Overexpression of PtoCYCD3;3 Promotes Growth and Causes Leaf Wrinkle and Branch Appearance in Populus

Guan, Chao‐Nan; Xue, Yuan; Jiang, Pengfei; He, Chengcheng; Zhuge, X.L.; Lan, Ting; Yang, Hai-Ling

doi:10.3390/ijms22031288

Cited by 16 publications

(18 citation statements)

References 45 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…CYCD3;1 plays a role in cell division and proliferation, and it was only differentially expressed in diploid and tetraploid young leaves, which indicates that a significant increase in mesophyll cells of the tetraploid mainly occurred in young leaves ( Dewitte et al, 2003 ; de Jager et al, 2009 ). In Populus , overexpressing CYCD3;1 could significantly increase the number of cells in leaves, but cell volume decreased ( Guan et al, 2021 ). However, the cell volume increased after tetraploidy in E. urophylla ( Figures 4D , 6F ).…”

Section: Discussionmentioning

confidence: 99%

Induction and Characterization of Tetraploid Through Zygotic Chromosome Doubling in Eucalyptus urophylla

Liu

Wang²,

Qiu³

et al. 2022

Front. Plant Sci.

View full text Add to dashboard Cite

Improvements in plant growth can bring great benefits to the forest industry. Eucalyptus urophylla is an important plantation species worldwide, and given that ploidy increases are often associated with plant phenotype changes, it was reasoned that its polyploidization may have good prospects and great significance toward its cultivation. In this study, the zygotic development period of E. urophylla was observed through paraffin sections, and a correlation between the development time of flower buds after pollination and the zygotic development period was established. On this basis, it was determined that the 25th day after pollination was the appropriate time for a high temperature to induce zygotic chromosome doubling. Then tetraploid E. urophylla was successfully obtained for the first time through zygotic chromosome doubling induced by high temperature, and the appropriate conditions were treating flower branches at 44°C for 6 h. The characterization of tetraploid E. urophylla was performed. Chromosome duplication brought about slower growing trees with thicker leaves, larger cells, higher net photosynthetic rates, and a higher content of certain secondary metabolites. Additionally, the molecular mechanisms for the variation in the tetraploid’s characteristics were studied. The qRT-PCR results showed that genes mediating the tetraploid characteristics showed the same change trend as those of the characteristics, which verified that tetraploid trait variation was mainly caused by gene expression changes. Furthermore, although the tetraploid had no growth advantage compared with the diploid, it can provide important germplasm resources for future breeding, especially for the creation of triploids.

show abstract

Section: Discussionmentioning

confidence: 99%

Induction and Characterization of Tetraploid Through Zygotic Chromosome Doubling in Eucalyptus urophylla

Liu

Wang²,

Qiu³

et al. 2022

Front. Plant Sci.

View full text Add to dashboard Cite

show abstract

“…After being co-expressed in the vascular cambium of Arabidopsis roots, Aintegumenta (ANT) and CYCD3;1 were essential for root secondary thickening in response to cytokinins [22]. PtoCYCD3;3 transgenic poplar showed enlarged and wrinkled leaves and thickened and elongated stems, resulting in the abnormal differentiation of cambium and stem [23]. During the adventitious root development phase, PeCYCD6;1 promoted the periclinal division and separated endodermis and cortex cell layers by association with the Short root (SHR)/Scarecrow (SCR) network [24].…”

Section: Introductionmentioning

confidence: 99%

Overexpression Populus d-Type Cyclin Gene PsnCYCD1;1 Influences Cell Division and Produces Curved Leaf in Arabidopsis thaliana

Zheng

Dai

Liu

et al. 2021

IJMS

View full text Add to dashboard Cite

d-type cyclins (CYCDs) are a special class of cyclins and play extremely important roles in plant growth and development. In the plant kingdom, most of the existing studies on CYCDs have been done on herbaceous plants, with few on perennial woody plants. Here, we identified a Populus d-type cyclin gene, PsnCYCD1;1, which is mainly transcribed in leaf buds and stems. The promoter of PsnCYCD1;1 activated GUS gene expression and transgenic Arabidopsis lines were strongly GUS stained in whole seedlings and mature anthers. Moreover, subcellular localization analysis showed the fluorescence signal of PsnCYCD1;1-GFP fusion protein is present in the nucleus. Furthermore, overexpression of the PsnCYCD1;1 gene in Arabidopsis can promote cell division and lead to small cell generation and cytokinin response, resulting in curved leaves and twisted inflorescence stems. Moreover, the transcriptional levels of endogenous genes, such as ASs, KNATs, EXP10, and PHB, were upregulated by PsnCYCD1;1. Together, our results indicated that PsnCYCD1;1 participates in cell division by cytokinin response, providing new information on controlling plant architecture in woody plants.

show abstract

“…In contrast, overexpression of CYCD3 in Arabidopsis caused morphological changes, such as delayed SAM and leaf senescence and leaf explant tissues, proliferated independent of cytokinin ( Riou-Khamlichi et al, 1999 ). Overexpression of PtoCYCD3;3 in poplar promoted the development of cambium and vascular bundle ( Guan et al, 2021 ). Targeted expression of AtCYCD7;1 to the central cells and early endosperm reduced the seed number in each silique and increased seed size ( Sornay et al, 2015 , 2016 ).…”

Section: Discussionmentioning

confidence: 99%

Ectopic Expression of Poplar PsnCYCD1;1 Reduces Cell Size and Regulates Flower Organ Development in Nicotiana tabacum

et al. 2022

View full text Add to dashboard Cite

The D-type cyclin (CYCD) gene, as the rate-limiting enzyme in the G1 phase of cell cycle, plays a vital role in the process of plant growth and development. Early studies on plant cyclin mostly focused on herbs, such as Arabidopsis thaliana. The sustainable growth ability of woody plants is a unique characteristic in the study of plant cyclin. Here, the promoter of PsnCYCD1;1 was cloned from poplar by PCR and genetically transformed into tobacco. A strong GUS activity was observed in the areas with vigorous cell division, such as stem tips, lateral buds, and young leaves. The PsnCYCD1;1-GFP fusion expression vector was transformed into tobacco, and the green fluorescence signal was observed in the nucleus. Compared with the control plant, the transgenic tobacco showed significant changes in the flower organs, such as enlargement of sepals, petals, and fruits. Furthermore, the stems of transgenic plants were slightly curved at each stem node, the leaves were curled on the adaxial side, and the fruits were seriously aborted after artificial pollination. Microscopic observation showed that the epidermal cells of petals, leaves, and seed coats of transgenic plants became smaller. The transcriptional levels of endogenous genes, such as NtCYCDs, NtSTM, NtKNAT1, and NtASs, were upregulated by PsnCYCD1;1. Therefore, PsnCYCD1;1 gene played an important role in the regulation of flower organ and stem development, providing new understanding for the functional characterization of CYCD gene and new resources for improving the ornamental value of horticultural plants.

show abstract

Overexpression of PtoCYCD3;3 Promotes Growth and Causes Leaf Wrinkle and Branch Appearance in Populus

Cited by 16 publications

References 45 publications

Induction and Characterization of Tetraploid Through Zygotic Chromosome Doubling in Eucalyptus urophylla

Induction and Characterization of Tetraploid Through Zygotic Chromosome Doubling in Eucalyptus urophylla

Overexpression Populus d-Type Cyclin Gene PsnCYCD1;1 Influences Cell Division and Produces Curved Leaf in Arabidopsis thaliana

Ectopic Expression of Poplar PsnCYCD1;1 Reduces Cell Size and Regulates Flower Organ Development in Nicotiana tabacum

Contact Info

Product

Resources

About