Overexpression of SlMBP22 in Tomato Affects Plant Growth and Enhances Tolerance to Drought Stress

Li, Fenfen; Chen, Xinyu; Zhou, Shengen; Xie, Qiaoli; Wang, Yunshu; Xiang, Xiaoxue; Hu, Zongli; Chen, Guoping

doi:10.1016/j.plantsci.2020.110672

Cited by 21 publications

(12 citation statements)

References 108 publications

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“…The ethylene-responsive gene ER5, ascorbate peroxidase gene APX2, GDP-mannose 3 ,5 -epimerase gene GME2, and catalase gene CAT3 were plant stress-related genes [42][43][44]. When transgenic plants become more resistant to drought, the expression of these genes is upregulated in general [45]. Here, after PEG6000 treatment for 24 h, transcript levels of ER5, CAT3, APX2, and GME2 were downregulated in SlADK10silenced plants compared to those in WT plants, which further confirmed the positive regulatory role of SlADK10 in drought response.…”

Section: Discussionmentioning

confidence: 99%

Genome-Wide Identification and Expression Analysis of Tomato ADK Gene Family during Development and Stress

Lü

Cao

Zhu

et al. 2021

IJMS

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Adenylate kinase (ADK) is widely distributed in organisms and plays an important role in cellular energy homeostasis. In plants, ADK has important functions in plant growth and development regulation as well as in adaptation to the environment. However, little information is available about the ADK genes in tomato (Solanum lycopersicum), an important economic crop. To investigate the characteristics and functions of ADK genes in tomato, a total of 11 ADK genes were identified and named according to their chromosomal locations. The ADK family in Arabidopsis, tomato, potato, and rice was divided into six groups, and motif analysis revealed that each SlADK protein contained five to eight conserved motifs. A total of 4 to 19 exons were identified in tomato ADK gene family members, and interestingly, most members possessed 4 exons. Several stress response elements were identified in the promoter regions of SlADKs. The 11 SlADKs were randomly distributed on 9 of the 12 tomato chromosomes. Three duplication events were observed between tomato chromosomes, and a high degree of conservation of synteny was demonstrated between tomato and potato. The online TomExpress platform prediction revealed that SlADKs were expressed in various tissues and organs, basically consistent with the data obtained from real-time quantitative PCR (qPCR). The qPCR verification was also performed to determine the expression level of SlADKs and demonstrated that the genes responded to multiple abiotic stresses, such as drought, salt, and cold. Besides, the qPCR results showed that SlADK transcription was responsive to most of the applied hormone treatment. For correlation network analysis under 44 global conditions, the results showed that the number of 17, 3, 4, and 6 coexpressed genes matched with SlADK5, 8, 9, and 11, respectively. For specific gene function analysis, expression of SlADK10 was inhibited using virus-induced gene silencing (VIGS). Compared to wild-type plants, plants with silenced SlADK10 gene had poor drought resistance, indicating SlADK10 regulated drought tolerance of tomato positively. In summary, the information provided in the present study will be helpful to understand the evolutionary relationship and their roles of tomato ADK gene family in further research.

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Section: Discussionmentioning

confidence: 99%

Genome-Wide Identification and Expression Analysis of Tomato ADK Gene Family during Development and Stress

Lü

Cao

Zhu

et al. 2021

IJMS

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“…CpMADS3 in cyclamen had a novel function in flowering [ 59 ]. Furthermore, MADS genes participate in multiple abiotic stress responses [ 60 , 61 , 62 ].…”

Section: Discussionmentioning

confidence: 99%

Genome-Wide Analysis and the Expression Pattern of the MADS-Box Gene Family in Bletilla striata

Zhao

et al. 2021

Plants

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Bletilla striata (Thunb. ex A. Murray) Rchb. f., a species of the perennial herb Orchidaceae, has potent anti-inflammatory and antiviral biological activities. MADS-box transcription factors play critical roles in the various developmental processes of plants. Although this gene family has been extensively investigated in many species, it has not been analyzed for B. striata. In total, 45 MADS-box genes were identified from B. striata in this study, which were classified into five subfamilies (Mδ, MIKC, Mα, Mβ, and Mγ). Meanwhile, the highly correlated protein domains, motif compositions, and exon–intron structures of BsMADSs were investigated according to local B. striata databases. Chromosome distribution and synteny analyses revealed that segmental duplication and homologous exchange were the main BsMADSs expansion mechanisms. Further, RT-qPCR analysis revealed that BsMADSs had different expression patterns in response to various stress treatments. Our results provide a potential theoretical basis for further investigation of the functions of MADS genes during the growth of B. striata.

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“…Tomato ( Solanum lycopersicum cv. Ailsa Craig), Nr and rin plants were grown under glasshouse conditions as described in our previous report [ 34 ]. Flowers were tagged at the stage of anthesis to assess fruit ripening stages.…”

Section: Methodsmentioning

confidence: 99%

A tomato HD-zip I transcription factor, VAHOX1, acts as a negative regulator of fruit ripening

Zhou

et al. 2022

Horticulture Research

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Homeodomain-leucine zipper (HD-Zip) transcription factors are only present in higher plants and are involved in plant development and stress responses. However, our understanding of their participation in the fruit ripening of economical plants, such as tomato (Solanum lycopersicum), remains largely unclear. Here, we report that VAHOX1, a member of the tomato HD-Zip I subfamily, was expressed in all tissues, was highly expressed in breaker+4 fruits, and could be induced by ethylene. RNAi repression of VAHOX1 (VAHOX1-RNAi) resulted in accelerated fruit ripening, enhanced sensitivity to ethylene, and increased total carotenoid content and ethylene production. Conversely, VAHOX1 overexpression (VAHOX1-OE) in tomato had the opposite effect. RNA-Seq results showed that altering VAHOX1 expression affected the transcript accumulation of a series of genes involved in ethylene biosynthesis and signal transduction and cell wall modification. Additionally, a dual-luciferase reporter assay, histochemical analysis of GUS activity and a yeast one-hybrid (Y1H) assay revealed that VAHOX1 could activate the expression of AP2a. Our findings may expand our knowledge about the physiological functions of HD-Zip transcription factors in tomato and highlight the diversities of transcriptional regulation during the fruit ripening process.

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Overexpression of SlMBP22 in Tomato Affects Plant Growth and Enhances Tolerance to Drought Stress

Cited by 21 publications

References 108 publications

Genome-Wide Identification and Expression Analysis of Tomato ADK Gene Family during Development and Stress

Genome-Wide Identification and Expression Analysis of Tomato ADK Gene Family during Development and Stress

Genome-Wide Analysis and the Expression Pattern of the MADS-Box Gene Family in Bletilla striata

A tomato HD-zip I transcription factor, VAHOX1, acts as a negative regulator of fruit ripening

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