Overexpression of long noncoding RNA MCM3AP-AS1 promotes osteogenic differentiation of dental pulp stem cells via miR-143-3p/IGFBP5 axis

Yang, Changwei; Xu, Xuehong; Lin, Ping-Ting; Luo, Bizhu; Luo, Shufang; Huang, Honglan; Zhu, Jianyu; Huang, Meie; Peng, Shuhai; Wu, Qianju; Yin, Lu

doi:10.1007/s13577-021-00648-3

Cited by 10 publications

(5 citation statements)

References 35 publications

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“…In a prior study, it was found that miR-143-3p hindered early HFF proliferation [21]. Evidence has indicated that miR-143-3p/IGFBP was shown to be involved in the osteogenic differentiation of dental pulp stem cells [42]. Additionally, testosterone could stimulate the secretion of IGFBP-3 in HFF [30].…”

Section: Testosterone Regulated the Expression Of Mir-143-3p And Igfb...mentioning

confidence: 97%

Testosterone promotes human foreskin fibroblast growth through miR-143-3p targeting IGFBP-3

2023

Journal of Men's Health

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Testosterone is an important male hormone, which could improve the maintenance and recovery of gonadal function in males as well as the repair of human hypospadias and cell fibrosis. Our study focused on investigating the regulatory effect of testosterone in human foreskin fibroblasts (HFF-1) and regulatory mechanisms involved. In this study, HFF-1 cells were treated with testosterone, and cell viability and migration were assessed by cell counting kit-8 (CCK8) and Transwell assays. The expression levels of androgen receptor (AR), miR-143-3p and insulin-like growth factor binding protein-3 (IGFBP-3) were measured by quantitative real-time PCR (qRT-PCR), Western blotting, and immunofluorescence. In addition, a potential binding site for miR-143-3p on IGFBP-3 was predicted and its direct binding was further confirmed by a dual luciferase reporter assay. These results showed that testosterone increased the viability and migration of HFF-1 cells. Testosterone could down-regulate miR-143-3p and up-regulate IGFBP-3 and AR. Overexpression of miR-143-3p hindered HFF-1 cell viability and negatively regulated IGFBP-3, whereas inhibition of IGFBP-3 impeded cell viability and migration. Furthermore, miR-143-3p was found to directly bind to IGFBP-3. Overexpression of IGFBP-3 countered the regulation of HFF-1 cells by miR-143-3p mimics. In conclusion, this study showed that testosterone promoted the proliferation and migration of HFF-1 cells and AR signaling, at least via the miR-143-3p/IGFBP-3 axis. This discovery presents a novel insight for testosterone application in male disorders like hypospadias.

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Section: Testosterone Regulated the Expression Of Mir-143-3p And Igfb...mentioning

confidence: 97%

Testosterone promotes human foreskin fibroblast growth through miR-143-3p targeting IGFBP-3

2023

Journal of Men's Health

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“…The absence of BSP and dentin sialo phosphoprotein (DSPP) was detected in DPSCs culture, which could indirectly reflect the absence of differentiation [ 14 , 22 ]. Under the influence of an osteogenic medium, DPSCs can induce the formation of mineralized nodules [ 69 ]. ALP is an early indicator of osteoblast differentiation and participates in the formation, metabolism, and regeneration of calcified tissues such as bone.…”

Section: Osteogenic Differentiation Abilitymentioning

confidence: 99%

“…[14,22]. Under the influence of an osteogenic medium, DPSCs can induce the formation of mineralized nodules [69]. ALP is an early indicator of osteoblast differentiation and participates in the formation, metabolism, and regeneration of calcified tissues such as bone.…”

Section: Osteogenic Differentiation Abilitymentioning

confidence: 99%

Dental Pulp Stem Cells for Bone Tissue Engineering: A Literature Review

Bai,

Cao,

et al. 2023

Stem Cells International

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Bone tissue engineering (BTE) is a promising approach for repairing and regenerating damaged bone tissue, using stem cells and scaffold structures. Among various stem cell sources, dental pulp stem cells (DPSCs) have emerged as a potential candidate due to their multipotential capabilities, ability to undergo osteogenic differentiation, low immunogenicity, and ease of isolation. This article reviews the biological characteristics of DPSCs, their potential for BTE, and the underlying transcription factors and signaling pathways involved in osteogenic differentiation; it also highlights the application of DPSCs in inducing scaffold tissues for bone regeneration and summarizes animal and clinical studies conducted in this field. This review demonstrates the potential of DPSC-based BTE for effective bone repair and regeneration, with implications for clinical translation.

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“…Many investigators have found that the abnormal expression of long noncoding RNAs (lncRNAs) or the functioning of multiple lncRNAs simultaneously is closely related to the biological behavior and activities of human stem cells. The lncRNA MCM3AP-AS1 was markedly upregulated during the induction of osteogenic differentiation of PDLSCs, and its level was positively correlated with ALP and Runx2 8) . In addition, the lncRNA ZFAS1 was found to suppress osteogenic differentiation and encourage adipogenic differentiation 9) .…”

Section: Introductionmentioning

confidence: 96%

lncRNA ZNF710-AS1 Acts as a ceRNA for miR-146a-5p and miR-146b-5p to Accelerate Osteogenic Differentiation of PDLSCs by Upregulating the BMP6/Smad1/5/9 Pathway

Liu¹,

2022

J. hard tissue biol.

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Multiple experimental pieces of evidence have confirmed that fully understanding the regulatory mechanisms of osteogenic differentiation of periodontal ligament stem cells (PDLSCs) can better promote and improve the ability of periodontal tissues to regenerate and alleviate periodontal diseases. This study aimed to reveal whether the long noncoding RNA (lncRNA) ZNF710-AS1 plays a role in the osteogenic differentiation of PDLSCs and its molecular mechanisms. Microarray datasets GSE159507 and GSE159508 were retrieved from the Gene Expression Omnibus database and differentially expressed genes were identified using R language (limma package). The results revealed that the expression of ZNF710-AS1 and bone morphogenetic protein 6 (BMP6) was upregulated whereas that of miR-146a-5p/miR-146b-5p was downregulated during the osteogenic differentiation of PDLSCs. PDLSCs were successfully isolated and cultured in vitro. Osteogenic and adipogenic differentiation abilities were evaluated by performing alizarin red staining and oil red O staining, respectively. Overexpression of ZNF710-AS1 significantly increased the osteogenic differentiation ability of PDLSCs by upregulating the expression of BMP6 and phosphorylation-SMAD family member 1/5/9 (p-Smad1/5/9) and competitively sponging miR-146a-5p/miR-146b-5p and acting as a competing endogenous RNA (ceRNA). This study demonstrated that ZNF710-AS1 promotes the osteogenic differentiation of PDLSCs by upregulating BMP6/Smad1/5/9 expression and acting as a ceR-NA for miR-146a-5p and miR-146b-5p.

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Overexpression of long noncoding RNA MCM3AP-AS1 promotes osteogenic differentiation of dental pulp stem cells via miR-143-3p/IGFBP5 axis

Cited by 10 publications

References 35 publications

Testosterone promotes human foreskin fibroblast growth through miR-143-3p targeting IGFBP-3

Testosterone promotes human foreskin fibroblast growth through miR-143-3p targeting IGFBP-3

Dental Pulp Stem Cells for Bone Tissue Engineering: A Literature Review

lncRNA ZNF710-AS1 Acts as a ceRNA for miR-146a-5p and miR-146b-5p to Accelerate Osteogenic Differentiation of PDLSCs by Upregulating the BMP6/Smad1/5/9 Pathway

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