Overexpression of OsEXPA8, a Root-Specific Gene, Improves Rice Growth and Root System Architecture by Facilitating Cell Extension

Ma, Nana; Wang, Ying; Qiu, Shichun; Kang, Zhenhui; Che, Shugang; Wang, Guixue; Huang, Junli

doi:10.1371/journal.pone.0075997

Cited by 100 publications

(75 citation statements)

References 46 publications

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“…The mutant phenotype proved to be partially reversible by constitutively expressing OsEXPA30, which is a gene encoding an RH-specific expansin. A recent report showed that the overexpression of OsEXPA8 increases the length of rice RHs, primary roots, and the number of lateral roots that are formed (Ma et al, 2013).…”

Section: "Tis Within Ourselves That We Are Thus or Thus": Elongation mentioning

confidence: 99%

Root Hair Development in the Grasses: What We Already Know and What We Still Need to Know

2015

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ORCID IDs: 0000-0002-5261-6662 (M.Ma.); 0000-0001-7390-7234 (I.S.).A priority in many crop improvement programs for a long time has been to enhance the tolerance level of plants to both abiotic and biotic stress. Recognition that the root system is the prime determinant of a plant's ability to extract both water and minerals from the soil implies that its architecture is an important variable underlying a cultivar's adaptation. The density and/or length of the root hairs (RHs) that are formed are thought to have a major bearing on the plant's performance under stressful conditions. Any attempt to improve a crop's root system will require a detailed understanding of the processes of RH differentiation. Recent progress in uncovering the molecular basis of root epidermis specialization has been recorded in the grasses. This review seeks to present the current view of RH differentiation in grass species. It combines what has been learned from molecular-based analyses, histological studies, and observation of the phenotypes of both laboratory-and field-grown plants.

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Section: "Tis Within Ourselves That We Are Thus or Thus": Elongation mentioning

confidence: 99%

Root Hair Development in the Grasses: What We Already Know and What We Still Need to Know

2015

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“…For instance, CSLF6, a putative cellulose-like synthase family member, is required for rice primary and adventitious root development through a phosphate accumulation-dependent pathway (Jin et al, 2015). Rice expansin genes, including OsEXPA8, OsEXPA17, and OsEXPB5, are reported to modulate root development (Won et al, 2010;ZhiMing et al, 2011;Ma et al, 2013). In addition, CESAs (cellulose synthases) are delivered to cell plate and deposit cellulose via phragmoplast-associated vesicles during cell division in Arabidopsis root (Miart et al, 2014).…”

Section: Wox11 Recruits Ada2/gcn5 To Establish Gene Expression Programentioning

confidence: 99%

Rice Homeodomain Protein WOX11 Recruits a Histone Acetyltransferase Complex to Establish Programs of Cell Proliferation of Crown Root Meristem

et al. 2017

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Shoot-borne crown roots are the major root system in cereals. Previous work has shown that the Wuschel-related homeobox gene WOX11 is necessary and sufficient to promote rice (Oryza sativa) crown root emergence and elongation. Here, we show that WOX11 recruits the ADA2-GCN5 histone acetyltransferase module to activate downstream target genes in crown root meristem. Rice ADA2 and GCN5 genes are highly expressed in root meristem and are shown to be essential for cell division and growth. WOX11 and ADA2-GCN5 commonly target and regulate a set of root-specific genes involved in energy metabolism, cell wall biosynthesis, and hormone response, some of which are known to be important for root development. The results indicate that the recruitment of ADA2-GCN5 by WOX11 establishes gene expression programs of crown root meristem cell division and suggest that permissive chromatin modification involving histone acetylation is a strategy for WOX11 to stimulate root meristem development.

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“…Notably, one gene (OsEXPA8, LOC_Os01g14650.1) showed twofold higher expression in the tips compared with the bases under hypoxia and anoxia, whereas no difference was seen under normoxia (Table S1). When this gene was over-expressed in rice, improved root growth by enabling cell elongation was observed (Ma et al, 2013). The correlation between expansins and rapid elongation in rice internodes has also been demonstrated in deep-water rice (Cho and Kende, 1997a,b;Lee et al, 2001), in which submerged internodes were enriched in expansins compared with non-elongating internodes.…”

Section: Expression Of Growth-related Genes Based On Gradients In Anamentioning

confidence: 83%

Mechanisms of growth and patterns of gene expression in oxygen‐deprived rice coleoptiles

et al. 2015

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These authors contributed equally to this work. SUMMARYColeoptiles of rice (Oryza sativa) seedlings grown under water commonly elongate by up to 1 mm h À1 to reach the atmosphere. We initially analysed this highly specialized phenomenon by measuring epidermal cell lengths along the coleoptile axis to determine elongation rates. This revealed a cohort of cells in the basal zone that elongated rapidly following emergence from the embryo, reaching 200 lm within 12 h. After filming coleoptiles in vivo for a day, kinematic analysis was applied. Eight time-sliced 'segments' were defined by their emergence from the embryo at four-hourly intervals, revealing a mathematically simple growth model. Each segment entering the coleoptile from the embryo elongated at a constant velocity, resulting in accelerating growth for the entire organ. Consistent with the epidermal cell lengths, relative rates of elongation (mm mm À1 h À1 ) were tenfold greater in the small, newly emerged basal segments than the older distal tip segments. This steep axial gradient defined two contrasting growth zones (bases versus tips) in which we measured ATP production and protein, RNA and DNA content, and analysed the global transcriptome under steady-state normoxia, hypoxia (3% O 2 ) and anoxia. Determination of the transcriptome revealed tip-specific induction of genes encoding TCP [Teosinte Branched1 (Tb1) of maize, Cycloidea (Cyc), and Proliferating Cell Factor (Pcf)] transcription factors, RNA helicases, ribosomal proteins and proteins involved in protein folding, whilst expression of F-box domain-containing proteins in the ubiquitin E3-SCF complex (Skp, Cullin, F-box containing complex) was induced specifically in bases under low oxygen conditions. We ascribed the sustained elongation under hypoxia to hypoxia-specific responses such as controlled suppression of photosystem components and induction of RNA binding/splicing functions, indicating preferential allocation of energy to cell extension.

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Overexpression of OsEXPA8, a Root-Specific Gene, Improves Rice Growth and Root System Architecture by Facilitating Cell Extension

Cited by 100 publications

References 46 publications

Root Hair Development in the Grasses: What We Already Know and What We Still Need to Know

Root Hair Development in the Grasses: What We Already Know and What We Still Need to Know

Rice Homeodomain Protein WOX11 Recruits a Histone Acetyltransferase Complex to Establish Programs of Cell Proliferation of Crown Root Meristem

Mechanisms of growth and patterns of gene expression in oxygen‐deprived rice coleoptiles

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