Overexpression of miR-939-3p predicts poor prognosis and promotes progression in lung cancer

Han, Xiaodong; Du, Chenxing; Chen, Yinghai; Zhong, Xiaofei; Wang, Feng; Wang, Juan; Liu, Changmin; Li, Mianli; Chen, Shaoshui; Li, Baosheng

doi:10.3233/cbm-190271

Cited by 11 publications

(11 citation statements)

References 30 publications

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“…Similarly, miR-939-3p expression was up-regulated in lung cancer and associated with TNM stage, metastasis, and poor prognosis. 22 However, miR-939-5p expression in gastric and bladder cancer was decreased. 21,36 These data suggest that the expression and clinical correlation of miR-939 vary in different cancers.…”

Section: Discussionmentioning

confidence: 99%

“…24 miR-939-3p promoted cell invasion, migration and proliferation of lung cancer. 22 However, miR-939-5p suppressed invasion and migration of colorectal cancer cells through directly targeting LIMK2, 23 and miR-939 overexpression inhibited gastric cancer cell metastasis, proliferation and chemosensitivity through directly targeting SLC34A2. 21 Considering the expression of miR-939-5p is increased in pancreatic cancer tissues, we investigated whether miR-939-5p acts as an oncogene role in cell proliferation, migration, and invasion of pancreatic cancer.…”

Section: Discussionmentioning

confidence: 99%

“…17 In addition, the expression profile of miRNAs varies even in the different locations, 18 which further confirms that miRNAs have high application value in the diagnosis, prognosis evaluation and treatment of pancreatic cancer. Recently, a widely reported miRNA, miR-939, has been pointed out to be pivotal in the development and procession of cancers, such as hepatocellular carcinoma, 19 tongue squamous cell carcinoma, 20 gastric, 21 lung, 22 colorectal, 23 and ovarian cancer. 24 However, the clinical significances and roles of miR-939 in pancreatic cancer are still unknown.…”

Section: Introductionmentioning

confidence: 99%

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<p>miR-939-5p Contributes to the Migration and Invasion of Pancreatic Cancer by Targeting ARHGAP4</p>

Shen¹,

Chen²,

Gao³

et al. 2020

OTT

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Rho GTPase-activating protein 4 (ARHGAP4) is a GTPase-activating protein for the small GTPases of the Rho family that is involved in tumorigenesis. We recently reported that ARHGAP4 can mediate Warburg effect and malignant phenotype of pancreatic cancer. However, the regulation of ARHGAP4 remains unclear. Methods: ARHGAP4 and miR-939-5p expressions in pancreatic cancer tissues and cell lines were measured by real-time PCR or Western blotting. Pancreatic cancer cells were transfected with miR-939-5p inhibitor, miR-939-5p mimic and/or lentivirus expressing ARHGAP4, and the cell viability, invasion and migration were measured by CCK-8 and Transwell assay, respectively. The suppression of ARHGAP4 expression by miR-939-5p was revealed by luciferase reporter assay, real-time PCR or Western blotting. Results: ARHGAP4 expression was decreased, while miR-939-5p was increased in pancreatic cancer tissues compared with adjacent-normal pancreatic tissues. Higher miR-939-5p expression was correlated with advanced pathological stages and poor prognosis of pancreatic cancer patients. miR-939-5p directly targeted ARHGAP4. Either miR-939-5p down-regulation or ARHGAP4 overexpression inhibited viability, invasion and migration of pancreatic cancer cells. However, ARHGAP4 overexpression markedly inhibited the increased viability, migration, and invasion induced by miR-939-5p up-regulation in pancreatic cancer cells. Conclusion: These observations suggested that miR-939-5p regulates the malignant phenotype of pancreatic cancer cells by targeting ARHGAP4, establishing miR-939-5p as a novel regulator of ARHGAP4 with a critical role in tumorigenesis in pancreatic cancer.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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<p>miR-939-5p Contributes to the Migration and Invasion of Pancreatic Cancer by Targeting ARHGAP4</p>

Shen¹,

Chen²,

Gao³

et al. 2020

OTT

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“…In liver cancer patient survival prediction, multi-omics data have shown better performance than single-omics data for model building (37). Among the aforementioned 16 miRNAs, the overexpression of hsa-miR-939 was correlated with poor prognosis in lung cancer (38), as well as promoting epithelial to mesenchymal transition in epithelial ovarian cancer (39). It has been reported that hsa-miR421 promotes tumor progression in hepatocellular carcinoma (40) and osteosarcoma (41), and hsa-miR-335 participates in the progression of gallbladder carcinoma (42).…”

Section: Discussionmentioning

confidence: 99%

A novel microRNA signature for pathological grading in�lung adenocarcinoma based on TCGA and GEO data

Yang

Yin²,

Shi

et al. 2020

Int J Mol Med

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Lung adenocarcinoma (LUAd) is one of the most common types of lung cancer and its poor prognosis largely depends on the tumor pathological stage. critical roles of microRNAs (miRNAs) have been reported in the tumorigenesis and progression of lung cancer. However, whether the differential expression pattern of miRNAs could be used to distinguish early-stage (stage I) from mid-late-stage (stages II-IV) LUAd tumors is still unclear. In this study, clinical information and miRNA expression profiles of patients with LUAd were downloaded from The cancer Genome Atlas (TcGA) and Gene Expression Omnibus databases. TcGA-LUAd (n=470) dataset was used for model training and validation, and the GSE62182 (n=94) and GSE83527 (n=36) datasets were used as external independent test datasets. The diagnostic model was created through miRNA feature selection followed by SVM classifier and was confirmed by 5-fold cross-validation. A receiver operating characteristic curve was calculated to evaluate the accuracy and robustness of the model. Using the dX score and LIBSVM tool, a 16-miRNA signature that could distinguish LUAd pathological stages was identified. The area under the curve rates were 0.62 [95% confidence interval (

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“…Meanwhile, miRNAs participate in the regulation of a variety of human proteins, which makes it easier to affect the genetic pathways [5]. Currently, miRNA has been reported to act as oncogenes or antioncogenes in the initiation and development of various cancers [6][7][8][9][10]. For example, miR-423-3p acts as an oncogene and promotes cell proliferation, migration, and invasion of lung cancer [11].…”

Section: Introductionmentioning

confidence: 99%

Downregulation of miR-484 is associated with poor prognosis and tumor progression of gastric cancer

Yao

et al. 2020

Diagn Pathol

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Background: Gastric cancer is one of the most common cancers leading to high cancer mortality. MicroRNA-484 (miR-484) has been evaluated as a biomarker for various types of cancers. The subject of this study is to investigate the functional role of miR-484 in gastric cancer. Methods: The expression of miR-484 in gastric cancer was analyzed by quantitative real-time polymerase chain reaction (qRT-PCR) assay. Kaplan-Meier survival and Cox regression analyses were employed to explore the prognostic significance of miR-484 in gastric cancer. The functional role of miR-484 in gastric cancer was determined by CCK-8 and Transwell assays. Results: The results showed that miR-484 was significantly downregulated in gastric cancer tissues and cell lines. The downregulation of miR-484 was closely related to differentiation, lymph node metastasis, TNM stage, and poor prognosis. Cox regression analyses demonstrated that miR-484 was an independent prognosis indicator for gastric cancer patients. Additionally, the downregulation of miR-484 enhanced cell proliferation, migration, and invasion in gastric cancer cells. Conclusion: These data demonstrated that miR-484 can serve as a potential prognostic biomarker and therapeutic target for gastric cancer and it may be involved in the progression of gastric cancer.

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Overexpression of miR-939-3p predicts poor prognosis and promotes progression in lung cancer

Cited by 11 publications

References 30 publications

<p>miR-939-5p Contributes to the Migration and Invasion of Pancreatic Cancer by Targeting ARHGAP4</p>

<p>miR-939-5p Contributes to the Migration and Invasion of Pancreatic Cancer by Targeting ARHGAP4</p>

A novel microRNA signature for pathological grading in�lung adenocarcinoma based on TCGA and GEO data

Downregulation of miR-484 is associated with poor prognosis and tumor progression of gastric cancer

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