Overexpression of signal sequence receptor γ predicts poor survival in patients with hepatocellular carcinoma

Huang, Shanzhou; Zhong, Wenqiang; Shi, Zhi; Wang, Kun; Jin, Huilin; Zhang, Zijian; Wang, Huanyu; Wei, Yingnan; Chen, Sixv; Zhou, Qi; He, Xiaoshun

doi:10.1016/j.humpath.2018.06.014

Cited by 14 publications

(18 citation statements)

References 21 publications

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“…Two cohorts of HCC patients were enrolled in our study. In cohort 1, formalin‐fixed, paraffin‐embedded HCC tissues and paired adjacent nontumor liver tissues (ANLT) from 95 HCC patients who initially underwent hepatic resection between July 2013 and December 2014 were used . ANLT was procured from disease livers at least 5 cm from the edge of tumor and verified by pathologist.…”

Section: Methodsmentioning

confidence: 99%

Uridine‐cytidine kinase 2 upregulation predicts poor prognosis of hepatocellular carcinoma and is associated with cancer aggressiveness

Huang

Tam

et al. 2019

Molecular Carcinogenesis

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Patients with advanced hepatocellular carcinoma (HCC) continue to have a dismal prognosis. Potential biomarkers to determine prognosis and select targeted therapies are urgently needed for patients with HCC. This study aimed to elucidate the role of UCK2 in HCC prognosis and tumor progression. We performed a screen of public databases to identify functional genes associated with HCC tumorigenesis, progression, and outcome. We identified uridine-cytidine kinase 2 (UCK2) as a gene of interest for further study. UCK2 promoting HCC aggressiveness was demonstrated by evaluation of clinical samples, in vitro experiments, in vivo tumorigenicity, and transcript analysis. UCK2 expression was generally elevated in HCC and was significantly correlated with poor survival and inferior clinicopathological characteristics of HCC patients. A multivariate analysis revealed that high UCK2 expression was an independent factor for poor prognosis. In HCC cell lines, UCK2 knockdown suppressed cell migration and invasion and inhibited cell proliferation, while UCK2 overexpression had an opposite effect. Animal model experiments confirmed that knockdown of UCK2 suppressed tumor growth in vivo.The bioinformatics analysis demonstrated that UCK2 might associated with metabolsim, splicesome, and adherens junction. UCK2 is highly associated with HCC malignant behavior and is a potential prognostic predictor for HCC patients in the clinic.

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Section: Methodsmentioning

confidence: 99%

Uridine‐cytidine kinase 2 upregulation predicts poor prognosis of hepatocellular carcinoma and is associated with cancer aggressiveness

Huang

Tam

et al. 2019

Molecular Carcinogenesis

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“…Total RNA was extracted from approximately 100 mg of tissue using the Qiagen RNeasy Mini Kit according to the manufacturer's instructions as previously described. 23 To detect GIHCG expression in HCC and ANTs, Quantitative real-time PCR (qRT-PCR) was performed using the Power SYBR Green PCR Master Mix (Applied Biosystems, USA) according to the manufacturer's instructions with gene-specific primers. Primers were designed as follows:…”

Section: Rna Extraction and Gihcg Expression Measurementmentioning

confidence: 99%

<p>Overexpression of <em>GIHCG</em> is Associated with a Poor Prognosis and Immune Infiltration in Hepatocellular Carcinoma</p>

Xiao¹,

Huang

Yang

2020

OTT

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Purpose Hepatocellular carcinoma (HCC) is one of the most commonly diagnosed digestive cancers and the fourth leading cause of death worldwide. Long noncoding RNAs (lncRNAs) with key roles in HCC development and progression have emerged and are used in the diagnosis and prognostic prediction of HCC. The lncRNA gradually increased during hepatocarcinogenesis ( GIHCG ) is a novel lncRNA with aberrant expression in many tumors, but its prognostic value and biological role in HCC have not been fully studied. Thus, the aim of this study was to explore the expression pattern and potential biological role of GIHCG in HCC. Patients and Methods The expression pattern of GIHCG in HCC was analyzed in our HCC cohort and validated in The Cancer Genome Atlas (TCGA) database. To assess the prognostic value of GIHCG , survival analyses and Cox regression analyses were carried out in two HCC cohorts. Functional enrichment analysis was used to predict the gene sets and pathways related to aberrant GIHCG expression. Furthermore, the relationship between GIHCG expression and immune infiltration in HCC was analyzed. Results GIHCG was highly expressed in HCC tissues compared with normal liver tissues. In addition, high GIHCG expression was significantly correlated with inferior clinicopathological characteristics and shorter survival times. High GIHCG expression was an independent prognostic factor for overall survival and disease-free survival in the HCC cohort in our center and in the TCGA-LIHC cohort. Hallmark terms such as “G2M checkpoint”, “ MYC targets” and “DNA repair” were enriched in the GIHCG high-expression groups. High GIHCG expression negatively correlated with the infiltration of memory CD4+ and CD8+ T cells, natural killer cells, macrophages, dendritic cells, neutrophils and monocytes. Conclusion The findings of our study indicate that GIHCG is a biomarker that can be used to predict the prognosis of HCC patients and is correlated with immune cell infiltration in HCC.

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“…SSR3 acts as a novel oncogene in hepatocellular carcinoma (HCC) and therefore can serve as a biomarker for the prognosis of HCC patients. [ 22 ] However, there are no reports of a relationship between this gene and hematological disorders. Further experiments are needed to confirm whether SSR3 is related with the poor prognosis of the present case.…”

Section: Discussionmentioning

confidence: 99%

Identification of a cryptic submicroscopic deletion using a combination of fluorescence in situ hybridization and array comparative genomic hybridization in a t(3;5)(q25;q35)-positive acute myeloid leukemia patient

Gao

Wang

et al. 2020

Medicine

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Rationale: The advent of high-resolution genome arrays including array comparative genomic hybridization (aCGH) has enabled the detection of cryptic submicroscopic deletions flanking translocation breakpoints in up to 20% of the apparently “balanced” structural chromosomal rearrangements in hematological disorders. However, reports of submicroscopic deletions flanking the breakpoints of t(3;5)(q25;q35) are rare and the clinical significance of submicroscopic deletions in t(3;5) has not been explicitly identified. Patient concerns: We present a 47-year-old man with acute myeloid leukemia. G-banding analysis identified t(3;5)(q25;q35). Diagnosis: Array CGH-based detection initially confirmed only the deletion of chromosome 3. Further characterization using fluorescence in situ hybridization identified a cryptic submicroscopic deletion including 5′ MLF1-3′ NPM1 flanking the breakpoint on the derivative chromosome 3. Interventions: The patient started “7+3” induction chemotherapy with cytosine arabinoside and daunorubicin, and subsequently received 2 cycles of high-dose intermittent acronym of cytosine arabinoside or cytarabine. Outcomes: The patient did not undergo complete remission and died from an infection due to neutropenia. Lessons: Haploinsufficiency of NPM1 or other deleted genes, including SSR3 , may be responsible for the phenotype of t(3;5)(q25;q35)-positive myeloid neoplasms with submicroscopic deletions.

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Overexpression of signal sequence receptor γ predicts poor survival in patients with hepatocellular carcinoma

Cited by 14 publications

References 21 publications

Uridine‐cytidine kinase 2 upregulation predicts poor prognosis of hepatocellular carcinoma and is associated with cancer aggressiveness

Uridine‐cytidine kinase 2 upregulation predicts poor prognosis of hepatocellular carcinoma and is associated with cancer aggressiveness

<p>Overexpression of <em>GIHCG</em> is Associated with a Poor Prognosis and Immune Infiltration in Hepatocellular Carcinoma</p>

Identification of a cryptic submicroscopic deletion using a combination of fluorescence in situ hybridization and array comparative genomic hybridization in a t(3;5)(q25;q35)-positive acute myeloid leukemia patient

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