Overexpression of Suppressors of Cytokine Signaling 1 Promotes the Neuronal Differentiation of C17.2 Neural Stem Cells

Cui, Meng; Dai, Bin; Xin, Jing‐yi; He, Jinquan; Feng, Shiqing

doi:10.1159/000358632

Cited by 11 publications

(6 citation statements)

References 23 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The migration distance of neural cells from the edge of the neurospheres was measured by Image-J software. Immunostaining was performed to visualize Nestin (NSCs marker) 23 and microtubule-associated protein-2 (MAP2, neuron marker) 24 as described below.…”

Section: Methodsmentioning

confidence: 99%

“…Single cells were seeded at 2 × 10 5 cells/well onto a six-well PDL-coated plate and then treated with different concentrations of OA (10, 20, and 40 μM). After incubation for 7 days, NSC differentiation was detected by measuring the levels of differentiation markers, including Nestin, MAP2, and glial fibrillary acidic protein (GFAP, astrocytes marker) 24 , by western blotting and immunofluorescence staining.…”

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Oleanolic acid enhances neural stem cell migration, proliferation, and differentiation in vitro by inhibiting GSK3β activity

et al. 2018

View full text Add to dashboard Cite

Oleanolic acid (OA), one of the bioactive ingredients in ginseng, has been reported to have neuroprotective activities. However, the effects and its mechanism on neural stem cell (NSC) induction are not entirely clear. In the present study, we investigated the effects of OA on promoting the migration, proliferation, and differentiation of neural stem cells (NSCs). Migration and proliferation were investigated by using neural-specific markers, neurosphere assay, and Cell Counting Kit-8, respectively. We found OA remarkably promoted neural migration and proliferation of NSCs in a time- and dose-dependent manner. Differentiation was analyzed by western blotting and immunofluorescence staining, which found MAP2 expression was remarkably increased, whereas Nestin was dramatically decreased. In addition, OA increased phosphorylation of GSK3β at Ser9 and expression of active forms of β-catenin. Furthermore, NSCs with constitutively active GSK3β (S9A) significantly suppressed the OA-induced proliferation and neural differentiation. These results showed that OA could stimulate NSC proliferation and neural differentiation in vitro via suppressing the activity of GSK3β. Our findings may have significant implications for the treatment of neurodegenerative diseases.

show abstract

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Oleanolic acid enhances neural stem cell migration, proliferation, and differentiation in vitro by inhibiting GSK3β activity

et al. 2018

View full text Add to dashboard Cite

show abstract

“…During such co-regenerative process, it has also been confirmed to participate in the generation of the nerve system. In 2015, a specific study [ 108 ] on neuron stem cells confirmed that the differentiation of neurons may be regulated by such gene.…”

Section: Discussionmentioning

confidence: 99%

Network-Based Method for Identifying Co-Regeneration Genes in Bone, Dentin, Nerve and Vessel Tissues

Lei

Pan

Zhang

et al. 2017

Genes

View full text Add to dashboard Cite

Bone and dental diseases are serious public health problems. Most current clinical treatments for these diseases can produce side effects. Regeneration is a promising therapy for bone and dental diseases, yielding natural tissue recovery with few side effects. Because soft tissues inside the bone and dentin are densely populated with nerves and vessels, the study of bone and dentin regeneration should also consider the co-regeneration of nerves and vessels. In this study, a network-based method to identify co-regeneration genes for bone, dentin, nerve and vessel was constructed based on an extensive network of protein–protein interactions. Three procedures were applied in the network-based method. The first procedure, searching, sought the shortest paths connecting regeneration genes of one tissue type with regeneration genes of other tissues, thereby extracting possible co-regeneration genes. The second procedure, testing, employed a permutation test to evaluate whether possible genes were false discoveries; these genes were excluded by the testing procedure. The last procedure, screening, employed two rules, the betweenness ratio rule and interaction score rule, to select the most essential genes. A total of seventeen genes were inferred by the method, which were deemed to contribute to co-regeneration of at least two tissues. All these seventeen genes were extensively discussed to validate the utility of the method.

show abstract

“…SOCSs also play important roles in neuronal differentiation and regeneration, neurogenesis, and nervous system development. SOCS1 regulates IFN-γ-mediated survival of sensory neurons [ 100 ] and NSC proliferation and differentiation into neurons [ 101 , 102 ].…”

Section: Characteristics Of Socs Proteins and Their Roles In Neuronal...mentioning

confidence: 99%

Role of SOCS and VHL Proteins in Neuronal Differentiation and Development

Kanno

Matsumoto

Yoshizumi

et al. 2023

IJMS

View full text Add to dashboard Cite

The basic helix–loop–helix factors play a central role in neuronal differentiation and nervous system development, which involve the Notch and signal transducer and activator of transcription (STAT)/small mother against decapentaplegic signaling pathways. Neural stem cells differentiate into three nervous system lineages, and the suppressor of cytokine signaling (SOCS) and von Hippel-Lindau (VHL) proteins are involved in this neuronal differentiation. The SOCS and VHL proteins both contain homologous structures comprising the BC-box motif. SOCSs recruit Elongin C, Elongin B, Cullin5(Cul5), and Rbx2, whereas VHL recruits Elongin C, Elongin B, Cul2, and Rbx1. SOCSs form SBC-Cul5/E3 complexes, and VHL forms a VBC-Cul2/E3 complex. These complexes degrade the target protein and suppress its downstream transduction pathway by acting as E3 ligases via the ubiquitin–proteasome system. The Janus kinase (JAK) is the main target protein of the E3 ligase SBC-Cul5, whereas hypoxia-inducible factor is the primary target protein of the E3 ligase VBC-Cul2; nonetheless, VBC-Cul2 also targets the JAK. SOCSs not only act on the ubiquitin–proteasome system but also act directly on JAKs to suppress the Janus kinase–signal transduction and activator of transcription (JAK-STAT) pathway. Both SOCS and VHL are expressed in the nervous system, predominantly in brain neurons in the embryonic stage. Both SOCS and VHL induce neuronal differentiation. SOCS is involved in differentiation into neurons, whereas VHL is involved in differentiation into neurons and oligodendrocytes; both proteins promote neurite outgrowth. It has also been suggested that the inactivation of these proteins may lead to the development of nervous system malignancies and that these proteins may function as tumor suppressors. The mechanism of action of SOCS and VHL involved in neuronal differentiation and nervous system development is thought to be mediated through the inhibition of downstream signaling pathways, JAK-STAT, and hypoxia-inducible factor–vascular endothelial growth factor pathways. In addition, because SOCS and VHL promote nerve regeneration, they are expected to be applied in neuronal regenerative medicine for traumatic brain injury and stroke.

show abstract

Overexpression of Suppressors of Cytokine Signaling 1 Promotes the Neuronal Differentiation of C17.2 Neural Stem Cells

Cited by 11 publications

References 23 publications

Oleanolic acid enhances neural stem cell migration, proliferation, and differentiation in vitro by inhibiting GSK3β activity

Oleanolic acid enhances neural stem cell migration, proliferation, and differentiation in vitro by inhibiting GSK3β activity

Network-Based Method for Identifying Co-Regeneration Genes in Bone, Dentin, Nerve and Vessel Tissues

Role of SOCS and VHL Proteins in Neuronal Differentiation and Development

Contact Info

Product

Resources

About