Overexpression of Sp7 in odontoblasts results in dentinogenesis imperfecta due to the inhibition of odontoblast maturation

Miyazaki, Toshihiro; Inoue, Maaya; Baba, Tomomi T.; Komori, Toshihisa

doi:10.1016/j.job.2017.03.003

Cited by 4 publications

(1 citation statement)

References 22 publications

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“…This is once attachment bone has been fully laid down, dentinogenesis has been completed, and odontoblasts take on a flattened -interpreted as "resting"phenotype. Such a downregulation is also seen in maturing odontoblasts of mice (Miyazaki et al, 2017). Overall, expression data are to a large extent consistent with data for mice (Chen et al, 2009) although the expression appears to be more mesenchymespecific in zebrafish.…”

Section: Discussionsupporting

confidence: 83%

Zebrafish sp7 mutants show tooth cycling independent of attachment, eruption and poor differentiation of teeth

Kague

Witten

Soenens

et al. 2018

Developmental Biology

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The capacity to fully replace teeth continuously makes zebrafish an attractive model to explore regeneration and tooth development. The requirement of attachment bone for the appearance of replacement teeth has been hypothesized but not yet investigated. The transcription factor sp7 (osterix) is known in mammals to play an important role during odontoblast differentiation and root formation. Here we study tooth replacement in the absence of attachment bone using sp7 zebrafish mutants. We analysed the pattern of tooth replacement at different stages of development and demonstrated that in zebrafish lacking sp7, attachment bone is never present, independent of the stage of tooth development or fish age, yet replacement is not interrupted. Without bone of attachment we observed abnormal orientation of teeth, and abnormal connection of pulp cavities of predecessor and replacement teeth. Mutants lacking sp7 show arrested dentinogenesis, with non-polarization of odontoblasts and only a thin layer of dentin deposited. Osteoclast activity was observed in sp7 mutants; due to the lack of bone of attachment, remodelling was diminished but nevertheless present along the pharyngeal bone. We conclude that tooth replacement is ongoing in the sp7 mutant despite poor differentiation and defective attachment. Without bone of attachment tooth orientation and pulp organization are compromised.

show abstract

Section: Discussionsupporting

confidence: 83%

Zebrafish sp7 mutants show tooth cycling independent of attachment, eruption and poor differentiation of teeth

Kague

Witten

Soenens

et al. 2018

Developmental Biology

View full text Add to dashboard Cite

show abstract

SP7: from Bone Development to Skeletal Disease

Wang

Tokavanich

Wein

2023

Curr Osteoporos Rep

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Zebrafish sp7 mutants show tooth cycling independent of attachment, eruption and poor differentiation of teeth

Kague

Witten

Soenens

et al. 2018

Preprint

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2 SummaryThe capacity to fully replace teeth continuously makes zebrafish an attractive model to explore regeneration and tooth development. The requirement of attachment bone for the appearance of replacement teeth has been hypothesized but not yet investigated. The transcription factor sp7 (osterix) is known in mammals to play an important role during odontoblast differentiation and root formation. Here we study tooth replacement in the absence of attachment bone using sp7 zebrafish mutants. We analysed the pattern of tooth replacement at different stages of development and demonstrated that in zebrafish lacking sp7, attachment bone is never present, independent of the stage of tooth development or fish age, yet replacement is not interrupted. Without bone of attachment we observed abnormal orientation of teeth, and abnormal connection of pulp cavities of predecessor and replacement teeth. Mutants lacking sp7 show arrested dentinogenesis, with non-polarization of odontoblasts and only a thin layer of dentin deposited. Osteoclast activity was observed in sp7 mutants; due to the lack of bone of attachment, remodelling was diminished but nevertheless present along the pharyngeal bone. We conclude that tooth replacement is ongoing in the sp7 mutant despite poor differentiation and defective attachment. Without bone of attachment tooth orientation and pulp organization are compromised.

show abstract

Overexpression of Sp7 in odontoblasts results in dentinogenesis imperfecta due to the inhibition of odontoblast maturation

Cited by 4 publications

References 22 publications

Zebrafish sp7 mutants show tooth cycling independent of attachment, eruption and poor differentiation of teeth

Zebrafish sp7 mutants show tooth cycling independent of attachment, eruption and poor differentiation of teeth

SP7: from Bone Development to Skeletal Disease

Zebrafish sp7 mutants show tooth cycling independent of attachment, eruption and poor differentiation of teeth

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