Overexpression of RhoA promotes the proliferation and migration of cervical cancer cells

Abstract

The pro-oncogenic role of RhoA has been well identified in other cancers, but rarely in cervical cancer (CC), one of the main causes of cancer-related death in women. In the present study, we identified the overexpression of RhoA and its downstream effectors, ROCK-1 and ROCK-II, in CC specimens using western blotting. Then, we determined the effect of RhoA on the proliferation and migration of Hela cells, one of CC cell lines, by upregulating or downregulating the RhoA expression in Hela cells. We found that there was an overexpression of RhoA, ROCK-I/II in CC, which was associated with the progression of CC. And we confirmed that RhoA promoted the proliferation and migration of CC cells. In conclusion, we found a positive correlation among RhoA with the progression of CC by in vivo and in vitro evidences. A high RhoA expression in CC may predict a high metastatic potential of CC.

Honokiol inhibits migration of renal cell carcinoma through activation of RhoA/ROCK/MLC signaling pathway

“…based on a number of studies, upregulated RhoA is associated with tumor progression in different types of cancer (33)(34)(35) and RhoA activation promotes the migration of cervical, colon and hepatocellular carcinoma (36)(37)(38). However, significantly downregulated expression of RhoA was demonstrated in human conventional RCC compared to that in normal kidney tissues (17) and activated RhoA has specifically been shown to inhibit the migration of breast and prostate cancer (39,40).…”

Metformin Inhibit Cervical Cancer Migration by Suppressing the FAK/Akt Signaling Pathway

“…The phosphorylated FAK and Akt trigger its downstream effectors including Rho families (Zhao et al, 2011). The Rac1 and RhoA-GTP downstream migratory protein controls the migration of cell by activating the development of cell-membrane protrusion filopodia at the boundary of the migrating side (Vega and Ridley, 2008;Liu et al, 2014). During the movement of cells, filopodia and lamellipodia increase and are required in migration and metastasis of cancer (Machesky, 2008;Dirat et al, 2014).…”

“…Recently, the Rho family of small guanosine triphosphatases (GTPases), has been reported to play a crucial role in reorganization of actin and the formation of filopodia. The expression level of Rac1 and RhoA were found to be increased in several cancers including cervical cancer (Kamai et al, 2004;Liu et al, 2014). Upon the activation of Rac1 and RhoA, cancer cells migration are enlarged (Vega et al, 2008;Liu et al, 2014).…”

siRNAs encapsulated in recombinant capsid protein derived from Dengue serotype 2 virus inhibits the four serotypes of the virus and proliferation of cancer cells

“…Using HiPerfect (Qiagen) as the transfection reagent, we have shown that siRNA 52 mediated Rho A knockdown results in the inhibition of actin stress fiber formation in endothelial (Huvec) cells (Kumar et al, 2011). Rho A is also known to be important in the proliferation of cervical cancer cells (Liu et al, 2014). In two other studies reported from our laboratory, siRNA 26 targeting Aurorakinase B (AURK-B) in the prostate cancer cell line, PC3 (Addepalli et al, 2010), and the hepatocellular carcinoma cell line, HepG2 , was reported.…”