Overexpression of the Arabidopsis MACPF Protein AtMACP2 Promotes Pathogen Resistance by Activating SA Signaling

Zhang, Xue; Dai, Yangshuo; Wang, Yuxin; Su, Ze-Zhuo; Yu, Lujun; Zhang, Zhen-Fei; Xiao, Shi; Chen, Qinfang

doi:10.3390/ijms23158784

Cited by 8 publications

(14 citation statements)

References 77 publications

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“…Other putative targets with defence‐ and immunity‐related functions include, for example, the soybean homologues of Arabidopsis MEMBRANE ATTACK COMPLEX/PERFORIN‐LIKE 1 (MACP1, Glyma.11G252700), which is involved in salicylic acid accumulation and programmed cell death in response to pathogen infection (Zhang et al., 2022 ); the pleiotropic drug resistance transporters PDR12 (Glyma.13G361900) and PEN3 (Glyma.08G201300), which function redundantly in the secretion of the phytoalexin camalexin (He et al., 2019 ); and RESPIRATORY BURST OXIDASE HOMOLOG D (RBOHD, Glyma.04G203200), which is believed to be the primary source of ROS during PTI and plays an essential role in plant immunity (Kadota et al., 2015 ; O'Brien et al., 2012 ) and plant–nematode interactions (Chopra et al., 2021 ; Siddique et al., 2014 ; Teixeira et al., 2016 ). However, further studies are needed to determined how phosphorylation status of these proteins induces functional changes leading to specific response to SCN infection.…”

Section: Discussionmentioning

confidence: 99%

Soybean MKK2 establishes intricate signalling pathways to regulate soybean response to cyst nematode infection

Hawk,

Piya,

Sultana

et al. 2024

Molecular Plant Pathology

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Mitogen‐activated protein kinase (MPK) cascades play central signalling roles in plant immunity and stress response. The soybean orthologue of MPK kinase2 (GmMKK2) was recently identified as a potential signalling node whose expression is upregulated in the feeding site induced by soybean cyst nematode (SCN, Heterodera glycines). To investigate the role of GmMKK2 in soybean–SCN interactions, we overexpressed a catabolically inactive variant referred to as kinase‐dead variant (KD‐GmMKK2) using transgenic hairy roots. KD‐GmMKK2 overexpression caused significant reduction in soybean susceptibility to SCN, while overexpression of the wild‐type variant (WT‐GmMKK2) exhibited no effect on susceptibility. Transcriptome analysis indicated that KD‐GmMKK2 overexpressing plants are primed for SCN resistance via constitutive activation of defence signalling, particularly those related to chitin, respiratory burst, hydrogen peroxide and salicylic acid. Phosphoproteomic profiling of the WT‐GmMKK2 and KD‐GmMKK2 root samples upon SCN infection resulted in the identification of 391 potential targets of GmMKK2. These targets are involved in a broad range of biological processes, including defence signalling, vesicle fusion, chromatin remodelling and nuclear organization among others. Furthermore, GmMKK2 mediates phosphorylation of numerous transcriptional and translational regulators, pointing to the presence of signalling shortcuts besides the canonical MAPK cascades to initiate downstream signalling that eventually regulates gene expression and translation initiation. Finally, the functional requirement of specific phosphorylation sites for soybean response to SCN infection was validated by overexpressing phospho‐mimic and phospho‐dead variants of two differentially phosphorylated proteins SUN1 and IDD4. Together, our analyses identify GmMKK2 impacts on signalling modules that regulate soybean response to SCN infection.

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Section: Discussionmentioning

confidence: 99%

Soybean MKK2 establishes intricate signalling pathways to regulate soybean response to cyst nematode infection

Hawk,

Piya,

Sultana

et al. 2024

Molecular Plant Pathology

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“…Ma06_g13550 belongs to the membrane attack complex component/perforin (MACPF) domain-containing protein CAD1 (constitutively activated cell death 1) family, a domain operating at the plasma membrane level and involved in resistance through cellular response to stress, programmed cell death, and the innate immune system. In a model organism, Arabidopsis thaliana, At4g24290 and CAD1 from the same family and the closest orthologs to Ma06_g13550 confer resistance to bacterial pathogens [55,56]. The gene is a close ortholog of Zm00001eb241040 (protein C0PFJ5) in maize [57] and Os02g0475300 (protein Q6K741) in rice [58], with speculated similar functions.…”

Section: Discussionmentioning

confidence: 99%

Identification of the Loci Associated with Resistance to Banana Xanthomonas Wilt (Xanthomonas vasicola pv. musacearum) Using DArTSeq Markers and Continuous Mapping

Uwimana,

Nakato,

Kanaabi

et al. 2024

Horticulturae

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Banana Xanthomonas wilt, caused by Xanthomonas vasicola pv. musacearum (Xvm), is a devastating disease that results in total yield loss of affected plants. Resistance to the disease is limited in Musa acuminata, but it has been identified so far in the zebrina subspecies. This study identified markers associated with tolerance to Xvm in Monyet, a tetraploid banana from the zebrina subspecies which was identified to be partially resistant to the bacterium. We used a triploid progeny of 135 F1 hybrids resulting from a cross between Monyet (Xvm partially resistant) and Kokopo (diploid and Xvm susceptible). The F1 hybrids were screened in pots for resistance to Xvm. The population was genotyped using the genotyping-by-sequencing platform of Diversity Array Technology (DArTSeq). The adjusted means of the phenotypic data were combined with the allele frequencies of the genotypic data in continuous mapping. We identified 25 SNPs associated with resistance to Xvm, and these were grouped into five quantitative traits loci (QTL) on chromosomes 2, 3, 6, and 7. For each marker, we identified the favorable allele and the additive effect of replacing the reference allele with the alternative allele. The comparison between weevil borer (Cosmopolites sordidus (Germar)) and Xvm QTL revealed one QTL shared between the two biotic stresses at the distal end of chromosome 6 but with a repulsion linkage. This linkage should be broken down by generating more recombinants in the region. We also identified 18 putative alleles in the vicinity of the SNPs associated with resistance to Xvm. Among the 18 putative genes, two particularly putative genes, namely, Ma06_g13550 and Ma06_g36840, are most likely linked to disease resistance. This study is a basis for marker-assisted selection to improve banana resistance to banana Xanthomonas wilt, especially in East and Central Africa where the disease is still devastating the crop.

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“…Bacterial fermentation products and their synthetic derivatives have antitumor properties and strong binding affinity for spliceosome components. , These drugs alter the gene expression of genes important to disease progression including AS. AS makes major contributions to various disease treatments.…”

Section: Alternative Splicing: Potential New Therapeutics Against Inf...mentioning

confidence: 99%

Alternative Splicing, An Overlooked Defense Frontier of Plants with Respect to Bacterial Infection

Xie,

Zhou,

Jia

et al. 2023

J. Agric. Food Chem.

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Disease represents a major problem in sustainable agricultural development. Plants interact closely with various microorganisms during their development and in response to the prevailing environment. In particular, pathogenic microorganisms can cause plant diseases, affecting the fertility, yield, and longevity of plants. During the long coevolution of plants and their pathogens, plants have evolved both molecular pattern-triggered immunity (PTI) and effector-triggered immunity (ETI) signaling networks in order to regulate host cells in response to pathogen infestation. Additionally, in the postgenomic era, alternative splicing (AS) has become uncovered as one of the major drivers of proteome diversity, and abnormal RNA splicing is closely associated with bacterial infections. Currently, the complexity of host−bacteria interactions is a much studied area of research that has shown steady progress over the past decade. Although the development of high-throughput sequencing technologies and their application in transcriptomes have revolutionized our understanding of AS, many mechanisms related to host−bacteria interactions remain still unclear. To this end, this review summarizes the changes observed in AS during host−bacteria interactions and outlines potential therapeutics for bacterial diseases based on existing studies. In doing so, we hope to provide guidelines for plant disease management in agriculture.

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Overexpression of the Arabidopsis MACPF Protein AtMACP2 Promotes Pathogen Resistance by Activating SA Signaling

Cited by 8 publications

References 77 publications

Soybean MKK2 establishes intricate signalling pathways to regulate soybean response to cyst nematode infection

Soybean MKK2 establishes intricate signalling pathways to regulate soybean response to cyst nematode infection

Identification of the Loci Associated with Resistance to Banana Xanthomonas Wilt (Xanthomonas vasicola pv. musacearum) Using DArTSeq Markers and Continuous Mapping

Alternative Splicing, An Overlooked Defense Frontier of Plants with Respect to Bacterial Infection

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