Overexpression of NtSOS2 From Halophyte Plant N. tangutorum Enhances Tolerance to Salt Stress in Arabidopsis

Zhu, Liming; Li, Mengjuan; Huo, Junnan; Lian, Ziming; Liu, Yuxin; Lu, Lu; Lu, Ye; Hao, Zhaodong; Shi, Jisen; Cheng, Tielong; Chen, Jinhui

doi:10.3389/fpls.2021.716855

Cited by 6 publications

(3 citation statements)

References 54 publications

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“…It has been reported that in addition to being involved in the response to salt stress, the SOS2 gene can also be induced by abscisic acid (ABA) and various abiotic stresses and may have a variety of physiological functions [24]. So far, the SOS2 gene has been functionally characterized in a variety of plant species, and it has been shown that overexpression of SOS2 can improve the salt tolerance of different transgenic plants [25][26][27]. Unfortunately, research on the function of the SOS2 gene in mulberry is still lacking.…”

Section: Introductionmentioning

confidence: 99%

Unraveling the Contribution of MulSOS2 in Conferring Salinity Tolerance in Mulberry (Morus atropurpurea Roxb)

Wang,

Han,

Wang

et al. 2024

IJMS

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Salinity is one of the most serious threats to sustainable agriculture. The Salt Overly Sensitive (SOS) signaling pathway plays an important role in salinity tolerance in plants, and the SOS2 gene plays a critical role in this pathway. Mulberry not only has important economic value but also is an important ecological tree species; however, the roles of the SOS2 gene associated with salt stress have not been reported in mulberry. To gain insight into the response of mulberry to salt stress, SOS2 (designated MulSOS2) was cloned from mulberry (Morus atropurpurea Roxb), and sequence analysis of the amino acids of MulSOS2 showed that it shares some conserved domains with its homologs from other plant species. Our data showed that the MulSOS2 gene was expressed at different levels in different tissues of mulberry, and its expression was induced substantially not only by NaCl but also by ABA. In addition, MulSOS2 was exogenously expressed in Arabidopsis, and the results showed that under salt stress, transgenic MulSOS2 plants accumulated more proline and less malondialdehyde than the wild-type plants and exhibited increased tolerance to salt stress. Moreover, the MulSOS2 gene was transiently overexpressed in mulberry leaves and stably overexpressed in the hairy roots, and similar results were obtained for resistance to salt stress in transgenic mulberry plants. Taken together, the results of this study are helpful to further explore the function of the MulSOS2 gene, which provides a valuable gene for the genetic breeding of salt tolerance in mulberry.

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Section: Introductionmentioning

confidence: 99%

Unraveling the Contribution of MulSOS2 in Conferring Salinity Tolerance in Mulberry (Morus atropurpurea Roxb)

Wang,

Han,

Wang

et al. 2024

IJMS

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“…SOS2 exists as a form of protein kinase in the SOS signaling pathway, which in turn activates SOS1 to bring about sodium ion homeostasis and salt tolerance (Ali et al, 2021). SOS3, which encodes an EF-handed Ca 2+ binding protein, can sense calcium signals elicited by salt stress, interact with SOS2, and activate SOS2 (Zhu et al, 2021).…”

Section: Introductionmentioning

confidence: 99%

Genome-wide characterization of SOS1 gene family in potato (Solanum tuberosum) and expression analyses under salt and hormone stress

et al. 2023

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Salt Overly Sensitive 1 (SOS1) is one of the members of the Salt Overly Sensitive (SOS) signaling pathway and plays critical salt tolerance determinant in plants, while the characterization of the SOS1 family in potato (Solanum tuberosum) is lacking. In this study, 37 StSOS1s were identified and found to be unevenly distributed across 10 chromosomes, with most of them located on the plasma membrane. Promoter analysis revealed that the majority of these StSOS1 genes contain abundant cis-elements involved in various abiotic stress responses. Tissue specific expression showed that 21 of the 37 StSOS1s were widely expressed in various tissues or organs of the potato. Molecular interaction network analysis suggests that 25 StSOS1s may interact with other proteins involved in potassium ion transmembrane transport, response to salt stress, and cellular processes. In addition, collinearity analysis showed that 17, 8, 1 and 5 of orthologous StSOS1 genes were paired with those in tomato, pepper, tobacco, and Arabidopsis, respectively. Furthermore, RT-qPCR results revealed that the expression of StSOS1s were significant modulated by various abiotic stresses, in particular salt and abscisic acid stress. Furthermore, subcellular localization in Nicotiana benthamiana suggested that StSOS1-13 was located on the plasma membrane. These results extend the comprehensive overview of the StSOS1 gene family and set the stage for further analysis of the function of genes in SOS and hormone signaling pathways.

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“…The overexpression of the SOS2 gene improved salinity tolerance in different transgenic plants. In a recent study, Zhu et al (2021) reported that the overexpression of SOS2 from the halophytic plant Nitraria tangutorum enhanced salinity tolerance in Arabidopsis. However, functional genomics studies for assessing the effect of overexpression and knockdown of OsSOS2 on the agronomic features of rice under salinity and drought are not available.…”

Section: Introductionmentioning

confidence: 99%

Unraveling the contribution of OsSOS2 in conferring salinity and drought tolerance in a high‐yielding rice

Kumar

Basu

Singla‐Pareek

et al. 2022

Physiologia Plantarum

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Abiotic stresses are emerging as a potential threat to sustainable agriculture worldwide. Soil salinity and drought will be the major limiting factors for rice productivity in years to come. The Salt Overly Sensitive (SOS) pathway plays a key role in salinity tolerance by maintaining the cellular ion homeostasis, with SOS2, a S/T kinase, being a vital component. The present study investigated the role of the OsSOS2, a SOS2 homolog from rice, in improving salinity and drought tolerance. Transgenic plants with either overexpression (OE) or knockdown (KD) of OsSOS2 were raised in one of the high‐yielding cultivars of rice—IR64. Using a combined approach based on physiological, biochemical, anatomical, microscopic, molecular, and agronomic assessment, the evidence presented in this study advocates the role of OsSOS2 in improving salinity and drought tolerance in rice. The OE plants were found to have favorable ion and redox homeostasis when grown in the presence of salinity, while the KD plants showed the reverse pattern. Several key stress‐responsive genes were found to work in an orchestrated manner to contribute to this phenotype. Notably, the OE plants showed tolerance to stress at both the seedling and the reproductive stages, addressing the two most sensitive stages of the plant. Keeping in mind the importance of developing crops plants with tolerance to multiple stresses, the present study established the potential of OsSOS2 for biotechnological applications to improve salinity and drought stress tolerance in diverse cultivars of rice.

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Overexpression of NtSOS2 From Halophyte Plant N. tangutorum Enhances Tolerance to Salt Stress in Arabidopsis

Cited by 6 publications

References 54 publications

Unraveling the Contribution of MulSOS2 in Conferring Salinity Tolerance in Mulberry (Morus atropurpurea Roxb)

Unraveling the Contribution of MulSOS2 in Conferring Salinity Tolerance in Mulberry (Morus atropurpurea Roxb)

Genome-wide characterization of SOS1 gene family in potato (Solanum tuberosum) and expression analyses under salt and hormone stress

Unraveling the contribution of OsSOS2 in conferring salinity and drought tolerance in a high‐yielding rice

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