Overexpression of OsNINJA1 negatively affects a part of OsMYC2-mediated abiotic and biotic responses in rice

Kashihara, Keita; Onohata, Tomonori; Okamoto, Yuki; Uji, Yuya; Mochizuki, Susumu; Akimitsu, Kazuya; Gomi, Kenji

doi:10.1016/j.jplph.2018.11.009

Cited by 16 publications

(18 citation statements)

References 71 publications

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“…Plant and bacteria growth conditions were as previously described, they are grown and cultured in our laboratory by ourselves [15]. The fully opened fifth leaf blades of rice plants were inoculated using the clipping inoculation technique [57].…”

Section: Methodsmentioning

confidence: 99%

“…Total RNA was extracted from rice leaf blades from plants of different genotypes and treatments using Trizol (Thermo Fisher Scientific, Waltham, MA, USA) according to the manufacturer’s instructions. RT-qPCR was performed as previously described [15,59]. The sequences of the gene-specific primers used in this study are presented in the Supplementary Table S1.…”

Section: Methodsmentioning

confidence: 99%

“…An analysis of protein–protein interactions using a yeast two-hybrid system was performed as previously described [15]. Images of yeast cells were obtained at day 3.…”

Section: Methodsmentioning

confidence: 99%

“…The basic helix–loop–helix (bHLH)-type TF OsMYC2, which is the rice homologue of AtMYC2, positively regulates the JA-mediated defense response against Xoo in rice [13]. OsNINJA1, which is the rice homologue of Arabidopsis NOVEL INTERACTOR OF JAZ (AtNINJA) [14], acts as a negative regulator of the OsMYC2-mediated defense response against Xoo in rice [15]. JA-induced volatiles such as monoterpenes and sesquiterpenes act as antibacterial or signaling compounds in the defense response against Xoo [16,17,18,19,20].…”

Section: Introductionmentioning

confidence: 99%

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Jasmonic Acid-Induced VQ-Motif-Containing Protein OsVQ13 Influences the OsWRKY45 Signaling Pathway and Grain Size by Associating with OsMPK6 in Rice

Uji

Kashihara

Kiyama

et al. 2019

IJMS

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Jasmonic acid (JA) is a plant hormone that plays an important role in the defense response and stable growth of rice. In this study, we investigated the role of the JA-responsive valine-glutamine (VQ)-motif-containing protein OsVQ13 in JA signaling in rice. OsVQ13 was primarily located in the nucleus and cytoplasm. The transgenic rice plants overexpressing OsVQ13 exhibited a JA-hypersensitive phenotype and increased JA-induced resistance to Xanthomonas oryzae pv. oryzae (Xoo), which is the bacteria that causes rice bacterial blight, one of the most serious diseases in rice. Furthermore, we identified a mitogen-activated protein kinase, OsMPK6, as an OsVQ13-associating protein. The expression of genes regulated by OsWRKY45, an important WRKY-type transcription factor for Xoo resistance that is known to be regulated by OsMPK6, was upregulated in OsVQ13-overexpressing rice plants. The grain size of OsVQ13-overexpressing rice plants was also larger than that of the wild type. These results indicated that OsVQ13 positively regulated JA signaling by activating the OsMPK6–OsWRKY45 signaling pathway in rice.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

“…An analysis of protein–protein interactions using a yeast two-hybrid system was performed as previously described [15]. Images of yeast cells were obtained at day 3.…”

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Jasmonic Acid-Induced VQ-Motif-Containing Protein OsVQ13 Influences the OsWRKY45 Signaling Pathway and Grain Size by Associating with OsMPK6 in Rice

Uji

Kashihara

Kiyama

et al. 2019

IJMS

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“…Among the many possible candidates, three functional hypothesis were retained. First, the increase of susceptibility could be due to the presence of regulators of biotic stress responses like RACK1B [58], OsNINJA1 [59,60] and OsSYP71 [61]. Second, the phenotype could arise from antagonistic pleiotropy in a gene such as OsMATE2 [61,62], that regulates plant growth in response to nitrogen while negatively affecting disease resistance.…”

Section: Nis2 a Qtl Associated With Increased Susceptibility Under Hmentioning

confidence: 99%

Genome-Wide Association of Rice Response to Blast Fungus Identifies Loci for Robust Resistance Under High Nitrogen.

Frontini

Boisnard²,

Frouin

et al. 2020

Preprint

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Background: Nitrogen fertilization is known to increase disease susceptibility, a phenomenon called Nitrogen-Induced Susceptibility (NIS). In rice, this phenomenon has been observed in infections with the blast fungus Magnaporthe oryzae. A previous classical genetic study revealed a locus (NIS1) that enhances susceptibility to rice blast under high nitrogen fertilization. In order to further address the underlying genetics of plasticity in susceptibility to rice blast after fertilization, we analyzed NIS under greenhouse-controlled conditions in a panel of 139 temperate japonica rice strains. A genome-wide association analysis was conducted to identify loci potentially involved in NIS by comparing susceptibility loci identified under high and low nitrogen conditions, an approach allowing for the identification of loci validated across different nitrogen environments. We also used a novel NIS Index to identify loci potentially contributing to plasticity in susceptibility under different nitrogen fertilization regimes.Results: A global NIS effect was observed in the population, with the density of lesions increasing by 8%, on average, under high nitrogen fertilization. Three new QTL, other than NIS1, were identified. A rare allele of the RRobN1 locus on chromosome 6 provides robust resistance in high and low nitrogen environments. A frequent allele of the NIS2 locus, on chromosome 5, exacerbates blast susceptibility under the high nitrogen condition. Finally, an allele of NIS3, on chromosome 10, buffers the increase of susceptibility arising from nitrogen fertilization but increases global levels of susceptibility. This allele is almost fixed in temperate japonicas, as a probable consequence of genetic hitchhiking with a locus involved in cold stress adaptation.Conclusions: Our results extend to an entire rice subspecies the initial finding that nitrogen increases rice blast susceptibility. We demonstrate the usefulness of estimating plasticity for the identification of novel loci involved in the response of rice to the blast fungus under different nitrogen regimes.

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Targeting Phytohormone Levels for Genetic Engineering Abiotic Stress Tolerance in Rice

Hossain

Alam

Jahan

et al. 2020

Rice Research for Quality Improvement: Genomics and Genetic Engineering

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Overexpression of OsNINJA1 negatively affects a part of OsMYC2-mediated abiotic and biotic responses in rice

Cited by 16 publications

References 71 publications

Jasmonic Acid-Induced VQ-Motif-Containing Protein OsVQ13 Influences the OsWRKY45 Signaling Pathway and Grain Size by Associating with OsMPK6 in Rice

Jasmonic Acid-Induced VQ-Motif-Containing Protein OsVQ13 Influences the OsWRKY45 Signaling Pathway and Grain Size by Associating with OsMPK6 in Rice

Genome-Wide Association of Rice Response to Blast Fungus Identifies Loci for Robust Resistance Under High Nitrogen.

Targeting Phytohormone Levels for Genetic Engineering Abiotic Stress Tolerance in Rice

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