Overexpression of mqsR in Xylella fastidiosa Leads to a Priming Effect of Cells to Copper Stress Tolerance

Carvalho, Isis Gabriela Barbosa; Merfa, Marcus V.; Teixeira-Silva, Natália Sousa; Martins, Paula Maria Moreira; Takita, Marco Aurélio; Souza, Alessandra Alves de

doi:10.3389/fmicb.2021.712564

Cited by 3 publications

(1 citation statement)

References 70 publications

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“…Another TA system, DarTG, in M. tuberculosis could catalyze reversible ADP-ribosylation of DNA for the SOS response ( 58 ). In Xylella fastidiosa , mqsR overexpression induced a series of genes to help bacterial survival under copper stress ( 59 ). Furthermore, the HigBA TA pair in P. aeruginosa was associated with pyocyanin production and antibiotic resistance ( 17 ).…”

Section: Discussionmentioning

confidence: 99%

The novel type II toxin–antitoxin PacTA modulates Pseudomonas aeruginosa iron homeostasis by obstructing the DNA-binding activity of Fur

Song

Zhang

et al. 2022

Nucleic Acids Research

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Type II toxin–antitoxin (TA) systems are widely distributed in bacterial and archaeal genomes and are involved in diverse critical cellular functions such as defense against phages, biofilm formation, persistence, and virulence. GCN5-related N-acetyltransferase (GNAT) toxin, with an acetyltransferase activity-dependent mechanism of translation inhibition, represents a relatively new and expanding family of type II TA toxins. We here describe a group of GNAT-Xre TA modules widely distributed among Pseudomonas species. We investigated PacTA (one of its members encoded by PA3270/PA3269) from Pseudomonas aeruginosa and demonstrated that the PacT toxin positively regulates iron acquisition in P. aeruginosa. Notably, other than arresting translation through acetylating aminoacyl-tRNAs, PacT can directly bind to Fur, a key ferric uptake regulator, to attenuate its DNA-binding affinity and thus permit the expression of downstream iron-acquisition-related genes. We further showed that the expression of the pacTA locus is upregulated in response to iron starvation and the absence of PacT causes biofilm formation defect, thereby attenuating pathogenesis. Overall, these findings reveal a novel regulatory mechanism of GNAT toxin that controls iron-uptake-related genes and contributes to bacterial virulence.

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Section: Discussionmentioning

confidence: 99%

The novel type II toxin–antitoxin PacTA modulates Pseudomonas aeruginosa iron homeostasis by obstructing the DNA-binding activity of Fur

Song

Zhang

et al. 2022

Nucleic Acids Research

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Threat of Xylella fastidiosa and options for mitigation in infected plants

Burbank

2022

CABI Reviews

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The bacterial pathogen Xylella fastidiosa continues to threaten agricultural production of many different crops around the world, with significant economic burden from crop loss, disease management, and surveillance costs. In addition to direct economic consequences, plant diseases caused by X. fastidiosa have had significant societal impacts in the most affected regions. Although X. fastidiosa infects long-term perennial crops and landscape plants, there has never been a truly effective treatment for plants once they become infected. This review discusses the threat of X. fastidiosa to agriculture, landscapes, and global commerce, in addition to the most recent progress in treatment for X. fastidiosa in infected plants. Current disease mitigation options include nutritional, chemical, biocontrol, and plant resistance-based strategies, with the potential to develop combined management approaches. Overall, several strategies are promising for disease suppression but there is still opportunity for innovation, especially in treatment approaches that can be administered systemically by soil or foliar spray routes. Prevention of severe disease outcomes and crop loss, and the ability to suppress pathogen populations and reduce transmission without heavy reliance on insecticides would have significant economic and environmental benefits.

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Toxin-Antitoxin System HigBA in Xylella fastidiosa Is Expressed at Low Temperature but Does Not Impact Virulence or Grapevine Recovery in Greenhouse Trials

Wei

Burbank

2023

PhytoFrontiers™

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Xylella fastidiosa has multiple chromosomally encoded type II toxin-antitoxin (TA) systems, several of which have unknown functions in the bacterial lifecycle. In many bacterial species, type II TA systems are associated with bacterial stress survival under various detrimental environmental conditions, as well as influencing disease associated phenotypes such as biofilm development and expression of virulence factors. Chromosomal TA systems are often found in genomic regions with evidence of phage insertion, pointing to potential acquisition of these elements by horizontal gene transfer. One of the TA systems found in X. fastidiosa, higBA is transcriptionally expressed at higher levels at 15°C than at 28°C. However, a higBA deletion mutant (ΔhigBA) in X. fastidiosa subsp. fastidiosa Stag’s Leap is not deficient in growth or viability under in vitro cold stress and is able to infect and cause disease in grapevines comparable to the wild type strain. Grapevines infected with X. fastidiosa ΔhigBA recover from infection following cold treatment at 4°C at a similar rate to wild type infected plants, suggesting this TA system does not contribute significantly to X. fastidiosa survival at low temperature in planta. Although a definitive function for HigBA in X. fastidiosa has yet to be determined, this study adds to a growing body of work describing functions and expression dynamics of TA systems in plant pathogenic bacteria.

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Overexpression of mqsR in Xylella fastidiosa Leads to a Priming Effect of Cells to Copper Stress Tolerance

Cited by 3 publications

References 70 publications

The novel type II toxin–antitoxin PacTA modulates Pseudomonas aeruginosa iron homeostasis by obstructing the DNA-binding activity of Fur

The novel type II toxin–antitoxin PacTA modulates Pseudomonas aeruginosa iron homeostasis by obstructing the DNA-binding activity of Fur

Threat of Xylella fastidiosa and options for mitigation in infected plants

Toxin-Antitoxin System HigBA in Xylella fastidiosa Is Expressed at Low Temperature but Does Not Impact Virulence or Grapevine Recovery in Greenhouse Trials

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