Overexpression of TaFBA-A10 from Winter Wheat Enhances Freezing Tolerance in Arabidopsis thaliana

Peng, Kankan; Tian, Yu; Cang, Jing; Yu, Jun; Wang, Duojia; He, Fei; Jiao, Huarui; Tan, Yige

doi:10.1007/s00344-021-10304-7

Cited by 14 publications

(21 citation statements)

References 77 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In this study, the expression of Dn1 tae-miR399 at -10 was lower than that at 5, but it was higher than that at 0, which was similar to the previous results, indicating that the expression of miR399 was started at -10. At the extremely low temperature of -25, the expression of transcription factors, such asTaMYB1 , TabZIP1 and TaWABI5 , tends to decrease slightly, while the expression of genes encoding key enzymes in metabolism pathway still rises sharply, such as TaCSD1 ,TaG6PDH and Ta6PGDH (Lu, Guo, et al, 2020;Lv et al, 2018;Tian et al, 2021). In this study, we found that at -25, the expression of Dn1 tae-miR399 increased significantly, while the target gene TaUBC24 decreased significantly.…”

Section: Discussionmentioning

confidence: 55%

“…When the temperature drops to -10, the physiological and biochemical level and molecular level of winter wheat begins to change significantly (Lu, Guo, Xu, & Cang, 2020;Lv et al, 2018). Previous studies have shown that most cold resistance genes begins to increase significantly at -10, such as TaWABI5 ,TaCSD1 and TaFBA-A10 (Lu, Guo, et al, 2020;Lv et al, 2018;K. K. Peng et al, 2021).…”

Section: Discussionmentioning

confidence: 99%

“…Winter wheat (Triticum aestivum ) cultivar Dongnongdongmai1 (Dn1) was obtained from the Wheat Breeding Institue of Northeast Agricultural University, Harbin, China. The field trial referred to previous study (Tian et al, 2021),was carried out in autumn, September 8th, 2018, at Northeast Agricultural University Experimental Site (45°7'N, 126°6'E) in Harbin, China. Then the tillering nodes were collected at 5 (October 15th, 2018), 0 (November 2nd, 2018), -10 (November 23rd, 2018) and -25 (January 14th, 2019) (average of minimum temperature for ten consecutive days) and store at -80degC.…”

Section: Plants Materials and Growth Conditionsmentioning

confidence: 99%

“…Tillering node is an important organ to winter wheat overwintering (K. K. Peng et al, 2021). To investigate the changes in tae-miR399 andTaUBC24 expression levels in fields under naturally decreasing temperature, the qRT-PCR was performed in Dn1 tillering nodes.…”

Section: Expression Analysis Of Tae-mir399 and Taubc24 At Freezing Stress In Dn1mentioning

confidence: 99%

“…To avoid winterkill it is very important to obtain the wheat freezing resistance gene (Babben et al, 2018). Dongnongdongmai1 (Dn1) is the first winter wheat cultivar that can overwinter safely in alpine region of Heilongjiang province (The minimum temperature in this area can reach -30) and the rate of returning green is greater than 85% (K. K. Peng et al, 2021;Tian et al, 2021). Winter wheat's resistance to cold includes microRNAs (miRNAs)-short, single-stranded, non-coding RNAs that regulate the posttranscriptional gene expression by targeting mRNAs for cleavage or repressing translation (Lu, Xu, et al, 2020).…”

Section: Introductionmentioning

confidence: 99%

See 4 more Smart Citations

miR399-UBC24 module enhances freezing tolerance through regulating CBF signaling pathway and starch degradation

Peng

Tian

et al. 2021

Preprint

Self Cite

View full text Add to dashboard Cite

Although the regulation in Pi homeostasis of miR399 have been studied in various plants, its molecular mechanisms in response to freezing stress are still elusive. In this work, we found that the expression of tae-miR399 and its target gene TaUBC24 in tillering nodes of strong cold resistance winter wheat cultivar Dongnongdongmai1 (Dn1) was significantly altered subjected to severe winter. tae-miR399 and its target gene TaUBC24 were also responsive to short-term freezing stress in tillering nodes of Dn1 seedlings. TaUBC24 physically interacted with TaICE1. Ehanced freezing tolerance was observed in overexpressing tae-miR399 Arabidopsis lines. Under freezing stress, overexpressing tae-miR399 decreased the expression of AtUBC24 to increase the expression of genes in CBF signaling pathway, Pi translocation pathway and starch metablism, including AtCBFs, AtCOR47, AtCOR413IM, AtPHT1;4, AtAPLs and AtBAMs, inhibit the degradation of AtICE1 and AtPHO1, and promote the activities of SOD, POD and CAT. These findings indicated that the increased freezing tolerance was dependent upon elevating CBF signaling pathway, phosphorus utilization efficiency, starch degradation, accumulation of soluble sugar and ability of ROS scavenge. These results will aid our understanding of molecular mechanism of how miR399-UBC24 module plays a cardinal role in regulating plant freezing stress tolerance through mediating the downstream pathways.

show abstract

Section: Discussionmentioning

confidence: 55%

Section: Discussionmentioning

confidence: 99%

Section: Plants Materials and Growth Conditionsmentioning

confidence: 99%

Section: Expression Analysis Of Tae-mir399 and Taubc24 At Freezing Stress In Dn1mentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

miR399-UBC24 module enhances freezing tolerance through regulating CBF signaling pathway and starch degradation

Peng

Tian

et al. 2021

Preprint

Self Cite

View full text Add to dashboard Cite

show abstract

Daylight ultraviolet B radiation ruptured the cell membrane, promoted nucleotide metabolism and inhibited energy metabolism in the plasma of Pacific oyster

Song

Xie

Kong

et al. 2023

Science of The Total Environment

View full text Add to dashboard Cite

tae-miR399-UBC24 Module Enhances Freezing Tolerance in Winter Wheat via a CBF Signaling Pathway

Peng

Tian

Sun

et al. 2021

J. Agric. Food Chem.

Self Cite

View full text Add to dashboard Cite

Although the regulation of Pi homeostasis by miR399 has been studied in various plant species, its underlying molecular mechanism in response to freezing stress is still poorly understood. In this work, we found that the expression of tae-miR399 and its target gene TaUBC24 in the tillering nodes of the strong cold-resistant winter wheat cultivar Dongnongdongmai1 (Dn1) was not only significantly altered after severe winters but also responsive to short-term freezing stress. TaUBC24 physically interacted with TaICE1. Enhanced freezing tolerance was observed for tae-miR399-overexpressing Arabidopsis lines. Under freezing stress, overexpression of tae-miR399 ultimately decreased the expression of AtUBC24, inhibiting the degradation of AtICE1, which increased the expression of genes involved in the CBF signaling pathway and starch metabolism and promoted the activities of antioxidant enzymes. These results will improve our understanding of the molecular mechanism through which the miR399-UBC24 module plays a cardinal role in regulating plant freezing stress tolerance through mediation of downstream pathways.

show abstract

Overexpression of TaFBA-A10 from Winter Wheat Enhances Freezing Tolerance in Arabidopsis thaliana

Cited by 14 publications

References 77 publications

miR399-UBC24 module enhances freezing tolerance through regulating CBF signaling pathway and starch degradation

miR399-UBC24 module enhances freezing tolerance through regulating CBF signaling pathway and starch degradation

Daylight ultraviolet B radiation ruptured the cell membrane, promoted nucleotide metabolism and inhibited energy metabolism in the plasma of Pacific oyster

tae-miR399-UBC24 Module Enhances Freezing Tolerance in Winter Wheat via a CBF Signaling Pathway

Contact Info

Product

Resources

About