Overexpression of StNF-YB3.1 reduces photosynthetic capacity and tuber production, and promotes ABA-mediated stomatal closure in potato ( Solanum tuberosum L.)

Xuanyuan, Guochao; Lu, Congming; Zhang, Ruofang; Jiang, Jiming

doi:10.1016/j.plantsci.2017.04.015

Cited by 21 publications

(20 citation statements)

References 74 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…However, it was found that high-yielding wheat genotypes showed a significant delay of about 5 min in decrease of SC compared to the low-yielding varieties. These results are in accordance with those described above for potato, with overexpression of StNF-YB3.1 causing stomatal closure which also was associated with a significant reduction in tuber yield ( Xuanyuan et al, 2017 ). In bread wheat in water-limited conditions, significant positive correlations between stomatal characteristics (width, length and area) and grain yield were reported ( Arminian et al, 2008 ).…”

Section: Discussionsupporting

confidence: 92%

“…Measurement of stomatal conductance, SC, can indicate how fast plants can react to the stress, and there are several reports concerning the regulation of SC and stomatal closure by TFs. Overexpression of TaNF-YB3;1 in tobacco and StNF-YB3.1 in potato were reported to cause stomatal closure, enhancing the expression of several ABA-related genes ( Xuanyuan et al, 2017 ; Yang et al, 2017 ), while overexpression of StNF-YA7 , encoding a different subunit (NFY-A), reduced water loss and improved tolerance to slow drought in transgenic potato compared to controls ( Na et al, 2017 ). When intact non-stressed plants were treated with ABA in the present studies, they reacted sooner, due to the presence of an intact root system, with SC declining within 5–10 min while detached leaves required 5–15 min ( Figures 4C,D ).…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Genes Encoding Transcription Factors TaDREB5 and TaNFYC-A7 Are Differentially Expressed in Leaves of Bread Wheat in Response to Drought, Dehydration and ABA

et al. 2018

View full text Add to dashboard Cite

Two groups of six spring bread wheat varieties with either high or low grain yield under the dry conditions of Central and Northern Kazakhstan were selected for analysis. Experiments were set up with the selected wheat varieties in controlled environments as follows: (1) slowly progressing drought imposed on plants in soil, (2) rapid dehydration of whole plants grown in hydroponics, (3) dehydration of detached leaves, and (4) ABA treatment of whole plants grown in hydroponics. Representatives of two different families of transcription factors (TFs), TaDREB5 and TaNFYC-A7, were found to be linked to yield-under-drought using polymorphic Amplifluor-like SNP marker assays. qRT-PCR revealed differing patterns of expression of these genes in the leaves of plants subjected to the above treatments. Under drought, TaDREB5 was significantly up-regulated in leaves of all high-yielding varieties tested and down-regulated in all low-yielding varieties, and the level of expression was independent of treatment type. In contrast, TaNFYC-A7 expression levels showed different responses in the high- and low-yield groups of wheat varieties. TaNFYC-A7 expression under dehydration (treatments 2 and 3) was higher than under drought (treatment 1) in all high-yielding varieties tested, while in all low-yielding varieties the opposite pattern was observed: the expression levels of this gene under drought were higher than under dehydration. Rapid dehydration of detached leaves and intact wheat plants grown in hydroponics produced similar changes in gene expression. ABA treatment of whole plants caused rapid stomatal closure and a rise in the transcript level of both genes during the first 30 min, which decreased 6 h after treatment. At this time-point, expression of TaNFYC-A7 was again significantly up-regulated compared to untreated controls, while TaDREB5 returned to its initial level of expression. These findings reveal significant differences in the transcriptional regulation of two drought-responsive and ABA-dependent TFs under slowly developing drought and rapid dehydration of wheat plants. The results obtained suggest that correlation between grain yield in dry conditions and TaNFYC-A7 expression levels in the examined wheat varieties is dependent on the length of drought development and/or strength of drought; while in the case of TaDREB5, no such dependence is observed.

show abstract

Section: Discussionsupporting

confidence: 92%

Section: Discussionmentioning

confidence: 99%

Genes Encoding Transcription Factors TaDREB5 and TaNFYC-A7 Are Differentially Expressed in Leaves of Bread Wheat in Response to Drought, Dehydration and ABA

et al. 2018

View full text Add to dashboard Cite

show abstract

“…to control stomata closure in response to water-deficit stress (Xuanyuan et al, 2017). After ABA processing the OST1 expression was lower in the T-DNA mutant ckb3 than in the Col-0 plants.…”

Section: Discussionmentioning

confidence: 98%

Arabidopsis CK2 family gene CKB3 involved in abscisic acid signaling

et al. 2021

View full text Add to dashboard Cite

CKB3 is a regulatory (beta) subunit of CK2. In this study Arabidopsis thaliana homozygous T-DNA mutant ckb3 was studied to understand the role of CKB3 in abscisic acid (ABA) signaling. The results shown: CKB3 was expressed in all organs and the highest expression in the seeds, followed by the root. During seed germination and root growth the ckb3 mutant showed reduced sensitivity to ABA. The ckb3 mutant had more stomatal opening and increased proline accumulation and leaf water loss. The expression levels of number of genes in the ABA regulatory network had changed. This study demonstrates that CKB3 is an ABA signaling-related gene and may play a positive role in ABA signaling.

show abstract

“…2b). It suggesting that PtNF-YB1 may be part of the regulation of flowering pathway, just like AtNF-YB2 [25,39].…”

Section: Temporal and Spatial Expression Patterns Of Ptnf-yb1 Genementioning

confidence: 99%

“…Studies in recent years have shown that individual NF-Y subunits in plants are involved in many important growth processes, especially in embryogenesis [11,12] and seed maturation [13][14][15], chloroplast synthesis [16][17][18], tissue division [19] and others processes. Simultaneously, the NF-Y subunit also plays an important role in response to stress, such as drought stress [20][21][22][23][24][25]. It is worth noting that more and more studies have found that the NF-Y subunit participates in the photoperiodic regulation of flowering induction pathways, and that different subunits function differently [26][27][28][29][30][31][32][33][34].…”

Section: Introductionmentioning

confidence: 99%

Genome-wide analysis of poplar NF-YB gene family and identified PtNF-YB1 important in regulate flowering timing in transgenic plants

et al. 2019

View full text Add to dashboard Cite

Background: Compared with annual herbaceous plants, woody perennials require a longer period of juvenile phase to flowering, and many traits can be only expressed in adulthood, which seriously makes the breeding efficiency of new varieties slower. For the study of poplar early flowering, the main focus is on the study Arabidopsis homologue gene CO/FT. Based on studies of Arabidopsis, rice and other plant species, some important research progress has been made on the regulation of flowering time by NF-Y subunits. However, little is known about the function of NF-Y regulating flowering in poplar. Results: In the present study, we have identified PtNF-YB family members in poplar and focus on the function of the PtNF-YB1 regulate flowering timing using transgenic Arabidopsis and tomato. To understand this mechanisms, the expression levels of three known flowering genes (CO, FT and SOC1) were examined with RT-PCR in transgenic Arabidopsis. We used the Y2H and BiFC to assay the interactions between PtNF-YB1 and PtCO (PtCO1 and PtCO2) proteins. Finally, the potential molecular mechanism model in which PtNF-YB1 play a role in regulating flowering in poplar was discussed. Conclusions: In this study, we have characterized the poplar NF-YB gene family and confirmed the function of the PtNF-YB1 regulate flowering timing. At the same time, we found that the function of PtNF-YB1 to improve early flowering can overcome species barriers. Therefore, PtNF-YB1 can be used as a potential candidate gene to improve early flowering by genetic transformation in poplar and other crops.

show abstract

Overexpression of StNF-YB3.1 reduces photosynthetic capacity and tuber production, and promotes ABA-mediated stomatal closure in potato ( Solanum tuberosum L.)

Cited by 21 publications

References 74 publications

Genes Encoding Transcription Factors TaDREB5 and TaNFYC-A7 Are Differentially Expressed in Leaves of Bread Wheat in Response to Drought, Dehydration and ABA

Genes Encoding Transcription Factors TaDREB5 and TaNFYC-A7 Are Differentially Expressed in Leaves of Bread Wheat in Response to Drought, Dehydration and ABA

Arabidopsis CK2 family gene CKB3 involved in abscisic acid signaling

Genome-wide analysis of poplar NF-YB gene family and identified PtNF-YB1 important in regulate flowering timing in transgenic plants

Contact Info

Product

Resources

About