Overexpression of MpCYS2, a phytocystatin gene from Malus prunifolia (Willd.) Borkh., confers drought tolerance and protects against oxidative stress in Arabidopsis

Tan, Yanxiao; Li, Mingjun; Ma, Fengwang

doi:10.1007/s11240-015-0809-0

Cited by 13 publications

(8 citation statements)

References 57 publications

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“…Prior studies have shown that transgenic plants that have an expanded RSA have improved water homeostasis (Hetherington & Woodward, ; Malamy, ; Masle, Gilmore, & Farquhar, ; Yu et al, ). As a major environmental stress, drought imposes adverse effects on plant growth and development (Denby & Gehring, ; Liu, Qin, Han, Xiang, & Zhao, ; Tan, Li, & Ma, ). Plants need to reallocate energy to increase stress tolerance, and they adjust growth and development during stress‐inducing conditions (Ahuja, de Vos, Bones, & Hall, ; Skircyz & Inzé, ) using diverse mechanisms for coping at the morphological, anatomical, and cellular levels (Bray, ).…”

Section: Discussionmentioning

confidence: 99%

Ectopic expression of a pea apyrase enhances root system architecture and drought survival in Arabidopsis and soybean

Veerappa

Slocum

Siegenthaler

et al. 2018

Plant Cell & Environment

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Ectoapyrases (ecto-NTPDases) function to decrease levels of extracellular ATP and ADP in animals and plants. Prior studies showed that ectopic expression of a pea ectoapyrase, psNTP9, enhanced growth in Arabidopsis seedlings and that the overexpression of the two Arabidopsis apyrases most closely related to psNTP9 enhanced auxin transport and growth in Arabidopsis. These results predicted that ectopic expression of psNTP9 could promote a more extensive root system architecture (RSA) in Arabidopsis. We confirmed that transgenic Arabidopsis seedlings had longer primary roots, more lateral roots, and more and longer root hairs than wild-type plants. Because RSA influences water uptake, we tested whether the transgenic plants could tolerate osmotic stress and water deprivation better than wild-type plants, and we confirmed these properties. Transcriptomic analyses revealed gene expression changes in the transgenic plants that helped account for their enhanced RSA and improved drought tolerance. The effects of psNTP9 were not restricted to Arabidopsis, because its expression in soybeans improved the RSA, growth, and seed yield of this crop and supported higher survival in response to drought. Our results indicate that in both Arabidopsis and soybeans, the constitutive expression of psNTP9 results in a more extensive RSA and improved survival in drought stress conditions.

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Section: Discussionmentioning

confidence: 99%

Ectopic expression of a pea apyrase enhances root system architecture and drought survival in Arabidopsis and soybean

Veerappa

Slocum

Siegenthaler

et al. 2018

Plant Cell & Environment

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“…To examine transient expression, we transformed the plasmids into onion epidermal cells with a biolistic helium gun device (PDS-1000; BioRad, Hercules, CA, USA). Cell culturing and fluorescence observations were conducted as previously described 47 . These constructs were also transiently expressed in Arabidopsis protoplasts via polyethylene glycol-mediated transformation based on a protocol previously described 48 .…”

Section: Methodsmentioning

confidence: 99%

Increased activity of MdFRK2, a high-affinity fructokinase, leads to upregulation of sorbitol metabolism and downregulation of sucrose metabolism in apple leaves

et al. 2018

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To investigate the functions of fructokinase (FRK) in apple (Malus domestica) carbohydrate metabolism, we cloned the coding sequences of MdFRK1 and MdFRK2 from the ‘Royal Gala’ apple. The results showed that MdFRK2 expression was extremely high in shoot tips and young fruit. Analyses of heterologously expressed proteins revealed that MdFRK2 had a higher affinity for fructose than did MdFRK1, with Km values of 0.1 and 0.62 mM for MdFRK2 and MdFRK1, respectively. The two proteins, however, exhibited similar Vmax values when their activities were significantly inhibited by high concentrations of fructose. MdFRK2 ectopic expression was associated with a general decrease in fructose concentration in transgenic lines. In leaves, increased FRK activity similarly resulted in reduced concentrations of glucose and sucrose but no alterations in sorbitol concentration. When compared with those in the untransformed control, genes involved in sorbitol synthesis (A6PR) and the degradation pathway (SDH1/2) were significantly upregulated in transgenic lines, whereas those involved in sucrose synthesis (SPS1) and other degradation processes (SUSY4, NINV1/2, and HxK2) were downregulated. The activity of enzymes participating in carbohydrate metabolism was proportional to the level of gene expression. However, the growth performance and photosynthetic efficiency did not differ between the transgenic and wild-type plants. These results provide new genetic evidence to support the view that FRK plays roles in regulating sugar and sorbitol metabolism in Rosaceae plants.

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“…In a recent study, Yadav et al (2016) developed transgenic tobacco plants overexpressing the Arabidopsis annexin gene (AnnAt8) and demonstrated higher chlorophyll retention in transgenic plants than the WT after treatment with 300 mM and 400 mM NaCl. Tan et al (2015) also demonstrated higher chlorophyll content in transgenic Arabidopsis plants overexpressing a novel cystatin gene from Malus prunifolia (MpCYS5) when compared with the WT in the presence of 200 mM NaCl.…”

Section: Response Of T1 Progenies To Salt Stressmentioning

confidence: 92%

Characterization of proteinase inhibitor-II gene under OsRGLP2promoter for salt stress in transgenic Nicotiana benthamiana

Rehman¹,

Jørgensen²,

Rasmussen³

et al. 2017

Turk J Biol

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Proteinase inhibitors in plants are mainly involved in conferring resistance against herbivores and pathogens. However, their role in abiotic stress resistance has also been reported. Therefore, transgenic tobacco plants were generated constitutively expressing the tomato proteinase inhibitor-II (PI-II) under control of the rice root germin-like protein 2 (OsRGLP2) promoter using Agrobacterium tumefaciens. T1 transgenic seedlings were subjected to stress treatments with different concentrations of sodium chloride (NaCl) (100 mM, 200 mM, 300 mM). Quantitative real-time PCR revealed considerable levels of expression and upregulation of PI-II in transgenic lines under salt stress. Considering the phenotypic effects, the transgenic seedlings showed better growth, higher chlorophyll content, higher proline contents, and higher average fresh weight when compared to the wild type. This comparative analysis of transgenic and wild-type seedlings allows us to suggest that PI-II gene activity can be upregulated via the OsRGLP2 promoter under osmotic stress conditions.

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Overexpression of MpCYS2, a phytocystatin gene from Malus prunifolia (Willd.) Borkh., confers drought tolerance and protects against oxidative stress in Arabidopsis

Cited by 13 publications

References 57 publications

Ectopic expression of a pea apyrase enhances root system architecture and drought survival in Arabidopsis and soybean

Ectopic expression of a pea apyrase enhances root system architecture and drought survival in Arabidopsis and soybean

Increased activity of MdFRK2, a high-affinity fructokinase, leads to upregulation of sorbitol metabolism and downregulation of sucrose metabolism in apple leaves

Characterization of proteinase inhibitor-II gene under OsRGLP2promoter for salt stress in transgenic Nicotiana benthamiana

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