Overexpression of Rosa roxburghii l-galactono-1,4-lactone dehydrogenase in tobacco plant enhances ascorbate accumulation and abiotic stress tolerance

Liu, Wei; An, Hongyu; Yang, Minsheng

doi:10.1007/s11738-012-1204-7

Cited by 33 publications

(26 citation statements)

References 42 publications

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“…The increase in ascorbic acid content by potassium restriction was accompanied by an increase in GHLD activity ( Table 8). Previous studies showed that ascorbic acid accumulates when GHDL is upregulated [26,27]. Therefore, the increase in ascorbic acid content observed in this study may be attributable to the accumulation of the ascorbate matrix and the upregulation of GHDL during potassium restriction.…”

Section: Discussionsupporting

confidence: 55%

Cultivation Methods for Leafy Vegetables and Tomatoes with Low Potassium Content for Dialysis Patients and the Change of those Qualities

Ogawa¹

2018

Potassium - Improvement of Quality in Fruits and Vegetables Through Hydroponic Nutrient Management

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It is recommended that kidney disease patients receiving dialysis have limited potassium intake including intake of vegetables with high potassium content. Dialysis patients cannot absorb other nutrients contained in vegetables. To solve these problems, methods of cultivating vegetables with low potassium contents as compared to normal vegetables were studied. In leafy vegetables, the potassium contents were reduced as much with 60-70% by the cultivation without potassium applications during the latter half of the growth period compared with the controls, with no change in plant growth. In tomato, the potassium content in the potassium-restricted group was also reduced to 50-89% of the control. There was no change in the fresh weight per fruit of the tomatoes; however, the total yield was reduced. In this chapter, we introduce the researches of the cultivation method for leafy vegetables and tomatoes with low potassium content while still maintaining normal plant growth. Furthermore, the changes of the contents in minerals, ascorbic acid, and sugar in low potassium vegetables were reported.

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Section: Discussionsupporting

confidence: 55%

Cultivation Methods for Leafy Vegetables and Tomatoes with Low Potassium Content for Dialysis Patients and the Change of those Qualities

Ogawa¹

2018

Potassium - Improvement of Quality in Fruits and Vegetables Through Hydroponic Nutrient Management

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“…Exogenous application of AsA increases tolerance to drought, chilling, salinity and Al-induced oxidative damages in rice and tomato seedlings (Guo et al, 2005;Shatlata and Neumann, 2001). Up-regulation of AsA synthesis results in enhanced tolerance to salinity, high light-and MV-induced oxidative stress and aluminium toxicity (Chen et al, 2014;Liu et al, 2013). However, drought tolerance was not altered in ALO transgenic plants, which might be resulted from that the elevated AsA levels were not high enough to increase drought tolerance in ALO transgenic tobacco, or expression of some drought-and ABA-responsive genes, such as NtP5CS, NtTIP and NtERD10B, was not altered or even down-regulated by AsA.…”

Section: Discussionmentioning

confidence: 99%

“…Ascorbic acid is synthesized through multiple biosynthetic pathways in plants (Gallie, 2013), while the major pathway is Smirnoff-Wheeler pathway (Wheeler et al, 1998). Overexpression of D-galacturonic acid reductase, GDP-L-galactose phosphorylase, L-galactono-1,4lactone dehydrogenase and GDP-L-galactose guanyltransferase genes resulted in increased AsA levels (Agius et al, 2003;Bulley et al, 2009Bulley et al, , 2011Liu et al, 2013;Tokuna et al, 2005) and tolerance to paraquat and salinity stresses (Liu et al, 2013). D-Arabinono-1,4-lactone oxidase (ALO) is a key enzyme for synthesis of D-erythroascorbic acid in yeast.…”

Section: Introductionmentioning

confidence: 99%

Co‐expression of NCED and ALO improves vitamin C level and tolerance to drought and chilling in transgenic tobacco and stylo plants

Bao

Zhuo

Qian

et al. 2015

Plant Biotechnology Journal

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SummaryAbscisic acid (ABA) regulates plant adaptive responses to various environmental stresses, while Lascorbic acid (AsA) that is also named vitamin C is an important antioxidant and involves in plant stress tolerance and the immune system in domestic animals. Transgenic tobacco (Nicotiana tabacum L.) and stylo [Stylosanthes guianensis (Aublet) Swartz], a forage legume, plants coexpressing stylo 9-cis-epoxycarotenoid dioxygenase (SgNCED1) and yeast D-arabinono-1,4-lactone oxidase (ALO) genes were generated in this study, and tolerance to drought and chilling was analysed in comparison with transgenic tobacco overexpressing SgNCED1 or ALO and the wild-type plants. Compared to the SgNCED1 or ALO transgenic plants, in which only ABA or AsA levels were increased, both ABA and AsA levels were increased in transgenic tobacco and stylo plants co-expressing SgNCED1 and ALO genes. Compared to the wild type, an enhanced drought tolerance was observed in SgNCED1 transgenic tobacco plants with induced expression of drought-responsive genes, but not in ALO plants, while an enhanced chilling tolerance was observed in ALO transgenic tobaccos with induced expression of cold-responsive genes, but not in SgNCED1 plants. Co-expression of SgNCED1 and ALO genes resulted in elevated tolerance to both drought and chilling in transgenic tobacco and stylo plants with induced expression of both drought and cold-responsive genes. Our result suggests that co-expression of SgNCED1 and ALO genes is an effective way for use in forage plant improvement for increased tolerance to drought and chilling and nutrition quality.

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“…All leaf discs were incubated under a continuous white light intensity of 120 lmol m -2 s -1 . Phenotypic changes and chlorophyll content were measured to detect the effects of various treatments on leaf discs according to Liu et al (2013) and Alsaadawi et al (1986), respectively.…”

Section: Physiological Analysis Of Transgenic Plants and Abiotic Strementioning

confidence: 99%

Overexpression of Camellia sinensis H1 histone gene confers abiotic stress tolerance in transgenic tobacco

Wang

et al. 2014

Plant Cell Rep

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Overexpression of CsHis in tobacco promoted chromatin condensation, but did not affect the phenotype. It also conferred tolerance to low-temperature, high-salinity, ABA, drought and oxidative stress in transgenic tobacco. H1 histone, as a major structural protein of higher-order chromatin, is associated with stress responses in plants. Here, we describe the functions of the Camellia sinensis H1 Histone gene (CsHis) to illustrate its roles in plant responses to stresses. Subcellular localization and prokaryotic expression assays showed that the CsHis protein is localized in the nucleus, and its molecular size is approximately 22.5 kD. The expression levels of CsHis in C. sinensis leaves under various conditions were investigated by qRT-PCR, and the results indicated that CsHis was strongly induced by various abiotic stresses such as low-temperature, high-salinity, ABA, drought and oxidative stress. Overexpression of CsHis in tobacco (Nicotiana tabacum) promoted chromatin condensation, while there were almost no changes in the growth and development of transgenic tobacco plants. Phylogenetic analysis showed that CsHis belongs to the H1C and H1D variants of H1 histones, which are stress-induced variants and not the key variants required for growth and development. Stress tolerance analysis indicated that the transgenic tobacco plants exhibited higher tolerance than the WT plants upon exposure to various abiotic stresses; the transgenic plants displayed reduced wilting and senescence and exhibited greater net photosynthetic rate (Pn), stomatal conductance (Gs) and maximal photochemical efficiency (Fv/Fm) values. All the above results suggest that CsHis is a stress-induced gene and that its overexpression improves the tolerance to various abiotic stresses in the transgenic tobacco plants, possibly through the maintenance of photosynthetic efficiency.

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Overexpression of Rosa roxburghii l-galactono-1,4-lactone dehydrogenase in tobacco plant enhances ascorbate accumulation and abiotic stress tolerance

Cited by 33 publications

References 42 publications

Cultivation Methods for Leafy Vegetables and Tomatoes with Low Potassium Content for Dialysis Patients and the Change of those Qualities

Cultivation Methods for Leafy Vegetables and Tomatoes with Low Potassium Content for Dialysis Patients and the Change of those Qualities

Co‐expression of NCED and ALO improves vitamin C level and tolerance to drought and chilling in transgenic tobacco and stylo plants

Overexpression of Camellia sinensis H1 histone gene confers abiotic stress tolerance in transgenic tobacco

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