Overexpression of Rho GDP-Dissociation Inhibitor Alpha Is Associated with Tumor Progression and Poor Prognosis of Colorectal Cancer

Zhao, Liang; Wang, Huiyun; Li, Jianming; Liu, Yawei; Ding, Yan‐Qing

doi:10.1021/pr800271b

Cited by 72 publications

(63 citation statements)

References 44 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Firstly, it is interesting to notice that the higher expression of proteins stimulating cell proliferation (CCNH, GDIR1 and MIF) [32][33][34] is detected in the 8505C cells showing higher growth rates than the BCPAP cells. Conversely a lower expression of proteins belonging to the folding control machinery may testify an impaired capability of the 8505C cells to perform differentiated cellular functions.…”

Section: Discussionmentioning

confidence: 99%

Differential proteomic and phenotypic behaviour of papillary and anaplastic thyroid cell lines

Musso

Cara

Albanese

et al. 2013

Journal of Proteomics

View full text Add to dashboard Cite

Thyroid carcinomas account for a minority of all malignant tumours but, after those of the gonads, they represent the most common forms of endocrine cancers. They include several types, among which the papillary thyroid cancer (PTC) and the anaplastic thyroid cancer (ATC) are the best known. The two hystotypes display significant biological and clinical differences: PTC is a well differentiated form of tumour with a high incidence and a good prognosis, while the ATC is less frequent but represents one of the most aggressive endocrine tumours with morphological features of an undifferentiated type. To date, as far as we know, no conclusive studies, useful to design arrays of molecular markers, have been published illustrating the phenotypic and proteomic differences between these two tumours. The aim of this work was to perform a comparative analysis of two thyroid cancer cell lines, derived respectively from papillary (BCPAP) and anaplastic (8505C) thyroid carcinomas. The comparative analysis included cell behaviour assays and proteomic analysis by 2D-PAGE and mass spectrometry. IntroductionThe human thyroid gland is composed of a basic structural unit, the follicle, consisting of a monolayer of well polarized cells, the thyrocytes, responsible for the T3/T4 hormone secretion, and of other peripheral cells, the parafollicular C cells, responsible for the secretion of calcitonin. The presence within the follicle of stem cells, or remnants of embryonic cells, has been hypothesized as the target cells for tumour initiation. A thin extracellular matrix, which includes occasional fibroblasts and inflammatory cells, is peripheral to the follicle structure. Thyroid carcinomas account for 1-2% of all malignant tumours and, after those of the gonads, they represent the most common tumours of the endocrine system. The thyroid

show abstract

Section: Discussionmentioning

confidence: 99%

Differential proteomic and phenotypic behaviour of papillary and anaplastic thyroid cell lines

Musso

Cara

Albanese

et al. 2013

Journal of Proteomics

View full text Add to dashboard Cite

show abstract

“…As previously described, IHC was performed (12) to investigate the expression of proteins inhuman colorectal cancer tissues. The sections were incubated overnight using primary antibodies against LASP1, S100A4 (1:500), E-cadherin, b-catenin, vimentin (1:50) at 4 C. Mayer's hematoxylin was used for the purpose of nuclear counter staining.…”

Section: Immunohistochemistrymentioning

confidence: 99%

LIM and SH3 Protein 1 Induces TGFβ-Mediated Epithelial–Mesenchymal Transition in Human Colorectal Cancer by Regulating S100A4 Expression

Wang

Shi

Luo

et al. 2014

Clinical Cancer Research

Self Cite

View full text Add to dashboard Cite

Purpose: The expression of LIM and SH3 protein 1 (LASP1) was upregulated in colorectal cancer cases, thereby contributing to the aggressive phenotypes of colorectal cancer cells. However, we still cannot decipher the underlying molecular mechanism associated with colorectal cancer metastasis.Experimental Design: In this study, IHC was performed to investigate the expression of proteins in human colorectal cancer tissues. Western blot analysis was used to assess the LASP1-induced signal pathway. Two-dimensional difference gel electrophoresis was performed to screen LASP1-modulated proteins and uncover the molecular mechanism of LASP1. TGFb was used to induce an epithelial-mesenchymal transition (EMT).Results: LASP1 expression was correlated with the mesenchymal marker vimentin and was inversely correlated with epithelial markers, namely, E-cadherin and b-catenin, in clinical colorectal cancer samples. The gain-and loss-of-function assay showed that LASP1 induces EMT-like phenotypes in vitro and in vivo. S100A4, identified as a LASP1-modulated protein, was upregulated by LASP1. Moreover, it is frequently coexpressed with LASP1 in colorectal cancer. S100A4 was required for EMT, and an increased cell invasiveness of colorectal cancer cell is induced by LASP1. Furthermore, the stimulation of TGFb resulted in an activated Smad pathway that increased the expression of LASP1 and S100A4. The depletion of LASP1 or S100A4 expression inhibited the TGFb signaling pathway. Moreover, it significantly weakened the proinvasive effects of TGFb on colorectal cancer cells.Conclusion: These findings elucidate the central role of LASP1 in the TGFb-mediated EMT process and suggest a potential target for the clinical intervention in patients with advanced colorectal cancer. Clin Cancer Res; 20(22); 5835-47. Ó2014 AACR.

show abstract

“…The tissues were embedded and cut into 4-µm sections. Immunohistochemistry was performed according to the protocol as previously described (10). The sections were incubated with the primary antibodies [rabbit anti-Fas-associated protein with death domain (FADD) antibody, Cell signaling technology (CST)] and biotinylated secondary antibody (Zymed Laboratories Inc., South San Francisco, CA, USA).…”

Section: Real-time Quantative Rt-pcrmentioning

confidence: 99%

Apigenin suppresses the growth of colorectal cancer xenografts via phosphorylation and up-regulated FADD expression

et al. 2010

View full text Add to dashboard Cite

Abstract. Apigenin is a flavonoid belonging to the flavone structural class. It has been implicated as a chemopreventive agent against prostate and breast cancers. However, to the best of our knowledge, no published data are available regarding apigenin in colorectal cancer (CRC). The effects and mechanisms of apigenin on CRC may vary significantly. This study aimed to analyze the effects of apigenin on the growth of CRC xenografts in nude mice derived from SW480, as well as to investigate the underlying mechanisms. Whole-body fluorescence imaging is an inexpensive optical system used to visualize gene expression in small mammals using reporter genes, such as eGFP as a reporter. In our study, the expression of eGFP may reflect the size of the tumor. A terminal deoxynucleotidyl transferase dUTP nick end-labeling (TUNEL) assay showed that apigenin promoted the apoptosis of CRC cells. Furthermore, the expression of five genes related to the proliferation and apoptosis of CRC, i.e., cyclin D1, BAG-1, Bcl-2, yrdC and Fas-associated protein with death domain (FADD), was detected by real-time quantitative RT-PCR. Among these genes, the up-regulated expression of FADD was noted in CRC xenograft tumors treated with apigenin. Immunohistochemistry and Western blotting confirmed the results at the protein level. Furthermore, Western blot analysis showed that apigenin induced the phosphorylation of FADD. Our findings suggest that apigenin enhances the expression of FADD and induces its phosphorylation, which may cause apoptosis of CRC cells and inhibition of tumor growth.

show abstract

Overexpression of Rho GDP-Dissociation Inhibitor Alpha Is Associated with Tumor Progression and Poor Prognosis of Colorectal Cancer

Cited by 72 publications

References 44 publications

Differential proteomic and phenotypic behaviour of papillary and anaplastic thyroid cell lines

Differential proteomic and phenotypic behaviour of papillary and anaplastic thyroid cell lines

LIM and SH3 Protein 1 Induces TGFβ-Mediated Epithelial–Mesenchymal Transition in Human Colorectal Cancer by Regulating S100A4 Expression

Apigenin suppresses the growth of colorectal cancer xenografts via phosphorylation and up-regulated FADD expression

Contact Info

Product

Resources

About