Overexpression of miR164b-resistant OsNAC2 improves plant architecture and grain yield in rice

Jiang, Dagang; Chen, Weiting; Dong, Jingfang; Li, Jing; Yang, Fen; Wu, Zhichao; Zhou, Hai; Wang, Wensheng; Zhuang, Chuxiong

doi:10.1093/jxb/ery017

Cited by 63 publications

(53 citation statements)

References 47 publications

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“…For example, the transgenic lines had a higher filling rate than that of the wild type plants under drought and heat stress conditions, resulting in increases in the total grain weight. These results were in consistent with that of previous studies which reported higher rice grain yield in rice plants overexpressing AP37 [66], TIFY [67], OsNAC2 [68], and OsNRT2.1 [69].…”

Section: Resultssupporting

confidence: 93%

Overexpression of Rice Rab7 Gene Improves Drought and Heat Tolerance and Increases Grain Yield in Rice (Oryza sativa L.)

El‐Esawi

Alayafi

2019

Genes

118

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Rab family proteins play a crucial role in plant developmental processes and tolerance to environmental stresses. The current study investigated whether rice Rab7 (OsRab7) overexpression could improve rice tolerance to drought and heat stress conditions. The OsRab7 gene was cloned and transformed into rice plants. The survival rate, relative water content, chlorophyll content, gas-exchange characteristics, soluble protein content, soluble sugar content, proline content, and activities of antioxidant enzymes (CAT, SOD, APX, POD) of the transgenic rice lines were significantly higher than that of the wild-type. In contrast, the levels of hydrogen peroxide, electrolyte leakage, and malondialdehyde of the transgenic lines were significantly reduced when compared to wild-type. Furthermore, the expression of four genes encoding reactive oxygen species (ROS)-scavenging enzymes (OsCATA, OsCATB, OsAPX2, OsSOD-Cu/Zn) and eight genes conferring abiotic stress tolerance (OsLEA3, OsRD29A, OsSNAC1, OsSNAC2, OsDREB2A, OsDREB2B, OsRAB16A, OsRAB16C) was significantly up-regulated in the transformed rice lines as compared to their expression in wild-type. OsRab7 overexpression also increased grain yield in rice. Taken together, the current study indicates that the OsRab7 gene improves grain yield and enhances drought and heat tolerance in transgenic rice by modulating osmolytes, antioxidants and abiotic stress-responsive genes expression. Therefore, OsRab7 gene could be exploited as a promising candidate for improving rice grain yield and stress tolerance.

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Section: Resultssupporting

confidence: 93%

Overexpression of Rice Rab7 Gene Improves Drought and Heat Tolerance and Increases Grain Yield in Rice (Oryza sativa L.)

El‐Esawi

Alayafi

2019

Genes

118

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“…For example, overexpressing miR444b.1 negatively regulates rice blast resistance with reduced tiller numbers (Xiao et al, 2017), whereas, overexpression of its target gene OsMADS57 resulted in increased number of tillers (Guo et al, 2013). Similarly, miR164a negatively regulates M. oryzae resistance by suppressing OsNAC60 (Wang et al, 2018), whereas overexpression of another miR164 target, OsNAC2 , leads to improvement of plant architecture and grain yield in rice (Jiang et al, 2018). In these reports, immunity and yield traits were regulated by different miRNA isoforms or different target genes.…”

Section: Discussionmentioning

confidence: 99%

miR396-OsGRFs Module Balances Growth and Rice Blast Disease-Resistance

et al. 2019

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Fitness cost is a common phenomenon in rice blast disease-resistance breeding. MiR396 is a highly conserved microRNA (miRNA) family targeting Growth Regulating Factor (OsGRF) genes. Mutation at the target site of miR396 in certain OsGRF gene or blocking miR396 expression leads to increased grain yield. Here we demonstrated that fitness cost can be trade-off in miR396-OsGRFs module via balancing growth and immunity against the blast fungus. The accumulation of miR396 isoforms was significantly increased in a susceptible accession, but fluctuated in a resistant accession upon infection of Magnaporthe oryzae. The transgenic lines over-expressing different miR396 isoforms were highly susceptible to M. oryzae. In contrast, overexpressing target mimicry of miR396 to block its function led to enhanced resistance to M. oryzae in addition to improved yield traits. Moreover, transgenic plants overexpressing OsGRF6, OsGRF7, OsGRF8, and OsGRF9 exhibited enhanced resistance to M. oryzae, but showed different alteration of growth. While overexpression of OsGRF7 led to defects in growth, overexpression of OsGRF6, OsGRF8, and OsGRF9 resulted in better or no significant change of yield traits. Collectively, our results indicate that miR396 negatively regulates rice blast disease- resistance via suppressing multiple OsGRFs, which in turn differentially control growth and yield. Therefore, miR396-OsGRFs could be a potential module to demolish fitness cost in rice blast disease-resistance breeding.

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“…Manipulation of miR398 can increase panicle length, grain number and grain size (Zhang et al ., 2017a) and suppressing expression of miR159 affects multiple traits of rice including grain shape by up‐regulating its targets OsGAMYB and OsGAMYBL1 (Zhao et al ., ). Overexpression of miR164b‐resistant target, OsNAC2, could make better plant architecture, longer panicles, more grain number and yield (Jiang et al ., ). However, so far there is little information showing the effect of miRNA on rice yield through affecting grain filling by negatively regulating its target.…”

Section: Introductionmentioning

confidence: 97%

miR1432‐OsACOT (Acyl‐CoA thioesterase) module determines grain yield via enhancing grain filling rate in rice

Zhao

Peng

Sun

et al. 2018

Plant Biotechnology Journal

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Rice grain filling rate contributes largely to grain productivity and accumulation of nutrients. MicroRNAs (miRNAs) are key regulators of development and physiology in plants and become a novel key target for engineering grain size and crop yield. However, there is little studies, so far, showing the miRNA regulation of grain filling and rice yield, in consequence. Here, we show that suppressed expression of rice miR1432 (STTM1432) significantly improves grain weight by enhancing grain filling rate and leads to an increase in overall grain yield up to 17.14% in a field trial. Molecular analysis identified rice Acyl-CoA thioesterase (OsACOT), which is conserved with ACOT13 in other species, as a major target of miR1432 by cleavage. Moreover, overexpression of miR1432-resistant form of OsACOT (OXmACOT) resembled the STTM1432 plants, that is, a large margin of an increase in grain weight up to 46.69% through improving the grain filling rate. Further study indicated that OsACOT was involved in biosynthesis of medium-chain fatty acids. In addition, RNA-seq based transcriptomic analyses of transgenic plants with altered expression of miR1432 demonstrated that downstream genes of miR1432-regulated network are involved in fatty acid metabolism and phytohormones biosynthesis and also overlap with the enrichment analysis of co-expressed genes of OsACOT, which is consistent with the increased levels of auxin and abscisic acid in STTM1432 and OXmACOT plants. Overall, miR1432-OsACOT module plays an important role in grain filling in rice, illustrating its capacity for engineering yield improvement in crops.

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Overexpression of miR164b-resistant OsNAC2 improves plant architecture and grain yield in rice

Abstract: Plant architecture affects grain yield. Overexpressing a version of OsNAC2 resistant to miRNA-mediated degradation increased panicle number and grain yield under field conditions, identifying a new strategy to improve yield.

Cited by 63 publications

References 47 publications

Overexpression of Rice Rab7 Gene Improves Drought and Heat Tolerance and Increases Grain Yield in Rice (Oryza sativa L.)

Overexpression of Rice Rab7 Gene Improves Drought and Heat Tolerance and Increases Grain Yield in Rice (Oryza sativa L.)

miR396-OsGRFs Module Balances Growth and Rice Blast Disease-Resistance

miR1432‐OsACOT (Acyl‐CoA thioesterase) module determines grain yield via enhancing grain filling rate in rice

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