Overexpression of miR‑361‑5p plays an oncogenic role in human lung adenocarcinoma through the regulation of SMAD2

Othman, Norahayu; Nagoor, Noor Hasima

doi:10.3892/ijo.2018.4602

Cited by 6 publications

(7 citation statements)

References 37 publications

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“…The role of miRNAs as tumor-inducers or -suppressors depends on their targets in numerous types of cancer. As previously reported, the dysregulation of miRNAs promotes tumor occurrence and development (8). Accumulating evidence supports the involvement of miR-340 in a variety of cancer types (9-11).…”

Section: Introductionsupporting

confidence: 56%

Effects of miR‑340 overexpression and knockdown on the proliferation and metastasis of NSCLC cell lines

et al. 2019

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The present study aimed to investigate the potential biological functions of microRNA-340 (miR-340) in non-small cell lung cancer (NScLc) beyond its role as a critical regulator of tumorigenesis and tumor progression. The expression levels of miR-340 and RAB27B were analyzed by reverse transcription-quantitative polymerase chain reaction. Subsequently, the protein expression levels of RAB27A, RAB27B, RAB9A, RAB11A and BRAB21 were determined by western blot analysis. The expression levels of the aforementioned proteins in NScLc tissues were analyzed by immunohistochemistry. RAB27B, as a potential target of miR-340 was investigated via a dual-luciferase reporter assay. The proliferative ability of Pc9, A549 and BEAS-2B cells was detected with a cell counting kit-8 assay, while the migration and invasion of the NScLc cells were analyzed using a Transwell assay. The results revealed that the expression levels of miR-340 in the NScLc cells were significantly decreased compared with those in normal cells (BEAS-2B cells). RAB27B was proposed as a potential target gene of miR-340, and its expression was notably increased in the NScLc cells. miR-340 overexpression inhibited the migration and invasion of the NScLc cells by targeting RAB27B, while the knockdown of miR-340 exerted opposite effects. On the whole, these findings indicate that the miR-340/RAB27B axis may be actively involved in the occurrence of NScLc. Thus, miR-340 and RAB27B may be novel therapeutic targets for the treatment of NScLc.

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Section: Introductionsupporting

confidence: 56%

Effects of miR‑340 overexpression and knockdown on the proliferation and metastasis of NSCLC cell lines

et al. 2019

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“…Therefore, we speculated that miRNA-190a-5p might serve as a biomarker in the early stage of POF. Usually, we also know that miRNAs exert their functions by degrading mRNAs expression ( Othman and Nagoor, 2019 ; Tian et al, 2019 ). Hence, we used the publicly available databases to seek the potential targets of miRNA-190a-5p.…”

Section: Discussionmentioning

confidence: 99%

MiRNA-190a-5p promotes primordial follicle hyperactivation by targeting PHLPP1 in premature ovarian failure

Zhang

Han

et al. 2022

Front. Genet.

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We previously screened 6 differentially expressed miRNAs in ovarian tissues of 4-vinylcyclohexene diepoxide (VCD)-treated premature ovarian failure (POF) model in SD rats, including miRNA-190a-5p, miRNA-98-5p, miRNA-29a-3p, miRNA-144-5p, miRNA-27b-3p, miRNA-151-5p. In this study, to investigate the mechanisms causing the onset of POF, we first identified miRNAs with earlier differential expression at consecutive time points in the VCD-treated rat POF model and explored the mechanisms by which the target miRNAs promote POF. The SD rats were injected with VCD for 15 days to induce POF. Additionally, we collected rat blood and ovaries at the same time every day for 15 consecutive days, and luteinizing hormone (LH), follicle-stimulating hormone (FSH), Anti-Mullerian hormone (AMH), and estradiol (E2) serum levels were detected by ELISA. Six miRNAs expression were measured in rat ovaries by qRT-PCR. Dual-luciferase reporter gene assays were employed to predict and verify the target gene (PHLPP1) of target miRNAs (miRNA-190a-5p). Western blot was examined to detect the expression levels of PHLPP1, AKT, p-AKT, FOXO3a, p-FOXO3a, and LHR proteins on the target gene PHLPP1 and its participation in the primordial follicular hyperactivation-related pathways (AKT-FOXO3a and AKT-LH/LHR). During the VCD modeling POF rat ovaries, miRNA-190a-5p was the first to show significant differential expression, i.e., 6th of VCD treating, and PHLPP1 was verified to be a direct downstream target of it. Starting from the 6th of VCD treatment, the more significant the up-regulation trend of miRNA-190a-5p expression, the more obvious the down-regulation trend of PHLPP1 and LHR mRNA and protein expression, accompanied by the more severe phosphorylation of AKT and FOXO3a proteins, thus continuously over-activating the rat primordial follicle to promote the development of POF. In conclusion, miRNA-190a-5p may become a potential biomarker for early screening of POF, and it can continuously activate primordial follicles in rats by targeting the expression of PHLPP1 and key proteins in the AKT-FOXO3a and AKT-LH/LHR pathways.

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“…In addition, some of the miRNAs identified using our analysis including miR‐450b‐5p, miR‐199s, and miR‐361‐5p, whose expressions were upregulated in all three categories, may act as an oncomiR or a tumor suppressor in certain types of tumors although their functions are ambiguous in pediatric HCC, implying a contextual role of these miRNAs. 99 , 100 , 101 , 102 , 103 , 104 , 105 , 106 , 107 , 108 , 109 , 110 , 111 , 112 , 113 , 114 , 115 , 116 , 117 , 118 , 119 , 120 , 121 Given the relatively small number of samples analyzed in the current study and the heterogeneous nature of clinical variables, future experimental investigation of candidate miRNAs would require a model system recapitulating a specific subtype of pediatric HCC. Of note, ToppMiR uses all seven sources of miRNA target predictions, two of which are based on experimentally validated targets 6 , 12 , 13 , 14 , 15 , 16 , 17 , 18 , 19 , 20 , 21 , 22 , 23 , 24 , 25 , 26 ; thus, targets identified by ToppMiR may not be from experimentally verified miRNA sources.…”

Section: Discussionmentioning

confidence: 99%

Integrated analysis using ToppMiR uncovers altered miRNA– mRNA regulatory networks in pediatric hepatocellular carcinoma—A pilot study

et al. 2022

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Background Pediatric hepatocellular carcinoma (HCC) is a group of liver cancers whose mechanisms behind their pathogenesis and progression are poorly understood. Aim We aimed to identify alterations in the expression of miRNAs and their putative target mRNAs in not only tumor tissues of patients with pediatric HCC but also in corresponding non‐tumorous background livers by using liver tissues without underlying liver disease as a control. Methods and results We performed a small‐scale miRNA and mRNA profiling of pediatric HCC (consisting of fibrolamellar carcinoma [FLC] and non‐FLC HCC) and paired liver tissues to identify miRNAs whose expression levels differed significantly from control livers without underlying liver disease. ToppMiR was used to prioritize both miRNAs and their putative target mRNAs in a gene‐annotation network, and the mRNA profile was used to refine the prioritization. Our analysis generated prioritized lists of miRNAs and mRNAs from the following three sets of analyses: (a) pediatric HCC versus control; (b) FLC versus control; and (c) corresponding non‐tumorous background liver tissues from the same patients with pediatric HCC versus control. No liver disease liver tissues were used as the control group for all analyses. Many miRNAs whose expressions were deregulated in pediatric HCC were consistent with their roles in adult HCC and/or other non‐hepatic cancers. Our gene ontology analysis of target mRNAs revealed enrichment of biological processes related to the sustenance and propagation of cancer and significant downregulation of metabolic processes. Conclusion Our pilot study indicates that alterations in miRNA–mRNA networks were detected in not only tumor tissues but also corresponding non‐tumorous liver tissues from patients with pediatric HCC, suggesting multi‐faceted roles of miRNAs in disease progression. Our results may lead to novel hypotheses for future large‐scale studies.

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Overexpression of miR‑361‑5p plays an oncogenic role in human lung adenocarcinoma through the regulation of SMAD2

Cited by 6 publications

References 37 publications

Effects of miR‑340 overexpression and knockdown on the proliferation and metastasis of NSCLC cell lines

Effects of miR‑340 overexpression and knockdown on the proliferation and metastasis of NSCLC cell lines

MiRNA-190a-5p promotes primordial follicle hyperactivation by targeting PHLPP1 in premature ovarian failure

Integrated analysis using ToppMiR uncovers altered miRNA– mRNA regulatory networks in pediatric hepatocellular carcinoma—A pilot study

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