Overexpression of OsIAAGLU reveals a role for IAA–glucose conjugation in modulating rice plant architecture

Yu, Xiaolu; Wang, Haiyan; Leung, David W. M.; He, Zhi-dan; Zhang, Jianjun; Peng, Xinxiang; Liu, Ee

doi:10.1007/s00299-019-02402-4

Cited by 25 publications

(24 citation statements)

References 25 publications

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“…Possibly due to reduced auxin response, OsIAGT1 overexpression lines showed reduced shoot height and root length. As a complement to the recent report on the same UGT protein OsIAAGLU (Yu et al 2019), our results confirmed that OsIAGT1 /OsIAAGLU is capable of catalyzing auxin glucosylation in rice cells, and further strengthened the role of OsIAGT1/OsIAAGLU in mediating auxin homeostasis and thus regulating auxin-related rice growth.…”

Section: Discussionsupporting

confidence: 88%

“…Moreover, we found that expression of OsIAGT1 is auxin-induced, and ectopic expression of OsIAGT1 leads to declined endogenous IAA content, as well as reduced expression of auxin-responsive genes, which caused the reduced plant height and root length in OsIAGT1 overexpression lines. OsIAGT1 is the same protein to a recently reported UGT in another study, which was designated as OsIAAGLU (Yu et al 2019). Our results further strengthen the role of OsIAGT1/OsIAAGLU in mediating auxin homeostasis by catalyzing auxin glucosylation and in regulating rice growth and development.…”

Section: Introductionmentioning

confidence: 73%

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OsIAGT1 Is a Glucosyltransferase Gene Involved in the Glucose Conjugation of Auxins in Rice

Lei

Chen

Xiao

et al. 2019

Rice

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BackgroundIn cereal crop rice, auxin is known as an important class of plant hormone that regulates a plethora of plant growth and development. Glycosylation of auxin is known to be one of the important mechanisms mediating auxin homeostasis. However, the relevant auxin glucosyltransferase (GT) in rice still remains largely unknown.ResultsIn this study, using known auxin glucosyltransferases from other species as queries, twelve putative auxin UDP-glycosyltransferase (UGT) genes were cloned from rice and the one showing highest sequence similarity, named as OsIAGT1, was expressed as recombinant protein. In vitro enzymatic analysis showed that recombinant OsIAGT1 was capable of catalyzing glucosylation of IAA, IBA and other auxin analogs, and that OsIAGT1 is quite tolerant to a broad range of reaction conditions with peak activity at 30 °С and pH 8.0. OsIAGT1 showed favorite activity towards native auxins over artificially synthesized ones. Further study indicated that expression of OsIAGT1 can be upregulated by auxin in rice, and with OsIAGT1 overexpressing lines we confirmed that OsIAGT1 is indeed able to glucosylate IAA in vivo. Consistently, ectopic expression of OsIAGT1 leads to declined endogenous IAA content, as well as upregulated auxin synthesis genes and reduced expression of auxin-responsive genes, which likely leads to the reduced plant stature and root length in OsIAGT1 overexpression lines.ConclusionOur result indicated that OsIAGT1 plays an important role in mediating auxin homeostasis by catalyzing auxin glucosylation, and by which OsIAGT1 regulates growth and development in rice.

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Section: Discussionsupporting

confidence: 88%

Section: Introductionmentioning

confidence: 73%

OsIAGT1 Is a Glucosyltransferase Gene Involved in the Glucose Conjugation of Auxins in Rice

Lei

Chen

Xiao

et al. 2019

Rice

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“…6). Several genetic and molecular biology studies have indicated that UDPglucosyltransferase family genes are involved in the regulation of plant architecture [91][92][93] .…”

Section: Discussionmentioning

confidence: 99%

Bulked Segregant Rna Sequencing (Bsr-Seq) Identifies a Novel Allele Associated With Weeping Traits in Prunus Mume

Zhuo¹,

Zheng²,

Zhang³

et al. 2021

Front. Agr. Sci. Eng.

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“…OsIAGLU showed 67% amino acid identity with ZmIAGLU, which possessed the same function to glucosylate the hydroxyl of IAA (Yu et al, 2019). Similarly, BnUGT1 and AtUGT72B1 shared 85% sequence identity and the same O-glycosylation function toward 3,4-dichlorophenol; differentially, only AtUGT72B1 could glucosylate the N-position of 3,4dichloraaniline (Brazier-Hicks et al, 2007).…”

Section: Specificity Of N-glucosylationmentioning

confidence: 96%

“…Until Ljung et al (2001) detected IAA-N-glucoside and IAA-Asp-Nglucoside in Scots pine (Pinus sylvestris), researchers started to notice the new metabolic branch for IAA (Ljung et al, 2001). However, no N-glucosyltransferase (NGT) gene was found up to now, even though another two new IAGlc synthases, OsIAGT1 and OsIAGLU were recently reported to produce IAA-O-glucoside in rice (Oryza sativa) (Liu et al, 2019;Yu et al, 2019).…”

Section: Introductionmentioning

confidence: 99%

N-glucosyltransferase GbNGT1 from Ginkgo complement auxin metabolic pathway

Yin

Zhang

Wang

et al. 2020

Preprint

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As a group of the most important phytohormone, auxin homeostasis is regulated in a complex manner. Generally, auxin conjugations especially IAA glucosides are dominant on high auxin level conditions. Former terminal glucosylation researches mainly focus on O-position, while IAA-N-glucoside or IAA-Asp-N-glucoside has been neglected since their found in 2001. In our study, IAA-Asp-N-glucoside was firstly found specifically abundant (as high as 4.13 mg/g) in ginkgo seeds of 58 cultivars from Ginkgo Resource Nursery built in 1990. Furthermore, a novel N-glucosyltransferase GbNGT1, which could catalyze IAA-Asp and IAA to form their corresponding N-glucoside, was identified through differential transcriptome analysis and in vitro enzymatic test. The enzyme was demonstrated to possess specific catalyze capacity toward the N-position of IAA-amino acid or IAA among 52 substrates, and was typical of acid tolerance, metal ion independence and high temperature sensitivity. Docking and site-directed mutagenesis of this enzyme confirmed that E15G mutant could almost abolish enzyme catalytic activity towards IAA-Asp and IAA in vitro and in vivo. The IAA modification of GbNGT1 and GbGH3.5 was verified by transient expression assay in Nicotiana benthamiana. In conclusion, our results complement the terminal metabolic pathway of auxin, and the specific catalytic function of GbNGT1 towards IAA-amino acid provide a new way to biosynthesis indole-amide compounds.HighlightThe N-glucosylation of IAA or IAA-amino acids in auxin metabolism had been neglected over decades, our work for GbNGT1 redeems the missing chain of auxin metabolic pathway.

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Overexpression of OsIAAGLU reveals a role for IAA–glucose conjugation in modulating rice plant architecture

Cited by 25 publications

References 25 publications

OsIAGT1 Is a Glucosyltransferase Gene Involved in the Glucose Conjugation of Auxins in Rice

OsIAGT1 Is a Glucosyltransferase Gene Involved in the Glucose Conjugation of Auxins in Rice

Bulked Segregant Rna Sequencing (Bsr-Seq) Identifies a Novel Allele Associated With Weeping Traits in Prunus Mume

N-glucosyltransferase GbNGT1 from Ginkgo complement auxin metabolic pathway

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