Overexpression of miR-651-5p inhibits ultraviolet radiation-induced malignant biological behaviors of sebaceous gland carcinoma cells by targeting ZEB2

Zhao, Hang; Yang, Xiaochun; Liu, Juan; Han, Fang; Yang, Zheng; Hu, Zhongyin; Liu, Mingzhi; Yan, Mi

doi:10.21037/atm-21-3897

Cited by 4 publications

(4 citation statements)

References 60 publications

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“…Also, a study has reported that overexpression of miR‐651‐5p suppresses SGC cell malignant actions. More specifically, miR‐651‐5p not only promotes cell apoptosis but also inhibits the invasive and migratory ability and epithelial‐mesenchymal transition of ultraviolet‐induced SGC cells 8 . Collectively, our study findings are in conformity with these study outcomes.…”

Section: Discussionsupporting

confidence: 88%

“…miRNAs are good candidates as biomarkers for lung cancer 7 . For example, miR‐651‐5p expression is associated with sebaceous gland carcinoma (SGC) malignant behaviors 8 . It is also suggested that miR‐651‐5p expression has an association with colorectal cancer (CRC) 9 .…”

Section: Introductionmentioning

confidence: 99%

“…7 For example, miR-651-5p expression is associated with sebaceous gland carcinoma (SGC) malignant behaviors. 8 It is also suggested that miR-651-5p expression has an association with colorectal cancer (CRC). 9 A previous study has demonstrated that miR-651-5p-mediated oncogenicity manipulation in hepatocellular carcinoma (HCC) may provide guiding significance on the diagnosis and therapy for HCC.…”

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confidence: 99%

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microRNA‐651‐5p affects the proliferation, migration, and invasion of lung cancer cells by regulating Calmodulin 2 expression

Lang

Kong

Liu

et al. 2023

Clinical Respiratory J

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ObjectiveLung cancer is prevalent worldwide and a leading contributor to tumor death. This research intends to explore the molecular mechanism of the microRNA‐651‐5p (miR‐651‐5p)/Calmodulin 2 (CALM2) axis in the proliferation, migration, and invasion of lung cancer cells.MethodsLung cancer tissues and para‐cancerous tissues were collected. The expression levels of miR‐651‐5p and CALM2 in lung cancer tissues and cells were tested, and the connection between miR‐651‐5p expression and clinicopathological characteristics of lung cancer patients was further analyzed. The binding sites between miR‐651‐5p and CALM2 were analyzed and validated. Lung cancer cell proliferation, migration, invasion, and apoptosis were examined.ResultsmiR‐651‐5p was lowly expressed in lung cancer tissues and cells. miR‐651‐5p expression had no correlation with patients' age and gender but had a correlation with patients' tumor size, TNM stage, and lymph node metastasis. Overexpression of miR‐651‐5p repressed proliferative, migratory, and invasive behaviors of lung cancer cells. miR‐651‐5p targeted and negatively regulated CALM2 expression, and CALM2 reversed the inhibiting effects of miR‐651‐5p on lung cancer cell malignant behaviors, including proliferation, migration, and invasion.ConclusionThis study expounds that miR‐651‐5p affects the proliferation, migration, and invasion of lung cancer cells by regulating CALM2 expression.

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Section: Discussionsupporting

confidence: 88%

Section: Introductionmentioning

confidence: 99%

mentioning

confidence: 99%

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microRNA‐651‐5p affects the proliferation, migration, and invasion of lung cancer cells by regulating Calmodulin 2 expression

Lang

Kong

Liu

et al. 2023

Clinical Respiratory J

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“…This article ( 1 ) titled “Overexpression of miR-651-5p inhibits ultraviolet radiation-induced malignant biological behaviors of sebaceous gland carcinoma cells by targeting ZEB2 ” (doi: 10.21037/atm-21-3897), unfortunately contain errors in the authorship section, the legend of Figure 2, Figure 3 and its legend, Figure 4, Figure 5 and its legend, and the legend of Figure 7.…”

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confidence: 99%

Erratum to overexpression of miR-651-5p inhibits ultraviolet radiation-induced malignant biological behaviors of sebaceous gland carcinoma cells by targeting ZEB2

2023

Ann Transl Med

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Establishment and Characterization of Three Human Ocular Adnexal Sebaceous Carcinoma Cell Lines

Lee,

Peterson,

Wang

et al. 2024

IJMS

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Ocular adnexal sebaceous carcinoma (SebCA) represents one of the most clinically problematic periocular tumors, often requiring aggressive surgical resection. The pathobiology of this tumor remains poorly understood, and few models exist that are suitable for preclinical testing. The aim of this study was to establish new cell lines to serve as models for pathobiological and drug testing. With patient consent, freshly resected tumor tissue was cultured using conditional reprogramming cell conditions. Standard techniques were used to characterize the cell lines in terms of overall growth, clonogenicity, apoptosis, and differentiation in vitro. Additional analyses including Western blotting, short tandem repeat (STR) profiling, and next-generation sequencing (NGS) were performed. Drug screening using mitomycin-C (MMC), 5-fluorouricil (5-FU), and 6-Diazo-5-oxo-L-norleucine (DON) were performed. JHH-SebCA01, JHH-SebCA02, and JHH-SebCA03 cell lines were established from two women and one man undergoing surgical resection of eyelid tumors. At passage 15, they each showed a doubling time of two to three days, and all could form colonies in anchorage-dependent conditions, but not in soft agar. The cells contained cytoplasmic vacuoles consistent with sebaceous differentiation, and adipophilin protein was present in all three lines. STR profiling confirmed that all lines were derived from their respective patients. NGS of the primary tumors and their matched cell lines identified numerous shared mutations, including alterations similar to those previously described in SebCA. Treatment with MMC or 5-FU resulted in dose-dependent growth inhibition and the induction of both apoptosis and differentiation. MYC protein was abundant in all three lines, and the glutamine metabolism inhibitor DON, previously shown to target high MYC tumors, slowed the growth of all our SebCA models. Ocular adnexal SebCA cell lines can be established using conditional reprogramming cell conditions, and our three new models are useful for testing therapies and interrogating the functional role of MYC and other possible molecular drivers. Current topical chemotherapies promote both apoptosis and differentiation in SebCA cells, and these tumors appear sensitive to inhibition or MYC-associated metabolic changes.

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Overexpression of miR-651-5p inhibits ultraviolet radiation-induced malignant biological behaviors of sebaceous gland carcinoma cells by targeting ZEB2

Cited by 4 publications

References 60 publications

microRNA‐651‐5p affects the proliferation, migration, and invasion of lung cancer cells by regulating Calmodulin 2 expression

microRNA‐651‐5p affects the proliferation, migration, and invasion of lung cancer cells by regulating Calmodulin 2 expression

Erratum to overexpression of miR-651-5p inhibits ultraviolet radiation-induced malignant biological behaviors of sebaceous gland carcinoma cells by targeting ZEB2

Establishment and Characterization of Three Human Ocular Adnexal Sebaceous Carcinoma Cell Lines

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