Overexpression of SmANS Enhances Anthocyanin Accumulation and Alters Phenolic Acids Content in Salvia miltiorrhiza and Salvia miltiorrhiza Bge f. alba Plantlets

Li, Hongyan; Liu, Jingling; Pei, Tianlin; Bai, Zhenqing; Han, Ru; Liang, Zongsuo

doi:10.3390/ijms20092225

Cited by 39 publications

(23 citation statements)

References 60 publications

(92 reference statements)

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“…The suppressed expression of the ANS gene leads to a lack of anthocyanins in Caryophyllales [ 35 ]. Li et al [ 36 ] reported that overexpression of SmANS increased anthocyanin content but reduced the biosynthesis of salvianolic acid B in S. miltiorrhiza. White-flowered S. miltiorrhiza is a variety of the S. miltiorrhiza species.…”

Section: Discussionmentioning

confidence: 99%

Integrated metabolomic and transcriptomic analysis of the anthocyanin regulatory networks in Salvia miltiorrhiza Bge. flowers

et al. 2020

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Background The objectives of this study were to reveal the anthocyanin biosynthesis metabolic pathway in white and purple flowers of Salvia miltiorrhiza using metabolomics and transcriptomics, to identify different anthocyanin metabolites, and to analyze the differentially expressed genes involved in anthocyanin biosynthesis. Results We analyzed the metabolomics and transcriptomics data of S. miltiorrhiza flowers. A total of 1994 differentially expressed genes and 84 flavonoid metabolites were identified between the white and purple flowers of S. miltiorrhiza . Integrated analysis of transcriptomics and metabolomics showed that cyanidin 3,5-O-diglucoside, malvidin 3,5-diglucoside, and cyanidin 3-O-galactoside were mainly responsible for the purple flower color of S. miltiorrhiza. A total of 100 unigenes encoding 10 enzymes were identified as candidate genes involved in anthocyanin biosynthesis in S. miltiorrhiza flowers. Low expression of the ANS gene decreased the anthocyanin content but enhanced the accumulation of flavonoids in S. miltiorrhiza flowers. Conclusions Our results provide valuable information on the anthocyanin metabolites and the candidate genes involved in the anthocyanin biosynthesis pathways in S. miltiorrhiza .

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Section: Discussionmentioning

confidence: 99%

Integrated metabolomic and transcriptomic analysis of the anthocyanin regulatory networks in Salvia miltiorrhiza Bge. flowers

et al. 2020

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“…Moreover, salvianolic acid B has a high concentration in the liver and a transporter known as OATP1B1 is involved in its hepatic uptake and clearance [22]. In general, flavonoids are implicated in plant coloration and stress resistance [23].…”

Section: Introductionmentioning

confidence: 99%

Inhibition of alpha-synuclein seeded fibril formation and toxicity by herbal medicinal extracts

Ardah

Ghanem

Abdulla

et al. 2020

BMC Complement Med Ther

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Background: Recent studies indicated that seeded fibril formation and toxicity of α-synuclein (α-syn) play a main role in the pathogenesis of certain diseases including Parkinson's disease (PD), multiple system atrophy, and dementia with Lewy bodies. Therefore, examination of compounds that abolish the process of seeding is considered a key step towards therapy of several synucleinopathies. Methods: Using biophysical, biochemical and cell-culture-based assays, assessment of eleven compounds, extracted from Chinese medicinal herbs, was performed in this study for their effect on α-syn fibril formation and toxicity caused by the seeding process. Results: Salvianolic acid B and dihydromyricetin were the two compounds that strongly inhibited the fibril growth and neurotoxicity of α-syn. In an in-vitro cell model, these compounds decreased the insoluble phosphorylated αsyn and aggregation. Also, in primary neuronal cells, these compounds showed a reduction in α-syn aggregates. Both compounds inhibited the seeded fibril growth with dihydromyricetin having the ability to disaggregate preformed α-syn fibrils. In order to investigate the inhibitory mechanisms of these two compounds towards fibril formation, we demonstrated that salvianolic acid B binds predominantly to monomers, while dihydromyricetin binds to oligomeric species and to a lower extent to monomers. Remarkably, these two compounds stabilized the soluble non-toxic oligomers lacking β-sheet content after subjecting them to proteinase K digestion. Conclusions: Eleven compounds were tested but only two showed inhibition of α-syn aggregation, seeded fibril formation and toxicity in vitro. These findings highlight an essential beginning for development of new molecules in the field of synucleinopathies treatment.

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“…The formula A 530 ‐0.25(A 657 ) was used to measure the absorbance values (A) of the extract at 530 and 657 nm. Three biological replicates per treatment were used to analyze anthocyanin levels as previously described (Li et al 2019).…”

Section: Methodsmentioning

confidence: 99%

“…A series of flavonoids are generated from 4‐coumaroyl‐CoA via pathways catalyzed by key enzymes such as chalcone synthase (CHS), chalcone isomerase (CHI), flavanone 3‐hydroxylase (F3H), dihydroflavonol reductase (DFR), anthocyanidin synthase (ANS), and others (Figure S2; Li et al 2019; Ni et al 2019). Identifying these biosynthetic pathway genes has laid the foundation for in‐depth genetic engineering studies.…”

Section: Introductionmentioning

confidence: 99%

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SmMYB2 promotes salvianolic acid biosynthesis in the medicinal herb Salvia miltiorrhiza

Deng

Wang

Huang

et al. 2020

JIPB

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MYB transcription factors play vital roles in plant growth and metabolism. The phytohormone methyl jasmonate (MeJA) promotes phenolic acid accumulation in the medicinal herb Salvia miltiorrhiza, but the regulatory mechanism is poorly understood. Here, we identified the MeJA-responsive R2R3-MYB transcription factor gene SmMYB2 from a transcriptome library produced from MeJAtreated S. miltiorrhiza hairy roots. SmMYB2 expression was tightly correlated with the expression of key salvianolic acid biosynthetic genes including CYP98A14. SmMYB2 was highly expressed in the periderm of S. miltiorrhiza and SmMYB2 localized to the nucleus. Overexpressing SmMYB2 in S. miltiorrhiza hairy roots significantly increased the levels of salvianolic acids (including rosmarinic acid and salvianolic acid B) by upregulating salvianolic acid biosynthetic genes such as CYP98A14. SmMYB2 binds to the MYB-binding motifs in the promoter of CYP98A14, as confirmed by a dual-luciferase assay and electrophoretic mobility shift assays. Anthocyanin contents were significantly higher in SmMYB2-overexpressing hairy root lines than the control, primarily due to the increased expression of CHI, DFR, and ANS. These findings reveal the novel regulatory role of SmMYB2 in MeJA-mediated phenolic acid biosynthesis, providing a useful target gene for metabolic engineering and shedding light on the salvianolic acid regulatory network.

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Overexpression of SmANS Enhances Anthocyanin Accumulation and Alters Phenolic Acids Content in Salvia miltiorrhiza and Salvia miltiorrhiza Bge f. alba Plantlets

Cited by 39 publications

References 60 publications

Integrated metabolomic and transcriptomic analysis of the anthocyanin regulatory networks in Salvia miltiorrhiza Bge. flowers

Integrated metabolomic and transcriptomic analysis of the anthocyanin regulatory networks in Salvia miltiorrhiza Bge. flowers

Inhibition of alpha-synuclein seeded fibril formation and toxicity by herbal medicinal extracts

SmMYB2 promotes salvianolic acid biosynthesis in the medicinal herb Salvia miltiorrhiza

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