1993
DOI: 10.1006/bbrc.1993.2229
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Overexpression of p59-HBI (FKBP59), Full Length and Domains, and Characterization of PPlase Activity

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Cited by 73 publications
(73 citation statements)
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“…The FK1 domain is responsible for the PPIase activity of FKBP52, whereas the FK2 domain shows no PPIase activity (27,28). Detailed structural information for FK1 has been reported (17).…”
Section: Methodsmentioning
confidence: 99%
“…The FK1 domain is responsible for the PPIase activity of FKBP52, whereas the FK2 domain shows no PPIase activity (27,28). Detailed structural information for FK1 has been reported (17).…”
Section: Methodsmentioning
confidence: 99%
“…Overexpression and purification of the entire protein and of various combinations of the domains confirmed that the immunosuppressant binding site and rotamase activity of FKEiP59 are localized in the N-terminal domain (Chambraud et al, 1993). In contrast with the high similarity in the primary sequence (especially the conservation of the ligand-binding residues) and PProIase activity with human FKBP12, the binding affinity of FK506 or rapamycin is decreased by about two orders of magnitude (Renoir et al, 1994).…”
mentioning
confidence: 93%
“…The N-terminal domain of FKBP59 was inserted into the vector pGEX 11T (Pharmacia) and overexpressed in E. coli as already described (Chambraud et al, 1993). An Asp-Ile sequence was inserted at the N-terminal and Val-Pro at the C-terminal of the original sequence as recognition sites for EcoRV and HpaI, respectively.…”
Section: Methodsmentioning
confidence: 99%
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“…FKBP52 displays a modular organization (11)(12)(13) and only the NH 2 -terminal part of the protein is responsible for the immunophilin character of the whole protein; it binds FK506 and rapamycin and displays a peptidylprolyl isomerase function characteristic of the immunophilin family (14). Although this NH 2 -terminal part shares high structural and functional homologies with FKBP12, it does not inhibit the phosphatase activity of calcineurin, in contrast with FKBP12 (15)(16)(17).…”
mentioning
confidence: 99%