Overexpression of miR-595 and miR-1246 in the Sera of Patients with Active Forms of Inflammatory Bowel Disease

Krissansen, Geoffrey W.; Yang, Yi; McQueen, Fiona; Leung, Euphemia; Peek, Deborah; Chan, Yih Chih; Print, Cristin G.; Dalbeth, Nicola; Williams, Megan; Fraser, Alan G.

doi:10.1097/mib.0000000000000285

Cited by 47 publications

(40 citation statements)

References 69 publications

(88 reference statements)

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“…It is also known that miR‐595 and miR‐1246 were significantly up‐regulated in the serum of active colonic Crohn's disease, ulcerative colitis, and rheumatoid arthritis patients, compared with healthy subjects (Krissansen et al. ). In our findings, periodontitis up‐regulated expression rate of miR‐207, miR‐376‐3p, and miR‐495 in serum.…”

Section: Discussionmentioning

confidence: 99%

“…For instance, it is reported that serum levels of miR-20a, miR-28-3p, miR-34c-5p, and miR-100 were found to be significantly down-regulated in chronic obstructive pulmonary disease (Akbas et al 2012). It is also known that miR-595 and miR-1246 were significantly up-regulated in the serum of active colonic Crohn's disease, ulcerative colitis, and rheumatoid arthritis patients, compared with healthy subjects (Krissansen et al 2015). In our findings, periodontitis up-regulated expression rate of miR-207, miR-376-3p, and miR-495 in serum.…”

Section: Discussionmentioning

confidence: 99%

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MicroRNAs as serum biomarkers for periodontitis

Tomofuji

Yoneda

Machida

et al. 2016

J Clinic Periodontology

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

MicroRNAs as serum biomarkers for periodontitis

Tomofuji

Yoneda

Machida

et al. 2016

J Clinic Periodontology

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“…The downregulation of this gene could perturb its role in vessel remodelling and could be linked to vascular thrombosis in placenta of patient with APS. While for miR-595, it was previously found to be overexpressed in the sera of patients with inflammatory bowel disease [19]. As inflammatory-mediated tissue damage is also indicated in placenta of patients with APS, miR-595 could possibly involve in the mechanism of this disease.…”

Section: Discussionmentioning

confidence: 99%

MicroRNA and Gene Expression Analysis on Placenta Tissue: An Approach to Understanding Obstetric Antiphospholipid Syndrome at the Molecular Level

Asnawi

Mohamad

Manzor

et al. 2019

Preprint

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Obstetric antiphospholipid syndrome is initiated by the action of antiphospholipid antibodies on placenta. The characteristics of APS in pregnancy include vascular thrombosis, inflammation and impairment of trophoblast implantation. MicroRNA (miRNA) expression has been suggested as one of the genetic factors that contribute to the development of this syndrome. miRNAs regulate gene expressions in a vast assortment of cellular biological mechanisms include the development of placental tissue. Hence, further investigation on the regulation of placental miRNA in APS is required. In this study, we aimed to profile miRNA expressions from placenta tissue of patients with APS. Differentially expressed miRNAs were determined for its targeted genes and pathways. Agilent microarray platform was used to measure placental microRNA expressions between normal placental tissue and those obtained from patients with APS. Differentially expressed miRNAs were detected using GeneSpring GX software 14.2 and sequences were mapped using TargetScan software to generate the predicted target genes. Pathway analysis for the genes was then performed on PANTHER and REACTOME software. Selected miRNAs and their associated genes of interest were validated using qPCR. Microarray findings revealed, 9 downregulated and 21 upregulated miRNAs expressed in placenta of patients with APS. Quantitative expressions of 3 selected miRNAs were in agreement with the microarray findings, however only miR-525-5p expression was statistically significant. Pathway analysis revealed that the targeted genes of differentially expressed miRNAs were involved in several hypothesized signalling pathways such as the vascular endothelial (VE) growth factor (VEGF) and inflammatory pathways. VE-cadherin, ras homolog member A (RHOA) and tyrosine kinase receptor (KIT) showed significant downregulation from the qPCR data while retinoblastoma gene (RET), dual specificity protein phosphatase 10 (DUSP10) and B-lymphocyte kinase (BLK) were significantly upregulated. These preliminary findings suggest the involvement of miRNAs and identified novel associated genes involvement in the mechanism of obstetric APS, particularly through the alteration of vascular-associated regulators and the inflammatory signalling cascade.

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“…Overexpression of miR-595 promoted the glioblastoma cell proliferation and colony formation through targeting SOX7. Another study demonstrated that miR-595 expression was upregulated in the sera of active forms of inflammatory bowel disease patients [32]. Chen et al [31].…”

Section: Discussionmentioning

confidence: 99%

MicroRNA-595 sensitizes ovarian cancer cells to cisplatin by targeting ABCB1

et al. 2016

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Ovarian cancer is among the leading cause of cancer-related deaths in females. In this study, we demonstrated that miR-595 expression was downregulated in the ovarian cancer tissues and cell lines. miR-595 expression was lower in the lymph node metastases tissues than in the primary ovarian cancer tissues and normal tissues. Furthermore, miR-595 overexpression suppressed the ovarian cancer cell proliferation, colony formation and invasion and promoted the sensitivity of ovarian cancer cell to cisplatin. We identified ABCB1 as a direct target gene of miR-595 in the ovarian cancer cell. ABCB1 expression was upregulated in the ovarian cancer tissues and cell lines. Morevoer, the expression level of ABCB1 was inversely correlated with miR-595 in the ovarian cancer tissues. In addition, overexpression of ABCB1 decreased the miR-595-overexpressing HO8910PM and SKOV-3 cell sensitivity to cisplatin. Ectopic expression of ABCB1 promoted the miR-595-overexpressing HO8910PM and SKOV-3 cell proliferation, colony formation and invasion. These data suggested that miR-595 acted a tumor suppressor role in ovarian cancer development and increased the sensitivity of ovarian cancer to cisplatin.

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Overexpression of miR-595 and miR-1246 in the Sera of Patients with Active Forms of Inflammatory Bowel Disease

Cited by 47 publications

References 69 publications

MicroRNAs as serum biomarkers for periodontitis

MicroRNAs as serum biomarkers for periodontitis

MicroRNA and Gene Expression Analysis on Placenta Tissue: An Approach to Understanding Obstetric Antiphospholipid Syndrome at the Molecular Level

MicroRNA-595 sensitizes ovarian cancer cells to cisplatin by targeting ABCB1

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