Overexpression of neuritin in gastric cancer

Abstract

Abstract. The aim of the present study was to investigate the expression of neuritin in gastric cancer tissues, in order to explore the association between the expression of neuritin and the occurrence and development of gastric cancer. Tissue specimens were collected from 58 patients with gastric cancer. Immunohistochemistry, western blot analysis and reverse transcription-polymerase chain reaction (RT-PCR) were used to determine the expression of neuritin in the gastric cancer and corresponding adjacent normal gastric tissues. The expression rate of neuritin in gastric cancer tissues was 96.55% (56/58), demonstrating no statistically significant difference from the expression rate in the adjacent normal tissues (94.83%) (P>0.05). However, the rate of strong neuritin expression in gastric cancer tissues (82.76%) was significantly increased compared with the rate in the adjacent normal tissues (15.52%) (P<0.05). Neuritin expression exhibited no correlation with the gender or age of patients, tumor-node-metastasis staging, tumor depth, presence of lymph node metastasis, histological or pathological type of the tumor or presence of distant metastasis (P>0.05). As determined by RT-PCR and western blot analysis, the mRNA expression of neuritin in gastric cancer tissues was markedly increased compared with the expression in the adjacent normal tissues. In conclusion, neuritin is highly expressed in gastric cancer tissues, suggesting that neuritin may act as a novel potential target for the treatment of gastric cancer. IntroductionNeuritin, also termed CPG15, is one of the candidate plasticity-associated genes that were first screened and identified from the rat hippocampus cDNA library by Nedivi et al in 1993 (1). This gene was found to promote the growth of neural processes, thus it was termed neuritin (1). Subsequent functional studies have verified the effects of neuritin on the growth of neural processes (2-4). In previous years, numerous studies have also revealed that, in addition to the nervous system, neuritin is also expressed in the liver, heart, lungs, skeletal muscle and other normal organs (5-7). Neuritin is also highly expressed in certain tumor tissues, including Kaposi's sarcoma (5-9), which indicates that neuritin may be associated with the growth and development of a variety of tissues and cells, and even involved in the pathogenesis of tumors.Gastric cancer demonstrates the highest incidence of all cancers worldwide, with ~900,000 novel patients and ~700,000 associated mortalities each year (10). In 2005, the incidence of gastric cancer in China was 37.1 cases per 100,000 males and 17.4 cases per 100,000 females (10). China is a region with a high incidence of gastric cancer, but the early detection rate for gastric cancer is only ~5% in China (10). The majority of patients admitted to hospital are in the advanced stage of gastric cancer and less than one-half of these patients survive for >5 years (11). Therefore, the identification of a more effective method for detecting early-stage gastric can...

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“…Yuan et al found that the rate of strong Neuritin expression in gastric cancer tissues (82.76%) was notably upregulated in comparison with that in the adjacent normal tissues, which was consistent with the results found in lung cancer, colon cancer, prostate cancer, glioblastoma, and other solid tumors 16,22,23. According to the previous study results, it is reasonable to speculate that Neuritin expression might be associated with NSCLC-VECs.…”

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Functions and the related signaling pathways of the neurotrophic factor neuritin

“…Although most studies regarding neuritin have focused primarily on its regulation and functions in neuroprotection and regeneration, other functions including roles in angiogenesis, tumorigenesis, and immunomodulation have also been reported. For example, new evidence has shown that neuritin expression can be down-regulated or up-regulated among different cancer types [3][4][5] . Another study found that recombinant neuritin protein increased human umbilical vein endothelial cell migration angiogenesis [6] .…”