Overexpression of Lymphocyte Antigen 6 Complex, Locus E in Gastric Cancer Promotes Cancer Cell Growth and Metastasis

Abstract

Background/Aims: Lymphocyte antigen 6 complex, locus E (LY6E) is a member of the lymphostromal cell membrane Ly6 superfamily protein. The present study investigated the clinical significance and potential biological function of LY6E in gastric cancer (GC). Methods: LY6E mRNA and protein expressions in human GC tissues and GC cells were tested. Relationship between LY6E expression and the GC patients’ clinicopathologic characteristics was analyzed. LY6E was silenced by siRNA in the cultured GC cells. Results: The RNA expression microarray profiling assay results demonstrated that LY6E mRNA was significantly increased in multiple human GC tumor tissues. Immunohistochemistry (IHC) staining analysis revealed that 59 of 75 (78.7%) GC specimens were LY6E positive. LY6E over-expression in human GC was correlated with the histology grade, AJCC stage, N classification, lymphatic invasion, and tumor location. Notably, functional LY6E expression was also detected in AGS and other established GC cell lines. LY6E knockdown by targeted-siRNA inhibited AGS cell survival and proliferation. Meanwhile, the LY6E siRNA induced G1-S cell cycle arrest and apoptosis in AGC cells. Additionally, AGC cell migration was also inhibited by LY6E knockdown. Expressions of tumor-suppressing proteins, including PTEN (phosphatase and tensin homolog) and E-Cadherin, were increased in LY6E-silenced AGS cells. Conclusion: LY6E over-expression in GC is potentially required for cancer cell survival, proliferation and migration.

Molecular prognosticators in clinically and pathologically distinct cohorts of head and neck squamous cell carcinoma—A meta-analysis approach

“…Differential expression profiling of the tongue cancer cohort triaged based on the stage, pathology identified multiple candidate markers; JAM2, SMURF1, LY6E, MFN1 and SUPT16H. JAM2 is reported to be instrumental for metastatic progression in breast and colon cancer [38,39], while others are known regulators of migratory/invasive properties in many cancers [31,[40][41][42][43][44]. Analysis in laryngopharyngeal cohort also identified markers of early stage disease with PNI/ALI which included MFN1, PAK2, PIK3CA, FXR1 (differentials), PRRC2B and CBLL1 a significantly upregulation in oropharyngeal HPV+ cases (B-F).…”

Emerging Role of LY6E in Virus–Host Interactions

“…Given that NF-κB is known to be essential for the lentiviral transcription [48,49], it is reasonable to speculate that LY6E may affect HIV-1 gene expression by interfering with the NF-κB level in virus-infected cells. It should be noted that although LY6E is a plasma membrane-targeting protein, it is also expressed intracellularly, including on intracellular membranes as examined by immunofluorescence staining [24,28,50]. In this respect, it will be informative to explore how this portion of LY6E may physically interact with transcription factors, thus participating in the modulation of viral and cellular gene expression.…”

Lnc-THOR silencing inhibits human glioma cell survival by activating MAGEA6-AMPK signaling

“…After incubation for 10 days, A172 cell colonies were stained and manually counted. The detailed protocol for the 5-ethynyl-2’-deoxyuridine (EdU) staining assay was reported earlier 32 .…”