Overexpression of Phytochelatin Synthase in Arabidopsis Leads to Enhanced Arsenic Tolerance and Cadmium Hypersensitivity

Li, Yujing; Dhankher, Om Parkash; Carreira, Laura; Lee, David; Chen, Alice; Schroeder, Julian; Balish, Rebecca S.; Meagher, Richard B

doi:10.1093/pcp/pci034

Cited by 47 publications

(76 citation statements)

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“…Averaged COD concentration of infl uent in swine wastewaters was 1,160 mg/L (Lee et al 2004 ). The excellent COD removal by a subsurface fl ow constructed wetland was accomplished by a good cooperation between physical and microbial mechanisms, where the former mechanism made 52-74 % of contribution, and the latter 26-48 % (Lee et al 2004 ).…”

Section: Cod and Bod Removalmentioning

confidence: 98%

“…Both systems were signifi cantly effi cient at reducing nitrate (NO 3 -N), principally through a signifi cant reduction in soil surface runoff volume (50-57 %) together with a reduction in NO 3 -N concentration (7-13 %) to give an average reduction in total NO 3 -N load of 53-61 %. Lee et al ( 2004 ) studied the subsurface fl ow constructed wetlands performance at different high loading rates of swine effl uent. The wetland vegetated with water hyacinth exhibited higher removal rates than most other wetlands, although it gave low reduction effi ciencies.…”

Section: Nutrients Removalmentioning

confidence: 99%

“…On the other hand, Rizzo et al ( 2012 ) found more than 70 % of suspended matter removal in both treatments with and without plants for a feedlot effl uent. The suspended solids were removed entirely by physical processes, involving sedimentation, fi ltration, and adsorption in subsurface fl ow constructed wetland (Lee et al 2004 ).…”

Section: Solids Removalmentioning

confidence: 99%

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Phytoremediation

2015

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Section: Cod and Bod Removalmentioning

confidence: 98%

Section: Nutrients Removalmentioning

confidence: 99%

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Phytoremediation

2015

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“…Overexpressing of PCS in Arabidopsis led to contradictory success in heavy metal tolerance: the plants became As tolerant, but Cd hypersensitive (Li et al 2004). Due to the application of both heavy metals total thiol concentration increased, but the spectrum of thiols was very different.…”

Section: Glumentioning

confidence: 99%

“…Additionally, plants can be genetically engineered to remove and/or degrade organic pollutants (Dietz and Schnoor 2001;Hannink et al 2002). However, genetic transformation events will become negative for phytoremediation purposes if they result in unchanged or even lower shoot concentration of metals (Pilon-Smits et al 2000;Harada et al 2001;Li et al 2004;Krämer 2005) or lower growth also under polluted soils.…”

Section: Molecular Engineering To Improve the Performance Of Plants Imentioning

confidence: 99%

Heavy Metal Resistance and Phytoremediation with Transgenic Trees

Peuke

Rennenberg

2006

Tree Transgenesis

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Enhanced arsenic accumulation by engineered yeast cells expressing Arabidopsis thaliana phytochelatin synthase

Singh

Lee

DaSilva

et al. 2007

Biotech & Bioengineering

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Phytochelatins (PCs) are naturally occurring peptides with high-binding capabilities for a wide range of heavy metals including arsenic (As). PCs are enzymatically synthesized by phytochelatin synthases and contain a (g-Glu-Cys) n moiety terminated by a Gly residue that makes them relatively proteolysis resistant. In this study, PCs were introduced by expressing Arabidopsis thaliana Phytochelatin Synthase (AtPCS) in the yeast Saccharomyces cerevisiae for enhanced As accumulation and removal. PCs production in yeast resulted in six times higher As accumulation as compared to the control strain under a wide range of As concentrations. For the high-arsenic concentration, PCs production led to a substantial decrease in levels of PC precursors such as glutathione (GSH) and g-glutamyl cysteine (g-EC). The levels of As(III) accumulation were found to be similar between AtPCS-expressing wild type strain and AtPCS-expressing acr3D strain lacking the arsenic efflux system, suggesting that the arsenic uptake may become limiting. This is further supported by the roughly 1:3 stoichiometric ratio between arsenic and PC2 (n ¼ 2) level (comparing with a theoretical value of 1:2), indicating an excess availability of PCs inside the cells. However, at lower As(III) concentration, PC production became limiting and an additive effect on arsenic accumulation was observed for strain lacking the efflux system. More importantly, even resting cells expressing AtPCS pre-cultured in Zn 2þ enriched media showed PCs production and two times higher arsenic removal than the control strain. These results open up the possibility of using cells expressing AtPCS as an inexpensive sorbent for the removal of toxic arsenic.

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Overexpression of Phytochelatin Synthase in Arabidopsis Leads to Enhanced Arsenic Tolerance and Cadmium Hypersensitivity

Cited by 47 publications

References 0 publications

Phytoremediation

Phytoremediation

Heavy Metal Resistance and Phytoremediation with Transgenic Trees

Enhanced arsenic accumulation by engineered yeast cells expressing Arabidopsis thaliana phytochelatin synthase

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