Overexpression of SAPCD2 correlates with proliferation and invasion of colorectal carcinoma cells

Luo, Yongshi; Wang, Lili; Ran, Wenwen; Li, Guangqi; Xiao, Yujing; Wang, Xiaonan; Zhao, Han; Xing, Xiaoming

doi:10.1186/s12935-020-1121-6

Cited by 12 publications

(14 citation statements)

References 30 publications

(42 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…SAPCD2 (Suppressor anaphase-promoting complex domain containing 2) plays important roles in the initiation, invasion, and metastasis of several malignancies. Previous studies have shown that SAPCD2 expression was significantly higher in colon cancer tissues than in adenoma and normal epithelial tissues, and it significantly promoted cell proliferation, migration, and invasion both in vitro and in vivo [ 39 ]. SKA3 (spindle- and kinetochore-related complex subunit 3) is a component of the spindle- and kinetochore-related complexes and is essential for accurate timing of late mitosis.…”

Section: Discussionmentioning

confidence: 99%

Expression and Prognostic Characteristics of m6A RNA Methylation Regulators in Colon Cancer

Huang

Zhu

Kong

et al. 2021

IJMS

View full text Add to dashboard Cite

Colon cancer is a common and leading cause of death and malignancy worldwide. N6-methylation of adenosine (m6A) is the most common reversible mRNA modification in eukaryotes, and it plays a crucial role in various biological functions in vivo. Dysregulated expression and genetic changes of m6A regulators have been correlated with tumorigenesis, cancer cell proliferation, tumor microenvironment, and prognosis in cancers. This study used RNA-seq and colon cancer clinical data to explore the relationship between N6-methylation and colon cancer. Based on the seven m6A regulators related to prognosis, three molecular subgroups of colon cancer were identified. Surprisingly, we found that each subgroup had unique survival characteristics. We then identified three subtypes of tumors based on 299 m6A phenotype-related genes, and one subtype was characterized as an immunosuppressive tumor and patients in this subtype may be more suitable for immunotherapy than other subtypes. Finally, using m6A-related genes and clinical information from The Cancer Genome Atlas cohort, we constructed a prognosis model, and this model could be used to predict the prognosis of patients in clinics.

show abstract

Section: Discussionmentioning

confidence: 99%

Expression and Prognostic Characteristics of m6A RNA Methylation Regulators in Colon Cancer

Huang

Zhu

Kong

et al. 2021

IJMS

View full text Add to dashboard Cite

show abstract

“…Since its discovery, SAPCD2 has attracted great interest regarding its role in the context of cancer studies. SAPCD2 has been reported to be upregulated in various kinds of tumors, including breast cancer (34), gastric cancer (11), hepatocellular carcinoma (17), colorectal carcinoma (14), glioblastoma (19), nasopharyngeal carcinoma (15), lung adenocarcinoma (16), melanoma (18), and renal cell cancer (20). SAPCD2 further promotes progression and metastasis in different human types of cancer by activating the Wnt/b-catenin, JAK/STAT, TGF-b, MAPK, and NF-kB signaling pathways, all of which have been reported to play crucial roles in cancer progression and metastasis (35)(36)(37)(38)(39)(40).…”

Section: Discussionmentioning

confidence: 99%

“…SAPCD2 located on human chromosome 9q42.3 encodes a 389-amino acid protein (42.3 kDa) with EF-Hand domain at the N-terminal and functional CC-domains at the C-terminus (13). Accumulating studies have reported that SAPCD2 is overexpressed in several types of human cancer, and that SAPCD2 overexpression promotes the proliferation, migration, and invasion of cancer cells by various mechanisms (11,(14)(15)(16)(17)(18)(19)(20). However, little is known about the clinical significance and functional role of SAPCD2 in fibrosarcoma, especially metastatic fibrosarcoma.…”

Section: Introductionmentioning

confidence: 99%

Silencing SAPCD2 Represses Proliferation and Lung Metastasis of Fibrosarcoma by Activating Hippo Signaling Pathway

Zhu

Niu

et al. 2020

Front. Oncol.

View full text Add to dashboard Cite

The primary problem associated with fibrosarcoma is its high potential to metastasize to the lung. Aberrant expression of SAPCD2 has been widely reported to be implicated in the progression and metastasis in multiple cancer types. However, the clinical significance and biological roles of SAPCD2 in fibrosarcoma remain unknown. Here, we reported that SAPCD2 expression was markedly elevated in fibrosarcoma tissues, and its expression was differentially upregulated in fibrosarcoma cell lines compared with that in several primary fibroblast cell lines. Kaplan-Meier survival analysis revealed that SAPCD2 overexpression was significantly correlated with early progression and metastasis, and poor prognosis in fibrosarcoma patients. Our results further showed that silencing SAPCD2 inhibited the proliferation and increased the apoptosis of fibrosarcoma cells in vitro. Importantly, silencing SAPCD2 repressed lung metastasis of fibrosarcoma cells in vivo. Mechanistic investigation further demonstrated that silencing SAPCD2 inhibited the proliferation and lung metastasis of fibrosarcoma cells by activating the Hippo signaling pathway, as evidenced by the finding that constitutively active YAP1, YAP1-S127A, significantly reversed the inhibitory effect of SAPCD2 downregulation on the colony formation and anchorage-independent growth capabilities of fibrosarcoma cells, as well as the stimulatory effect on the apoptotic ratio of fibrosarcoma cells. In conclusion, SAPCD2 promotes the proliferation and lung metastasis of fibrosarcoma cells by regulating the activity of Hippo signaling, and this mechanism represents a potential therapeutic target for the treatment of lung metastatic fibrosarcoma.

show abstract

“…The percentage of positively stained tumor cells was scored as 0 (negative), 1 (1 − 25%), 2 (26–50%), 3 (51–75%), and 4 (>75%). The two scores were multiplied, and the resulting immune-reactive score (IRS) (values from 0 to 12) was used to classify the samples into two categories: high (4–12) and low (1–3) [ 23 ].…”

Section: Methodsmentioning

confidence: 99%

Molecular Hydrogen Inhibits Colorectal Cancer Growth via the AKT/SCD1 Signaling Pathway

Zhang

Tao

Zhao

et al. 2022

BioMed Research International

View full text Add to dashboard Cite

Objective. Molecular hydrogen (H2) has been considered a potential therapeutic target in many cancers. Therefore, we sought to assess the potential effect of H2 on colorectal cancer (CRC) in this study. Methods. The effect of H2 on the proliferation and apoptosis of RKO, SW480, and HCT116 CRC cell lines was assayed by CCK-8, colony formation, and flow cytometry assays. The effect of H2 on tumor growth was observed in xenograft implantation models (inhalation of 67% hydrogen two hours per day). Western blot and immunohistochemistry analyses were performed to examine the expression of p-PI3K, PI3K, AKT, pAKT, and SCD1 in CRC cell lines and xenograft mouse models. The expression of SCD1 in 491 formalin-fixed, paraffin-embedded CRC specimens was investigated with immunochemistry. The relationship between SCD1 status and clinicopathological characteristics and outcomes was determined. Results. Hydrogen treatment suppressed the proliferation of CRC cell lines independent of apoptosis, and the cell lines showed different responses to different doses of H2. Hydrogen also elicited a potent antitumor effect to reduce CRC tumor volume and weight in vivo. Western blot and IHC staining demonstrated that H2 inhibits CRC cell proliferation by decreasing pAKT/SCD1 levels, and the inhibition of cell proliferation induced by H2 was reversed by the AKT activator SC79. IHC showed that SCD1 expression was significantly higher in CRC tissues than in normal epithelial tissues (70.3% vs. 29.7%, p = 0.02 ) and was correlated with a more advanced TNM stage (III vs. I + II ; 75.9% vs. 66.3%, p = 0.02 ), lymph node metastasis (with vs. without; 75.9% vs. 66.3%, p = 0.02 ), and patients without a family history of CRC (78.7% vs. 62.1%, p = 0.047 ). Conclusion. This study demonstrates that high concentrations of H2 exert an inhibitory effect on CRC by inhibiting the pAKT/SCD1 pathway. Further studies are warranted for clinical evaluation of H2 as SCD1 inhibitor to target CRC.

show abstract

Overexpression of SAPCD2 correlates with proliferation and invasion of colorectal carcinoma cells

Cited by 12 publications

References 30 publications

Expression and Prognostic Characteristics of m6A RNA Methylation Regulators in Colon Cancer

Expression and Prognostic Characteristics of m6A RNA Methylation Regulators in Colon Cancer

Silencing SAPCD2 Represses Proliferation and Lung Metastasis of Fibrosarcoma by Activating Hippo Signaling Pathway

Molecular Hydrogen Inhibits Colorectal Cancer Growth via the AKT/SCD1 Signaling Pathway

Contact Info

Product

Resources

About