Overexpression of Regulatory T Cell-Related Markers (FOXP3, CTLA-4 and GITR) by Peripheral Blood Mononuclear Cells from Patients with Breast Cancer

Khalife, Esmat; Khodadadi, Ali; Talaeizadeh, Abdolhosein; Rahimian, Leila; Nemati, Maryam; Jafarzadeh, Abdollah

doi:10.31557/apjcp.2018.19.11.3019

Cited by 28 publications

(23 citation statements)

References 35 publications

(51 reference statements)

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“…These findings suggest that quantification of CD4+ Tregs may be valuable for assessing BC progression and as an important therapeutic target. In contrast, the frequency of CD4+ Tregs did not directly correlate with the clinical stage of breast cancer in other studies [14,28]. The aim of this study was to assess the frequency of CD4+ Tregs and CD8+ T cells in Egyptian women with BC in relation to high-risk HPV infection compared with healthy volunteers.…”

Section: Introductionmentioning

confidence: 80%

“…In addition, we analyzed the frequency of CD4+ Tregs among clinically defined surrogates of BC molecular subtypes including ER+, Her2+, and triple negative BC in order to explore whether any particular clinical disease correlates were associated with increased number of CD4+ Tregs. Increased CD4+ Tregs frequency was associated with poor prognostic characteristics such as higher histological grade, lymph node metastasis [14,34,35].…”

Section: Cd8+ T Lymphocytes and Cd4+ T Helper Cells May Promote Tumormentioning

confidence: 96%

“…We chacaterized CD4+ Tregs by the expression of CD4, CD25, and FOXP3 [14]. Representative plots of the gating and frequency of frequency of CD4+ Tregs are shown in Fig.…”

Section: Prevalence Of Cd4+ Tregsmentioning

confidence: 99%

“…Several subsets of regulatory CD4+ T cells (CD4+ Tregs) have been described in peripheral blood and tumor microenvironment from breast cancer patients with invasive high-grade breast carcinomas, and may play a key role in the progression of breast cancer expression [9][10][11][12][13]. CD4+ Tregs express high levels of FOX P3, CD25, tumor necrosis factor receptor-related protein (GITR), CTLA-4, and CD103 [14][15][16]. In addition, decreased expression of CD127 on human CD4+ Tregs is inversely correlated with FoxP3 expression [17,18].…”

Section: Introductionmentioning

confidence: 99%

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Role of CD4+ CD25+ FOXP3+ regulatory T-cells and human papilloma virus infection in Egyptian Women with breast cancer

Tawfeik

Mora

Osman

et al. 2019

Preprint

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Several subsets of regulatory CD4+ T cells (CD4+ Tregs) have been described in peripheral blood and tumor microenvironment and blood of breast cancer (BC) patients and may play a key role in the progression of BC. High-risk human papilloma virus (HPV) have a causal role in a significant proportion of cervical, and head, and neck tumors and may play an important role in evoking neoplasia in BC. In this study we assessed the prevalence of CD4+Tregs (CD4+CD25+ FOXP3+ cells) and CD8+T cells by flow cytometry in peripheral blood from a total of 55 Egyptian women, including 20 treatment-naïve BC, 15 with breast benign lesions (BBL) and 20 healthy volunteers (HV). High-risk HPV genotype type 16, 18, and 31 was investigated in breast tissue from all BC and BBL patients using Real-Time PCR. HPV was detected in 4 BC, but in none of BBL patients. The frequency of CD4+ Tregs was significantly higher in BC compared to BBL and HV, (p < 0.001). In addition, we observed a significantly higher frequency of CD8+ T cells in peripheral blood of patients with late stage III compared to early stage I and II BC (p = 0.011). However, there was no significant association between the ratio of CD8+ T cell to CD4+ Tregs frequencies and the expression of Estrogen Receptor (ER), Progesterone Receptor (PR), and Human Epidermal Growth Factor Receptor 2 (HER2). In conclusion, CD4+ Tregs may contribute to progression BC in Egyptian women with HPV infection. The potential role CD4+ Tregs as a prognostic or predictive parameter should be analyzed in a larger longitudinal study with sufficient follow-up time.

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Section: Introductionmentioning

confidence: 80%

Section: Cd8+ T Lymphocytes and Cd4+ T Helper Cells May Promote Tumormentioning

confidence: 96%

“…We chacaterized CD4+ Tregs by the expression of CD4, CD25, and FOXP3 [14]. Representative plots of the gating and frequency of frequency of CD4+ Tregs are shown in Fig.…”

Section: Prevalence Of Cd4+ Tregsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Role of CD4+ CD25+ FOXP3+ regulatory T-cells and human papilloma virus infection in Egyptian Women with breast cancer

Tawfeik

Mora

Osman

et al. 2019

Preprint

View full text Add to dashboard Cite

show abstract

Section: Cd8+ T Lymphocytes and Cd4+ T Helper Cells May Promote Tumormentioning

confidence: 96%

Association of CD4+ CD25+ FOXP3+ regulatory T-cells and human papilloma virus infection in Egyptian Women with breast cancer

Tawfeik

Mora

Osman

et al. 2020

Preprint

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Several subsets of regulatory CD4+ T cells (CD4+ Tregs) have been described in peripheral blood and tumor microenvironment and blood of breast cancer (BC) patients and may play a key role in the progression of BC. High-risk human papilloma virus (HPV) have a causal role in a significant proportion of cervical, and head, and neck tumors and may play an important role in evoking neoplasia in BC. In this study we assessed the prevalence of CD4+Tregs (CD4+CD25+ FOXP3+ cells) and CD3+ CD8+ T cells by flow cytometry in peripheral blood from a total of 55 Egyptian women, including 20 treatment-naïve BC, 15 with breast benign lesions (BBL) and 20 healthy volunteers (HV). High-risk HPV genotype type 16, 18, and 31 was investigated in breast tissue from all BC and BBL patients using Real-Time PCR. HPV was detected in 4 BC, but in none of BBL patients. The frequency of CD4+ Tregs was significantly higher in BC compared to BBL and HV, (p < 0.001). In addition, we observed a significantly higher frequency of CD3+ CD8+ T cells in peripheral blood of patients with late stage III compared to early stage I and II BC (p = 0.011). However, there was no significant association between the ratio of CD8+ T cell to CD4+ Tregs frequencies and the expression of Estrogen Receptor (ER), Progesterone Receptor (PR), and Human Epidermal Growth Factor Receptor 2 (HER2). In conclusion, CD4+ Tregs may contribute to progression BC in Egyptian women with HPV infection. The potential role CD4+ Tregs as a prognostic or predictive parameter should be analyzed in a larger longitudinal study with sufficient follow-up time.

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Prognostic and therapeutic role of tumor-infiltrating lymphocyte subtypes in breast cancer

Nelson

Ngamcherdtrakul

Luoh

et al. 2021

Cancer Metastasis Rev

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Increased levels of total tumor-infiltrating lymphocytes (TILs) are generally associated with good prognosis in several breast cancer subtypes. Subtypes of TILs impact both tumor cells and immune cells in a variety of different ways, leading to either a protumor or antitumor effect. Tumor-infiltrating CD8 + T cells and natural killer (NK) cells perform as effector cells against tumor cells and are associated with better clinical outcome. Immunotherapy approaches that improve the antitumor activity and proliferation of CD8 + T and NK cells include PD-1/PD-L1 blockade, CAR T cell therapy, or ex vivo-stimulated NK cells. A subset of CD8 + T cells, tissue-resident memory T cells, has also recently been associated with good prognosis in breast cancer patients, and has potential to serve as a predictive biomarker and therapeutic target. Tumor-infiltrating B cells also secrete apoptosis-inducing IgG antibodies and can act as antigen-presenting cells to prime CD4 + and CD8 + T cells. On the other hand, regulatory T and regulatory B cells modulate the immune response from CD8 + T cells and NK cells by secreting immunosuppressive cytokines and inhibiting maturation of antigen-presenting cells (APCs). These regulatory cells are typically associated with poor prognosis, therefore rendering suppression of their regulatory function a key immunotherapeutic strategy.

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Overexpression of Regulatory T Cell-Related Markers (FOXP3, CTLA-4 and GITR) by Peripheral Blood Mononuclear Cells from Patients with Breast Cancer

Cited by 28 publications

References 35 publications

Role of CD4+ CD25+ FOXP3+ regulatory T-cells and human papilloma virus infection in Egyptian Women with breast cancer

Role of CD4+ CD25+ FOXP3+ regulatory T-cells and human papilloma virus infection in Egyptian Women with breast cancer

Association of CD4+ CD25+ FOXP3+ regulatory T-cells and human papilloma virus infection in Egyptian Women with breast cancer

Prognostic and therapeutic role of tumor-infiltrating lymphocyte subtypes in breast cancer

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