Overexpression of rice NAC gene SNAC1 in ramie improves drought and salt tolerance

An, Xiaopeng; Liao, Yu-Cai; Zhang, Jingyu; Dai, Lunjin; Zhang, Na; Wang, Bo; Liu, Lijun; Peng, Dingxiang

doi:10.1007/s10725-014-9991-z

Cited by 35 publications

(23 citation statements)

References 29 publications

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“…Some outstanding research progresses about Agrobacterium-mediated genetic transformation of ramie have been achieved. The corresponding research methods and influencing factors shall be discussed [65,66]. Furthermore, particle bombardment-mediated genetic transform is another effective way to realize transgenic plants.…”

Section: Resultsmentioning

confidence: 99%

Research Progress on Tissue Culture and Genetic Transformation of Kenaf (Hibiscus cannabinus)

Jin

Zhang

et al. 2017

Open Life Sciences

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Section: Resultsmentioning

confidence: 99%

Research Progress on Tissue Culture and Genetic Transformation of Kenaf (Hibiscus cannabinus)

Jin

Zhang

et al. 2017

Open Life Sciences

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“…For qRT-PCR, total RNA was extracted from 8week-old leaves and then reverse transcribed into cDNA. The ramie GAPDH gene was selected as an internal control and coamplified with the primers GAPDH forward (5 0 -T GGAAGAATCGGTAGGTTGG-3 0 ) and reverse (5 0 -GAC GCCAAAAACAGTGACAG-3 0 ) [33].…”

Section: Reverse Transcriptase-pcr Analysesmentioning

confidence: 99%

“…Six measurements were performed for chlorophyll content using a SPAD-502 plus (Konica Minolta, Tokyo, Japan) on corresponding functional leaves, and the average was taken. The photosynthesis rate was determined as previously described [33].…”

Section: Measurements Of Plant Height Stem Diameter Relative Chloromentioning

confidence: 99%

Senescence is delayed when ramie (Boehmeria nivea L.) is transformed with the isopentyl transferase (ipt) gene under control of the SAG12 promoter

Zhang

Liao

et al. 2017

FEBS Open Bio

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Ramie is an economically important industrial fiber crop widely planted in China, India, and other Southeast Asian and Pacific Rim countries. It plays an important role in China's economy, where ramie farming, industry, and trade provide livelihood support to about five million people. However, poor fiber production resulting from leaf senescence and leaf abscission is a significant problem. In this study, we report the successful production of transgenic ramie plants which delayed leaf senescence and enhanced biomass. Transgenic ramie plants were obtained via transformation with the Agrobacterium tumefaciens strain harboring the binary vector pSG529 containing the isopentyl transferase (ipt) gene under control of the SAG12 promoter (PSAG12‐ipt construct). Agrobacterium tumefaciens strain EHA105 was used for the midrib explant transformation. The transformation frequency was 28.29%. Southern blot confirmed the integration of 1–4 copies of the NPTII gene into the ramie genome in the tested lines. At the fiber maturation stage, the transgenic plants had higher photosynthesis rates, chlorophyll content (SPAD values), and stronger resistance to exogenous ethylene compared with wild‐type plants.

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“…Hundreds of transcription factors (TFs) have been found to modulate tolerance to various abiotic and biotic stresses [23][24][25][26]. Most of these TFs, which belong to several large TF families, such as AP2/ERF, bZIP, COL, MYB, HD-ZIP, and Recently, it was found that overexpression of SNAC1, a NAC gene known to regulate tolerance to drought in rice, remarkably improved tolerance to drought and excessive salinity in ramie [47]. This finding suggested that ramie likely possesses a regulatory stress tolerance network similar to that of rice.…”

Section: Comparison Of the Stress-responsive Tfs Between This Study Amentioning

confidence: 99%

Identification of drought, cadmium and root-lesion nematode infection stress-responsive transcription factors in ramie

et al. 2016

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Drought, cadmium (Cd) stress, and root lesion nematode (RLN) infection are three of the most important stresses affecting ramie growth and development; therefore, ramie breeding programs focus on their management more than on any other abiotic or biotic stresses. The fact that only a small number of stress-responsive transcription factors (TFs) have been identified so far is a major obstacle in the elucidation of mechanisms regulating the response to these three stresses in ramie. In this study, in order to uncover more stress-responsive TFs, a total of 179 nonredundant genes with full-length open reading frames from the MYB, AP2/ERF, bZIP, HD-ZIP, and COL families were obtained by searching for against the ramie transcriptome. Expression pattern analysis demonstrated that most of these genes showed relatively higher expression in the stem xylem and bast than in other tissues. Among these genes, 96 genes were found to be involved in responses to drought, Cd exposure, or RLN-infection. The expression of 54 of these genes was regulated by at least two stresses. These stress-responsive TFs probably have roles in the regulation of stress tolerance. The discovery of these stress-responsive TFs will be helpful for furthering our understanding of the mechanisms that regulate stress responses in ramie.

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Overexpression of rice NAC gene SNAC1 in ramie improves drought and salt tolerance

Cited by 35 publications

References 29 publications

Research Progress on Tissue Culture and Genetic Transformation of Kenaf (Hibiscus cannabinus)

Research Progress on Tissue Culture and Genetic Transformation of Kenaf (Hibiscus cannabinus)

Senescence is delayed when ramie (Boehmeria nivea L.) is transformed with the isopentyl transferase (ipt) gene under control of the SAG12 promoter

Identification of drought, cadmium and root-lesion nematode infection stress-responsive transcription factors in ramie

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