2C, and GhRD20-2 in GhANN4-silenced plants were significantly downregulated under drought stress, and the expression levels of GhCIRK6, GhNHX1, GhRD20-1, GhSOS1, GhSOS2, and GhSNRK2.6 were also significantly downregulated under salt stress, suggesting that silencing GhANN4 may weaken the ROS clearance ability of G. hirsutum by reducing the expression of these genes, thus reducing drought and salt tolerance.…”

Section: Discussionmentioning

confidence: 99%

Genome-Wide Identification of the GhANN Gene Family and Functional Validation of GhANN11 and GhANN4 under Abiotic Stress

Luo,

Li,

et al. 2024

IJMS

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Annexins (ANNs) are a structurally conserved protein family present in almost all plants. In the present study, 27 GhANNs were identified in cotton and were unevenly distributed across 14 chromosomes. Transcriptome data and RT-qPCR results revealed that multiple GhANNs respond to at least two abiotic stresses. Similarly, the expression levels of GhANN4 and GhANN11 were significantly upregulated under heat, cold, and drought stress. Using virus-induced gene silencing (VIGS), functional characterization of GhANN4 and GhANN11 revealed that, compared with those of the controls, the leaf wilting of GhANN4-silenced plants was more obvious, and the activities of catalase (CAT), peroxidase (POD), and superoxide dismutase (SOD) were lower under NaCl and PEG stress. Moreover, the expression of stress marker genes (GhCBL3, GhDREB2A, GhDREB2C, GhPP2C, GhRD20-2, GhCIPK6, GhNHX1, GhRD20-1, GhSOS1, GhSOS2 and GhSnRK2.6) was significantly downregulated in GhANN4-silenced plants after stress. Under cold stress, the growth of the GHANN11-silenced plants was significantly weaker than that of the control plants, and the activities of POD, SOD, and CAT were also lower. However, compared with those of the control, the elasticity and orthostatic activity of the GhANN11-silenced plants were greater; the POD, SOD, and CAT activities were higher; and the GhDREB2C, GhHSP, and GhSOS2 expression levels were greater under heat stress. These results suggest that different GhANN family members respond differently to different types of abiotic stress.

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Section: Discussionmentioning

confidence: 99%

Genome-Wide Identification of the GhANN Gene Family and Functional Validation of GhANN11 and GhANN4 under Abiotic Stress

Luo,

Li,

et al. 2024

IJMS

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Section: Discussionmentioning

confidence: 99%

“…In contrast, there are several works in which the over or ectopic expression of these genes conferred potential beneficial traits, such as increased tolerance to abiotic stress factors, in many plant species (Manavella et al ., 2006; Cabello et al ., 2012; Cabello et al ., 2016; Y. Zhao et al ., 2014; Basso et al ., 2021; S. Zhao et al ., 2021; Raineri et al ., 2022). Moreover, several constructs able to overexpress HD-Zip I members and others to knock-down these genes were protected with patents (Arce et al ., 2008; Guo et al ., 2011; Sanz-Molinero et al ., 2016).…”

Section: Discussionmentioning

confidence: 99%

Source to sink partitioning is altered by the expression of the transcription factor AtHB5

Raminger

Miguel

Zapata

et al. 2022

Preprint

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Carbohydrates are transported from source to sink tissues. The efficiency of such transport determines plant growth and development. The process is finely regulated, and transcription factors are crucial in such modulation. AtHB5 is a homeodomain-leucine zipper I transcription factor, repressed during stem secondary growth. However, its function in this developmental event was unknown. Here, we investigated the expression pattern and role of AtHB5. AtHB5 localized in conductive tissues: roots, hypocotyls, stems, pedicels, and central leaf veins. Mutant plants exhibited wider and more lignified stems than controls, whereas overexpressors showed the opposite phenotype. Cross-sections of athb5 mutant stems showed enlarged vascular bundle, xylem, phloem, and petiole areas, whereas AtHB5 overexpressors exhibited callose deposits. Several genes involved in starch biosynthesis and degradation had altered transcript levels in athb5 mutants and AtHB5 overexpressors. Rosette and stem biomasses were enhanced in athb5 mutants, positively impacting seed yield and lipid content. Moreover, these effects were more evident in debranched plants. Finally, the transport to roots significantly slowed down in AtHB5 overexpressors. Altogether, the results indicated that AtHB5 is a negative modulator of sucrose transport from source to sink tissues, and its overexpression diminished plant biomass and seed yield.

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“…Furthermore, overexpression of the drought-responsive AP2/ERF transcription factor gene OsERF71 enhanced drought tolerance in transgenic rice by increasing the lignification level in roots, resulting in root architecture alteration (Lee et al, 2016). Transgenic cotton plants overexpressing the Coffea arabica HB12 gene, CaHB12, and Gossypium hirsutum bZIP transcription factor gene, GhABF2, independently, showed improved drought tolerance by up-regulation of genes in the ABA-dependent signaling pathway (Liang et al, 2016b;Basso et al, 2021). Recently, Gonzaĺez et al (2019) overexpressed the sunflower gene HaHB4 that encodes a protein in the homeodomain-leucine zipper I family in wheat, and they showed that transgenic wheat grown in the field under drought conditions outperformed wild-type wheat.…”

Section: Improved Drought Tolerance In Transgenic Cropsmentioning

confidence: 99%

Genetic manipulation for abiotic stress resistance traits in crops

2022

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Abiotic stresses are major limiting factors that pose severe threats to agricultural production. Conventional breeding has significantly improved crop productivity in the last century, but traditional breeding has reached its maximum capacity due to the multigenic nature of abiotic stresses. Alternatively, biotechnological approaches could provide new opportunities for producing crops that can adapt to the fast-changing environment and still produce high yields under severe environmental stress conditions. Many stress-related genes have been identified and manipulated to generate stress-tolerant plants in the past decades, which could lead to further increase in food production in most countries of the world. This review focuses on the recent progress in using transgenic technology and gene editing technology to improve abiotic stress tolerance in plants, and highlights the potential of using genetic engineering to secure food and fiber supply in a world with an increasing population yet decreasing land and water availability for food production and fast-changing climate that will be largely hostile to agriculture.

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Overexpression of the CaHB12 transcription factor in cotton (Gossypium hirsutum) improves drought tolerance

Cited by 12 publications

References 100 publications

Genome-Wide Identification of the GhANN Gene Family and Functional Validation of GhANN11 and GhANN4 under Abiotic Stress

Genome-Wide Identification of the GhANN Gene Family and Functional Validation of GhANN11 and GhANN4 under Abiotic Stress

Source to sink partitioning is altered by the expression of the transcription factor AtHB5

Genetic manipulation for abiotic stress resistance traits in crops

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