Overexpression of p53 protein and its significance for recurrent progressive bladder tumours

Abstract

Objective To determine the prognostic value of the over-was not linked to recurrence (P=0.5) but was related to worsening histological stage (P<0.01) and increas-expression of p53 protein as determined by immunohistochemistry in recurrent progressive transitional ing grade (P<0.01). Regression analysis showed that overexpression of p53 for the primary tumour was cell carcinomas of the bladder. Patients and methods A total of 222 tumours from 86 not of predictive prognostic value for death from bladder cancer, time to progression or time to recur-patients with recurrent disease, 20 from patients with no evidence of recurrence after resection of initial rence. Tumour grade was the only variable of prognostic value in all the statistical models. Patients with tumour and 11 normal bladder (controls) were investigated. Using a microwave technique to expose overexpression of p53 showed no reduction in overall survival. antigens, formalin-fixed sections were immunohistochemically stained for p53 using a polyclonal anti-Conclusion These findings suggest that overexpression of p53, as determined immunohistochemically, serum. Two independent observers scored the sections for evidence of overexpression of p53.appears to have no predictive prognostic value over stage and grade in bladder tumours. Results Of 86 patients with recurrent disease, 51 demonstrated overexpression of p53 protein, as did six of 20 Keywords p53 overexpression, bladder cancer, p53 immunohistochemistry, prognosis patients with non-recurrent disease. Overexpression lates and switches off replication to allow time for extra

Immunohistochemical determination of p53 overexpression

“…Possibly the high percentage of p53 positivity in carcinoma in situ is more suggestive of a different molecular pathway leading to cancerogenesis. This agrees with the theory that carcinoma in situ turnout cells are so undifferentiated that they cannot grow invasively, p53 overexpression has been shown to correlate significantly with grade and stage, which suggests that p53 inactivation might be an early event in bladder cancer [24]. In the present study the association with grade and stage and the high percentage of p53 overexpression in Ta tumours further implicate that p53 inactivation may occur early in the evolution of bladder cancer.…”

Prognostic Significance of p53 Protein Overexpression in Transitional Cell Carcinoma of the Renal Pelvis and Ureter

“…These reports have also demonstrated p53 protein overexpression in 28.1-54% [23][24][25] of invasive tumors. In other early studies of bladder cancer, the incidence of p53 protein overexpression in invasive tumors ranged from 34 to 54.5% [17,20,21]. Based on the results of these studies, we speculate that p53 protein overexpression of upper urothelial cancer may not be higher than that of bladder cancer.…”

“…There have been many studies of p53 expression in transitional urothelial tumors of the bladder [12,[16][17][18][19][20][21][22]. These reports suggest that mutations of the p53 gene and/ or overexpression of p53 protein correlate with recognized prognostic features including histologic grade and pathologic stage [12, 16-18, 20, 21].…”

“…Nuclear accumulation of p53 protein detected by immunohistochemistry closely correlates with the presence of mutations of the p53 gene [12][13][14][15]. There have been many reports describing the relationships between histopathologic features, prognosis, and mutations of the p53 gene or nuclear accumulation of p53 protein in more common carcinomas of the bladder [12,[16][17][18][19][20][21][22]. However, few studies have evaluated the significance of the nuclear accumulation of p53 protein or mutations of the p53 gene in urothelial tumors of the renal pelvis and ureter [23][24][25].…”

Die Her2/neu-Expression beim lokal fortgeschrittenen Harnblasenkarzinom: Ansatz für eine molekulare Therapie?

“…demonstriert werden konnte[10]. Die Korrelation mit dem Auftreten von Tumorrezidiven, die wir in unserer Studie nicht zeigen konnten, wurde auch durch andere Arbeitsgruppen nur teilweise bestätigt[29].Bei der Überlebensanalyse unserer Daten ergab sich kein Zusam− menhang zwischen dem Her2/neu−Status und dem Gesamt− oder rezidivfreien Überleben. Auch dieses Ergebnis wird in der Literatur in unterschiedlicher Weise berichtet: Während Tzai u. Mitarb.…”